We narrowed to 22,844 results for: Sis

1. His-DNPH1

   Plasmid

   #203224

   Purpose

   For protein expression. Human DNPH1 is an N-glycosidase with specificity toward 5-hydroxymethyl uridine monophosphate. Pet28a plasmid, KAN resistant, N-terminal TEV-cleavable 6xHis tag.

   Depositor

   Vern Schramm

   Article

   Wagner et al Anal Biochem. 2023 Jul 1;672:115171. doi

   Insert

   DNPH1 (DNPH1 Human)
   

   Use

   Tags

   His tag (with TEV cleavage site)

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   July 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-FLEXfrt-SaCas9-U6-sgGabrg1

   Plasmid

   #124870

   Purpose

   Mutagenesis of Gabrg1

   Depositor

   Larry Zweifel

   Article

   Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

   Insert

   Gabrg1 (Gabrg1 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEF1α-M2rtTA-T2A-Puro

   Plasmid

   #169175

   Purpose

   piggyBac co-transfection transposon containing M2rtTA promoter linked with Puromycin resistance gene, through T2A with GSG linker, subcloned between ends of two ITRs. Pairs with #169174

   Depositor

   Igor Slukvin

   Article

   Jung et al Cell Rep. 2021 Feb 16;34(7):108758. doi:

   Insert

   M2rtTA-T2A-Puro
   

   Use

   Piggybac transposon

   Tags

   Expression

   Mammalian

   Mutation

   See Depositor Comments Below

   Promoter

   EF1a

   Available Since

   May 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pENTR-AtTAS1c-D2-B/c

   Plasmid

   #137883

   Purpose

   Gateway-compatible entry vector for direct cloning of synthetic trans-acting siRNAs into Arabidopsis thaliana TAS1c precursor at position downstream 3'D1[+]

   Depositor

   Alberto Carbonell

   Article

   Lopez-Dolz et al Nucleic Acids Res. 2020 May 12. pii: 583

   Insert

   AtTAS1c-D2-B/c
   

   Use

   Gateway-compatible entry clone

   Tags

   Expression

   Mutation

   A. thaliana TAS1c precursor sequence including a …

   Promoter

   Available Since

   May 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. p221- purVSG117UTR

   Plasmid

   #59732

   Purpose

   Inserts a puromycin resistance gene and a VSG117 gene into the VSG221 expression site of T.brucei downstream of the expression site promoter.

   Depositor

   Gloria Rudenko

   Article

   Smith et al PLoS One. 2009 Oct 26;4(10):e7532. doi:

   Insert

   VSG117 flanked upstream and downstream by sequences homologous to the VSG221 expression site
   

   Use

   For homologous recombination into vsg221 expressi…

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-FLEX-SaCas9-U6-sgGabrg1

   Plasmid

   #124856

   Purpose

   Mutagenesis of Gabrg1

   Depositor

   Larry Zweifel

   Article

   Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

   Insert

   Gabrg1 (Gabrg1 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMADM-alpha

   Plasmid

   #36890

   Depositor

   Liqun Luo

   Article

   Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

   Inserts

   GFP
   tdTomato-3Myc
   beta-globin intron
   Neo
   ATG (T)
   GFP
   

   Use

   Tags

   3 Myc tags

   Expression

   Mammalian

   Mutation

   deleted nucleotides after nucleotide 274, deleted…

   Promoter

   CAG (chicken beta actin promoter and CMV enhancer…

   Available Since

   Aug. 17, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-FLEXfrt-SaCas9-U6-sgGrin2b

   Plasmid

   #124864

   Purpose

   Mutagenesis of Grin2b

   Depositor

   Larry Zweifel

   Article

   Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

   Insert

   Grin2b (Grin2b Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPKm-245

   Plasmid

   #90503

   Purpose

   Expresses HO1, PCYA, FD and FNR, required for PCB synthesis. pSIN - EF-1alpha - MTS - tHO1 - P2A - MTS - tPCYA - P2A - MTS - tFD - P2A - MTS - tFNR

   Depositor

   Todd Coleman

   Article

   Kyriakakis et al ACS Synth Biol. 2018 Feb 16;7(2):706-717

   Insert

   mito-tHO1, mito-tPCYA, mito-tFD, mito-tFNR
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   April 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pPKm-244

    Plasmid

    #90502

    Purpose

    Expresses HO1, PCYA, FD and FNR, required for PCB synthesis. pSIN - EF-1alpha - MTS - tHO1 - P2A - MTS - tPCYA - IRES - MTS - tFD - P2A - MTS - tFNR

    Depositor

    Todd Coleman

    Article

    Kyriakakis et al ACS Synth Biol. 2018 Feb 16;7(2):706-717

    Insert

    mito-tHO1, mito-tPCYA, mito-tFD, mito-tFNR
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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