We narrowed to 8,708 results for: sgRNA

1. pLentiCRISPRv2 Neo sgTREX1

   Plasmid

   #127645

   Purpose

   Knock-out of human TREX1 with NeoR

   Depositor

   Nicolas Manel

   Article

   Gratia et al J Exp Med. 2019 May 6;216(5):1199-1213.

   Insert

   TREX1 sgRNA (TREX1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. qTAG-C-moxGFP-Puro-LAMP1

   Plasmid

   #207788

   Purpose

   Donor template for moxGFP-2A-Puro insertion into the C-terminus of the LAMP1 locus. For lysosome visualization.To be co-transfected with sgRNA plasmid px330-LAMP1 (Addgene #207787)

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   LAMP1 Homology Arms flanking a moxGFP-Puro Cassette (LAMP1 Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 1, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pLenti-Puro-sgNUAK1

   Plasmid

   #138692

   Purpose

   Expresses a human NUAK1-targeting sgRNA and Cas9

   Depositor

   Reuben Shaw

   Article

   Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

   Insert

   sgNUAK1 human (NUAK1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   hU6

   Available since

   Feb. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. Lenti_SaCRISPR_GFP

   Plasmid

   #118636

   Purpose

   To clone sgRNA for SaCas9 (SauCas9)

   Depositor

   Christopher Vakoc

   Article

   Lu et al Cancer Cell. 2018 Dec 10;34(6):970-981.e

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   Jan. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCR1163

   Plasmid

   #111099

   Purpose

   Expresses FANCD2_+_10068200.23-P1P2 sgRNA in pCR1068

   Depositor

   Jacob Corn

   Article

   Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

   Insert

   FANCD2 gRNA (FANCD2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. Intron-Tagging-mScarlet-Frame1-Parental-Minicircle

   Plasmid

   #216125

   Purpose

   Parental minicircle containing mScarlet-I flanked by a splice acceptor and splice donor. Used with other intron tagging plasmids for placing the mScarlet tag as a synthetic exon into frame 1 introns of target genes.

   Depositor

   Stefan Kubicek

   Article

   Reicher et al Nat Cell Biol. 2024 May;26(5):745-756. d

   Insert

   sgRNA-SA-(GGGGS)x4-mScarlet-I-(GGGGS)x4-SD
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. qTAG-C-mStayGold-Puro-CANX

   Plasmid

   #227281

   Purpose

   Donor template for mStayGold-2A-Puro insertion into the C-terminus of the CANX locus. For cilia visualization. To be co-transfected with sgRNA plasmid px330-CANX (Addgene #227279)

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   CANX Homology Arms flanking a mStayGold-2A-Puro Cassette (CANX Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Promoterless

   Available since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti-Puro-sgNUAK2

   Plasmid

   #138693

   Purpose

   Expresses a human NUAK2-targeting sgRNA and Cas9

   Depositor

   Reuben Shaw

   Article

   Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

   Insert

   sgNUAK2 human (NUAK2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   hU6

   Available since

   Feb. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEJS2602_AAV-Nme2-ABE8e-i1(V106W)-U6-2xSapI

   Plasmid

   #201684

   Purpose

   All-in-one AAV plasmid expressing Nme2-ABE8e-i1(V106W) and U6 sgRNA cassette. SapI enables cloning new spacers.

   Depositor

   Erik Sontheimer

   Article

   Bamidele et al Nat Commun. 2024 Feb 17;15(1):1458. doi:

   Insert

   Nme2-ABE8e-i1 (V106W)
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U1a

   Available since

   Jan. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV.Ple155-CI-mRuby2-W3SL_BbsI(GGA)

    Plasmid

    #231364

    Purpose

    Ple155-driven mRuby2, containing cassette for BbsI-based Golden Gate assembly (e.g. of sgRNA cassette(s)). Can be used as 'no guide' control.

    Depositor

    Viviana Gradinaru

    Article

    Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

    Insert

    mRuby2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. qTAG-N-Solo-mStayGold-CETN2

    Plasmid

    #227292

    Purpose

    Donor template for mStayGold insertion into the N-terminus of the CETN2 locus. For centriole visualization. To be co-transfected with sgRNA plasmid px330-CETN2 (Addgene #227291)

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    CETN2 Homology Arms flanking a mStayGold Tag (CETN2 Human)
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Promoterless

    Available since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. qTAG-N-Solo-mStayGold-CENPA

    Plasmid

    #227283

    Purpose

    Donor template for mStayGold insertion into the N-terminus of the CENPA locus. For centromere visualization. To be co-transfected with sgRNA plasmid px330-CENPA (Addgene #227282)

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    CENPA Homology Arms flanking a mStayGold Tag (CENPA Human)
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Promoterless

    Available since

    Nov. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJMP1183

    Plasmid

    #119254

    Purpose

    rfp "test" strain

    Depositor

    Oren Rosenberg, Carol Gross, Jason Peters

    Article

    Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

    Insert

    sgRNA NT1/RR1 (rfp)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D10A and H840A

    Promoter

    Available since

    Jan. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. qTAG-C-Solo-mStayGold-CANX

    Plasmid

    #227280

    Purpose

    Donor template for mStayGold insertion into the C-terminus of the CANX locus. For ER visualization. To be co-transfected with sgRNA plasmid px330-CANX (Addgene #227279)

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    CANX Homology Arms flanking a mStayGold Tag (CANX Human)
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Promoterless

    Available since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. qTAG-N-Puro-moxGFP-LMNB1

    Plasmid

    #207773

    Purpose

    Donor template for Puro-2A-moxGFP insertion into the N-terminus of the LMNB1 locus for nuclear envelope visualization. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 Addgene #207770

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    LMNB1 Homology Arms flanking a Puro-moxGFP Cassette (LMNB1 Human)
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. Intron-Tagging-EGFP-Frame0-Parental-Minicircle

    Plasmid

    #216124

    Purpose

    Parental minicircle containing EGFP flanked by a splice acceptor and splice donor. Used with other intron tagging plasmids for placing the EGFP tag as a synthetic exon into frame 0 introns of target genes.

    Depositor

    Stefan Kubicek

    Article

    Reicher et al Nat Cell Biol. 2024 May;26(5):745-756. d

    Insert

    sgRNA-SA-(GGGGS)x4-EGFP-(GGGGS)x4-SD
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. qTAG-N-Puro-mNeon-ACTB

    Plasmid

    #207752

    Purpose

    Donor template for Puro-2A-mNeon insertion into the N-terminus of the ACTB locus for actin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-ACTB Addgene #207748

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    ACTB Homology Arms flanking a Puro-mNeon Cassette (ACTB Human)
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJMP1237

    Plasmid

    #119262

    Purpose

    for cloning new sgRNAs

    Depositor

    Oren Rosenberg, Carol Gross, Jason Peters

    Article

    Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

    Insert

    dcas9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D10A and H840A

    Promoter

    Available since

    Jan. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pInducer20 CARD8-HA PAM E274R

    Plasmid

    #169984

    Purpose

    Tet/Dox-inducible mammalian expression of CARD8 that cannot bind DPP9 due to a mutation at site I (E274R) with sgRNA binding site mutations

    Depositor

    Daniel Bachovchin

    Article

    Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

    Insert

    CARD8 (CARD8 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    PAM, E274R

    Promoter

    Available since

    May 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pNICKclos1.0

    Plasmid

    #73639

    Purpose

    Genome editing for gene pyrE (CAC-002) in Clostridium acetobutylicum ATCC 824

    Depositor

    Sheng Yang

    Article

    Li et al Biotechnol J. 2016 May 23. doi: 10.1002/

    Inserts

    Cas9 nickase
    sGRNA to pyrE
    

    Use

    E.coli-clostridium shuttle vector

    Tags

    Expression

    Mutation

    D10A

    Promoter

    j23119 and ptb

    Available since

    June 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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