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pNIC-CTHF

Plasmid

#26105

Purpose

SGC Empty backbone for bacterial expression

Depositor

Opher Gileadi

Article

Savitsky et al J Struct Biol. 2010 Oct;172(1):3-13. doi

Type

Empty backbone

Tags

TEV - His6 - Flag

Expression

Bacterial

Available Since

Oct. 18, 2010

Availability

Academic Institutions and Nonprofits only

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pJJS002

Plasmid

#188325

Purpose

PCR template for repair templates to tag a gene with a GFP and mIAA7 AID degron using homology-directed repair for C. elegans.

Depositor

Mike Boxem

Article

Sepers et al G3 (Bethesda). 2022 Aug 27. pii: 6677660

Insert

Linker::GFP::mIAA7

Expression

Bacterial

Available Since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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FRP2374

Plasmid

#127579

Purpose

Repressor plasmid for WTC 846. Encodes TetR and TetR-Tup1, Met15 selection marker

Depositor

Joerg Stelling

Article

Azizoglu et al Elife. 2021 Aug 3;10. pii: 69549. doi: 1

Insert

PRnr2_TetR-NLS-TUP1_tCyc1_PtetO7.1_TetR-NLS_tCyc1

Use

Synthetic Biology; Shuttle vector

Expression

Yeast

Available Since

Aug. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pTF101.1gw1

Plasmid

#134768

Purpose

A binary plasmid for gateway 1-Entry in pTF101.1 background (attR1 & attR2) containing double CaMV 35S promoter and TEV enhancer to drive bialaphos resistance gene (bar) for plant transformation

Depositor

Kan Wang

Article

et al Euphytica (2004) 136: 167.

Type

Empty backbone

Expression

Plant

Available Since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pMKV058

Plasmid

#133313

Purpose

BeYDV viral replicon on T-DNA backbone expressing Firefly Luc+ and WUS2

Depositor

Daniel Voytas

Article

Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Insert

Luc+. WUS2

Expression

Plant

Promoter

AtUbi10, Nos

Available Since

Dec. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pTn7-SCOUT11.Y

Plasmid

#201004

Purpose

pLv1-pUC18R6KT-miniTn7-Gm-sYFP2, GmR, ApR

Depositor

Philip Poole

Article

Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

Insert

sYFP2

Expression

Bacterial

Promoter

J23100

Available Since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

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pJJS001

Plasmid

#188324

Purpose

PCR template for repair templates to tag a gene with a GFP and mIAA7 AID degron using homology-directed repair for C. elegans.

Depositor

Mike Boxem

Article

Sepers et al G3 (Bethesda). 2022 Aug 27. pii: 6677660

Insert

mIAA7::GFP::Linker

Expression

Bacterial

Available Since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pAXY0013

Plasmid

#205582

Purpose

NpuDnaE(C)_HYG(53-341)_RUBY

Depositor

Xiaohan Yang

Article

Yuan et al Commun Biol. 2023 May 26;6(1):567. doi:

Insert

NpuDnaE(C)

Expression

Plant

Promoter

35S

Available Since

Oct. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-4T1 mouse PARG (439-959)

Plasmid

#132615

Purpose

expresses mouse PARG(439-959) in E. coli.

Depositor

Wenqing Xu

Article

Wang et al PLoS One. 2014 Jan 21;9(1):e86010. doi:

Insert

PARG (439-959)

Expression

Bacterial

Available Since

Oct. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFPC1-Spop

Plasmid

#128872

Purpose

For mammalian expression of mouse Spop

Depositor

Aimin Liu

Article

Cai et al Proc Natl Acad Sci U S A. 2016 Dec 20;11

Insert

Spop (Spop Mouse)

Tags

EGFP

Expression

Mammalian

Available Since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

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pZA69

Plasmid

#174782

Purpose

RNAi

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

Intron-containing hairpin RNA (ihpRNA)-NbZAR1

Use

RNAi

Mutation

WT

Available Since

Nov. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pPlantPOP

Plasmid

#241283

Purpose

Cloning gene libraries from oligonucleotide pools

Depositor

Sean Cutler

Article

Dvir et al Proc Natl Acad Sci U S A. 2025 Aug 5;122

Type

Empty backbone

Use

Other

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pAC-Kan-alphaGal4

Plasmid

#13421

Depositor

Keith Joung

Article

Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

Insert

RNAP alpha-Gal4

Expression

Bacterial

Available Since

Feb. 8, 2007

Availability

Academic Institutions and Nonprofits only

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pRRL.SP146.gp91.eGFP

Plasmid

#30474

Depositor

Didier Trono

Article

Barde et al Gene Ther. 2011 Nov;18(11):1087-97. doi:

Insert

SP146/gp91/eGFP

Use

Lentiviral

Available Since

Aug. 24, 2011

Availability

Academic Institutions and Nonprofits only

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FRP2365

Plasmid

#127575

Purpose

Repressor plasmid for WTC 846. Encodes TetR and TetR-Tup1, Ura3 selection marker

Depositor

Joerg Stelling

Article

Azizoglu et al Elife. 2021 Aug 3;10. pii: 69549. doi: 1

Insert

PRnr2_TetR-NLS-TUP1_tCyc1_PtetO7.1_TetR-NLS_tCyc1

Use

Synthetic Biology; Shuttle vector

Expression

Yeast

Available Since

Aug. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pMT406

Plasmid

#139384

Purpose

RPU standard, pNBU1 backbone with BsaI sites, AmpR, ErmR

Depositor

Christopher Voigt

Article

Taketani et al Nat Biotechnol. 2020 Aug;38(8):962-969.

Insert

RPU standard

Use

Synthetic Biology

Promoter

No promoter

Available Since

July 26, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5 HA-RagC

Plasmid

#99718

Purpose

Mammalian expression of HA-RagC

Depositor

David Sabatini, Kuang Shen

Article

Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

Insert

RagC (RRAGC )

Tags

HA

Promoter

CMV

Available Since

Nov. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pAG306-pTet07-nLuc

Plasmid

#199755

Purpose

Control construct of elongation reporter to quantify elongation of YFP

Depositor

Brian Zid

Article

Hou et al RNA. 2023 Dec;29(12):1928-1938. doi: 10.

Insert

Nanoluc

Expression

Yeast

Promoter

TetO7

Available Since

May 5, 2023

Availability

Academic Institutions and Nonprofits only

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pLV.SP146.gp91.eGFP.cHS4

Plasmid

#30476

Depositor

Didier Trono

Article

Barde et al Gene Ther. 2011 Nov;18(11):1087-97. doi:

Insert

SP146/gp91/eGFP/cHS4

Use

Lentiviral

Available Since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only

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pUdO2c

Plasmid

#210231

Purpose

medium-copy plasmid for bacterial expression

Depositor

François-Xavier Campbell-Valois

Article

Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

Type

Empty backbone

Expression

Bacterial

Available Since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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