We narrowed to 11,134 results for: CHL

1. pJB057

   Plasmid

   #98911

   Purpose

   Express Dronpa tagged ZapA in E coli

   Depositor

   Jie Xiao

   Article

   Buss et al PLoS Genet. 2015 Apr 7;11(4):e1005128. d

   Insert

   ZapA
   

   Tags

   Dronpa

   Expression

   Bacterial

   Promoter

   T5-lac

   Available Since

   Aug. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGP4G-TPLRD1-ccdB

   Plasmid

   #83877

   Purpose

   Destination vector for integration at yeast Trp locus with N-terminal TPL repression domain (RD) for auxin response assays

   Depositor

   Jennifer Nemhauser

   Article

   Pierre-Jerome et al Proc Natl Acad Sci U S A. 2014 Jul 1;111

   Type

   Empty backbone

   Use

   Gateway destination vector

   Available Since

   Sept. 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKS012

   Plasmid

   #65465

   Purpose

   pSC101 based plasmid where pl-tetO expression drives synthesis of construct expressing (N to C terminal) Ntag and mCherry

   Depositor

   Keith Tyo

   Article

   Sekar et al PLoS One. 2016 Feb 22;11(2):e0149746. do

   Insert

   Ntag-mCherry
   

   Use

   Synthetic Biology

   Tags

   FLAG

   Expression

   Bacterial

   Promoter

   pl-TetO

   Available Since

   June 18, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pCG-D-SpyTag-His6-E

   Plasmid

   #87375

   Purpose

   An ORF part-containing vector harbouring the SpyTag sequence with a His6 tag at its C-terminus and 4 bp overhangs specific for the ‘downstream SpyPart’ position within an ORF assembly

   Depositor

   Tom Ellis

   Article

   Gilbert et al ACS Synth Biol. 2017 Mar 7. doi: 10.1021

   Insert

   SpyTag
   

   Use

   Synthetic Biology

   Tags

   His6 tag

   Available Since

   July 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. MTK3b_004

   Plasmid

   #123785

   Purpose

   Encodes mAzamiGreen as a Type 3b part to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   mAzamiGreen
   

   Expression

   Mammalian

   Available Since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRATIO2112

   Plasmid

   #141296

   Purpose

   Gateway cloning compatible dual fluorescent ratiometric binary vector

   Depositor

   David Nelson

   Article

   Khosla et al Plant Direct. 2020 Jun 21;4(6):e00231. d

   Type

   Empty backbone

   Tags

   mScarlet-I and mVenus

   Expression

   Plant

   Promoter

   UBQ10 promoter

   Available Since

   Sept. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pT7_circRAJ31_C873U

   Plasmid

   #69932

   Purpose

   Expresses RAJ31 riboregulator encased in a permuted intron exon ribozyme, which circularises the riboregulator. This mutant is deficient in circularisation. Under control of a T7 promoter.

   Depositor

   Alfonso Jaramillo

   Article

   Rostain et al Biodes Res. 2020 Sep 12;2020:1916789. do

   Insert

   pT7_circRAJ31_C873U
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCellFree_G06

   Plasmid

   #67140

   Purpose

   Gateway destination vector for cell-free expression with C-term mCherry 8xHis tag

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al J Biotechnol. 2015 Feb 10;195:1-7. doi:

   Type

   Empty backbone

   Use

   Cell-free expression

   Tags

   HIS and mCherry

   Available Since

   Aug. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLY185

   Plasmid

   #184146

   Purpose

   RFP generator controlled by Plux2 promoter

   Depositor

   Baojun Wang

   Article

   Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:

   Insert

   rfp
   

   Use

   Synthetic Biology

   Promoter

   Plux2

   Available Since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFLIP43

    Plasmid

    #65618

    Purpose

    FRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.

    Depositor

    Wolf Frommer

    Article

    Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755

    Type

    Empty backbone

    Tags

    6His-edAFPt9-attR1 and attR2-edCer-cMyc

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    June 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJMC-2-Reverse

    Plasmid

    #197801

    Purpose

    Level 1 Module 2 destination vector (reverse orientation MoClo insert)

    Depositor

    Daniel Voytas

    Article

    Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

    Type

    Empty backbone

    Use

    Unspecified

    Available Since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. Destination Vector

    Plasmid

    #125754

    Purpose

    Destination vector for assembling gRNA fragments in the second round of assembly.

    Depositor

    Rodrigo Ledesma-Amaro

    Article

    McCarty et al ACS Synth Biol. 2019 Apr 2. doi: 10.1021

    Insert

    pTDH3-GFPdropout-tTDH1
    

    Use

    Synthetic Biology

    Expression

    Yeast

    Available Since

    July 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. Guide-Generating Vector

    Plasmid

    #125752

    Purpose

    Guide-Generating Vector for creating the Cys4-guide-scaffold structure in the first round of assembly

    Depositor

    Rodrigo Ledesma-Amaro

    Article

    McCarty et al ACS Synth Biol. 2019 Apr 2. doi: 10.1021

    Insert

    Cys4-GFPdropout-SpCas9scaffold
    

    Use

    Synthetic Biology

    Available Since

    July 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. MTK2_013

    Plasmid

    #123705

    Purpose

    Encodes a spacer (for multicistronic) as a Type 2 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    pSpacer multicistronic
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCellFree_G09

    Plasmid

    #67143

    Purpose

    Gateway destination vector for cell-free expression with N-term 8xHis sfGFP tag

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al J Biotechnol. 2015 Feb 10;195:1-7. doi:

    Type

    Empty backbone

    Use

    Cell-free expression

    Tags

    His and sfGFP

    Available Since

    Aug. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCZGY553

    Plasmid

    #110883

    Purpose

    Pmec-4 Gateway Destination vector for cloning for expression in C. elegans mechanosensory neurons

    Depositor

    Andrew Chisholm

    Article

    Ghosh-Roy et al J Neurosci. 2010 Mar 3;30(9):3175-83. do

    Insert

    Pmec-4
    

    Use

    Gateway destination vector

    Available Since

    Oct. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBzCas13b

    Plasmid

    #164862

    Purpose

    Inducible expression of BzCas13b nuclease in bacteria.

    Depositor

    Chase Beisel

    Article

    Wandera et al Methods Mol Biol. 2022;2404:135-153. doi

    Insert

    Bergeyella zoohelcum Cas13b
    

    Expression

    Bacterial

    Promoter

    T7 Promoter

    Available Since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLPS-hAkt1-gfp

    Plasmid

    #49388

    Purpose

    mammalian expression of human Akt1 fused to EGFP

    Depositor

    Randall McKinnon

    Insert

    Akt1 (AKT1 Human)
    

    Use

    Cre/Lox; Acceptor vector

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    tga to cca at nt 1725 with respect to GenBank ID …

    Promoter

    CMV

    Available Since

    Nov. 21, 2013

    Availability

    Academic Institutions and Nonprofits only

19. pReporter_10

    Plasmid

    #60570

    Purpose

    A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).

    Depositor

    Christopher Voigt

    Article

    Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

    Insert

    Reporter_10
    

    Expression

    Bacterial

    Promoter

    BBa_J23119

    Available Since

    March 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBbS15c-RFP

    Plasmid

    #126198

    Purpose

    Constitutive expression vector for Escherichia coli. BglBrick compatible.

    Depositor

    Nigel Scrutton

    Article

    Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

    Insert

    RFP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    J23100

    Available Since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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