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pBpuro_KLF4_ER

Plasmid

#34609

Depositor

Michael Ruppert

Publication

Foster et al Oncogene. 2005 Feb 24;24(9):1491-500.

Insert

KLF4 (KLF4 Human)

Use

Retroviral

Tags

Myc epitope tag and estrogen receptor fusion

Expression

Mammalian

Promoter

LTR

Available since

Jan. 24, 2012

Availability

Academic Institutions and Nonprofits only
pJKR-H-mphR

Plasmid

#62559

Purpose

Macrolide biosensor. High copy plasmid that expresses GFP proportional to macrolide concentration.

Depositor

George Church

Publication

Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

Insert

GFP

Expression

Bacterial

Available since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCB-2

Plasmid

#210388

Purpose

All-in-one cumate-based inducible CRISPRi plasmid for use in Streptomyces

Depositor

Gilles van Wezel

Publication

Bai et al ACS Synth Biol. 2023 Oct 6. doi: 10.1021

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Inserts

dCas9
CymR
sgRNA cassette

Use

CRISPR; Integrative vector for use in streptomyces

Mutation

BsaI-flanked RFP cassette inserted in place of sp…

Promoter

SP11 and SP30

Available since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pET-His6-FnCas9GFP

Plasmid

#130966

Purpose

expression of FnCas9-GFP in bacterial cells

Depositor

Debojyoti Chakraborty

Publication

Acharya et al Proc Natl Acad Sci U S A. 2019 Oct 15;11

Insert

HA-NLS-FnCas9

Use

CRISPR

Tags

6xHis-mEGFP-TEV-HA-NLS

Expression

Bacterial

Promoter

T7

Available since

Nov. 7, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCMV-myc3-Mdm2 p60

Plasmid

#52059

Purpose

expression of truncated Mdm2 in mammalian cells

Depositor

Tyler Jacks, Trudy Oliver

Publication

Oliver et al Mol Cell. 2011 Jul 8;43(1):57-71. doi: 1

Insert

Mdm2 p60 (truncated) (MDM2 Human)

Tags

myc3

Expression

Mammalian

Mutation

p60 (truncated at D367, DVDP motif)

Promoter

CMV

Available since

March 20, 2014

Availability

Academic Institutions and Nonprofits only
pCMV-myc3-Mdm2 D367A

Plasmid

#52058

Purpose

expression of non-cleavable Mdm2 in mammalian cells

Depositor

Tyler Jacks, Trudy Oliver

Publication

Oliver et al Mol Cell. 2011 Jul 8;43(1):57-71. doi: 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Mdm2 D367A (MDM2 Human)

Tags

myc3

Expression

Mammalian

Mutation

D367A

Promoter

CMV

Available since

March 13, 2014

Availability

Academic Institutions and Nonprofits only
-962 human cyclin D1 promoter AP-1 site mutant pGL3Basic

Plasmid

#32728

Depositor

Frank McCormick

Publication

Tetsu et al Nature. 1999 Apr 1;398(6726):422-6.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

CCND1 promoter (CCND1 Human)

Use

Luciferase; Luciferase reporter gene

Mutation

-947 AP-1 binding site TGAGTCA deletion

Promoter

-962 CCND1 promoter

Available since

Jan. 27, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pclbw-eGFPomp25

Plasmid

#58424

Purpose

Mammalian expression of GFP targeted to the mitochondrial outer membrane

Depositor

David Chan

Publication

Mishra et al Cell Metab. 2014 Apr 1;19(4):630-41. doi

Insert

eGFP-omp25 (Synj2bp Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

contains aa's 109-145 of mouse omp25

Promoter

CMV/b-actin

Available since

Dec. 15, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
GFP-CYLD-STOP

Plasmid

#60077

Purpose

N-terminal GFP tagged CYLD with stop codon, driven by CMV promoter

Depositor

Stephen Elledge

Publication

Stegmeier et al Proc Natl Acad Sci U S A. 2007 May 22. 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Cylindromatosis (CYLD Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available since

Aug. 26, 2015

Availability

Academic Institutions and Nonprofits only

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p2.1 (pAGL2A)

Plasmid

#27563

Depositor

Gregg Semenza

Publication

Semenza et al J Biol Chem. 1996 Dec 20. 271(51):32529-

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ENO1 promoter (ENO1 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available since

Oct. 19, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pFRT_TO_FlagHA_ELAVL1

Plasmid

#106104

Purpose

Inducible gene expression of FlagHA ELAVL1

Depositor

Thomas Tuschl

Publication

Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

Insert

ELAVL1 (ELAVL1 Human)

Tags

Flag and HA

Expression

Mammalian

Available since

Feb. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pMSCV mouse Hic-5

Plasmid

#11190

Depositor

Bruce Spiegelman

Publication

Drori et al Genes Dev. 2005 Feb 1. 19(3):362-75.

Insert

Hic-5 (Tgfb1i1 Mouse)

Use

Retroviral

Expression

Mammalian

Available since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only
LGshp53CreER (self deleting)

Plasmid

#33341

Depositor

Scott Lowe

Publication

Zhao et al Genes Dev. 2010 Jul 1;24(13):1389-402.

Insert

CreERT2

Available since

Feb. 1, 2012

Availability

Academic Institutions and Nonprofits only

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pSH624-ssr

Plasmid

#199241

Purpose

Expresses the gene codying for the Ssr recombinase from Pseudomonas putida DOT-T1E

Depositor

Susanne Häußler

Publication

Pankratz et al Nat Protoc. 2023 Oct 5. doi: 10.1038/s41

Insert

single-stranded DNA recombineering

Expression

Bacterial

Promoter

lacIq-Ptrc

Available since

Oct. 10, 2023

Availability

Academic Institutions and Nonprofits only

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PCDH-WT-ALKBH5-3xFLAG

Plasmid

#203736

Purpose

Overexpression of ALKBH5-WT

Depositor

Jianjun Chen

Publication

Shen et al Cell Stem Cell. 2020 Jul 2;27(1):64-80.e

Insert

ALKBH5 (ALKBH5 Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Available since

July 28, 2023

Availability

Academic Institutions and Nonprofits only

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hTRIM65_EGFP_N1

Plasmid

#54320

Purpose

Mammalian expression vector for human TRIM65 fusion protein with c-terminal EGFP.

Depositor

Martin Dorf

Publication

Li et al Proc Natl Acad Sci U S A. 2014 May 13;11

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

TRIM65 (TRIM65 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available since

July 1, 2014

Availability

Academic Institutions and Nonprofits only

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pCMV3FLAG_hGTPBP4

Plasmid

#53974

Purpose

Mammalian expression plasmid for human GTPBP4 with n-terminal 3xFLAG epitope fused.

Depositor

Martin Dorf

Publication

Li et al Proc Natl Acad Sci U S A. 2014 May 13;11

Insert

GTPBP4 (GTPBP4 Human)

Tags

3x FLAG

Expression

Mammalian

Promoter

CMV

Available since

July 1, 2014

Availability

Academic Institutions and Nonprofits only

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pET28a-His-hPKM2

Plasmid

#42515

Depositor

Dimitrios Anastasiou

Publication

Anastasiou et al Science. 2011 Dec 2;334(6060):1278-83. d

Insert

Pyruvate Kinase M2 (PKM Human)

Tags

His

Expression

Bacterial

Available since

March 7, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pmiRA1-MYB

Plasmid

#141120

Purpose

Lentiviral overexpression of MYB in mammalian cells.

Depositor

Jianjun Chen

Publication

Weng et al Cell Stem Cell. 2018 Feb 1;22(2):191-205

Insert

MYB (MYB Human)

Use

Lentiviral

Expression

Mammalian

Available since

Dec. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+_FH-AGO2-WT

Plasmid

#92006

Purpose

Expresses FLAG-HA-AGO2 (WT)

Depositor

Joshua Mendell

Publication

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

AGO2 (AGO2 Human)

Tags

FLAG-HA

Expression

Mammalian

Available since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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