We narrowed to 31,171 results for: LIS

1. HA-Rab8a-DN (T22N)

   Plasmid

   #101049

   Purpose

   Expresses HA-tagged human Rab8-DN (T22N) in mammalian cells

   Depositor

   Ken-Ichi Takemaru

   Article

   Burke et al J Cell Biol. 2014 Oct 13;207(1):123-37.

   Insert

   Rab8a (RAB8A Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   T22N

   Promoter

   CMV

   Available since

   Oct. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLVX-TetOne-Puro-PFDN2

   Plasmid

   #202664

   Purpose

   Tet-ON 3rd generation expression vector with inducible expression of PFDN2 and a puromycin selection cassette

   Depositor

   Jason Sheltzer

   Article

   Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

   Insert

   prefoldin subunit 2 (PFDN2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. LentiCRISPR v2 RB1 sgRNA #2

   Plasmid

   #202519

   Purpose

   Expressing Cas9 and sgRNA targeting human RB1 gene

   Depositor

   Ming Chen

   Article

   Wang et al J Clin Invest. 2023 Mar 16:e166647. doi:

   Insert

   RB1 sgRNA (RB1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   June 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. 33-G-X5-pEF1/V5-His

   Plasmid

   #111605

   Purpose

   Express human 33-G-X5 in 293T cells

   Depositor

   Timothy Springer

   Article

   Qin et al Cell. 2018 Jun 28;174(1):156-171.e16. do

   Insert

   human LRRC33 and GARP chimeria (NRROS Human)
   

   Use

   Tags

   Flag tag

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   July 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pYLsgRNA-OsU3m/LacZ

   Plasmid

   #66192

   Purpose

   cloning of sgRNAs for expression in plants

   Depositor

   Yao-Guang Liu

   Article

   Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

   Type

   Empty backbone

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   Promoter

   OsU3m (SpeI site destroyed)

   Available since

   Aug. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCFJ1955

   Plasmid

   #84830

   Purpose

   Minimos transposon with Peft-3:tdTomato:tbb-2 3'UTR and cbr-unc-119 selection. tdTomato was optimized for C. elegans, contains 2x NLS (SV40/egl-13), and synthetic introns

   Depositor

   Andrew Fire

   Article

   Frokjaer-Jensen et al Cell. 2016 Jul 14;166(2):343-57. doi: 10

   Insert

   C. elegans codon optimized tdTomato
   

   Use

   Tags

   Expression

   Bacterial and Worm

   Mutation

   Promoter

   Peft-3

   Available since

   Dec. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUC19_TELO2_dTAG

   Plasmid

   #224380

   Purpose

   Generates TELO2 dTAG N terminal knock-in cell lines

   Depositor

   Junjie Chen

   Article

   Huang et al Mol Cell. 2023 Aug 3;83(15):2810-2828.e6

   Insert

   TELO2 (TELO2 Human)
   

   Use

   Tags

   dTAG

   Expression

   Bacterial

   Mutation

   Synonymous mutation

   Promoter

   Available since

   Oct. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUC19_GNB1L_dTAG

   Plasmid

   #224378

   Purpose

   Generates GNB1L dTAG C terminal knock-in cell lines

   Depositor

   Junjie Chen

   Article

   Huang et al Mol Cell. 2023 Aug 3;83(15):2810-2828.e6

   Insert

   GNB1L (GNB1L Human)
   

   Use

   Tags

   dTAG

   Expression

   Bacterial

   Mutation

   Synonymous mutation

   Promoter

   Available since

   Oct. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDH-CMV-mouse Gfi1-GFP

   Plasmid

   #91892

   Purpose

   3rd generation lentiviral expression of mouse Gfi1

   Depositor

   Martine Roussel

   Article

   Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

   Insert

   Gfi1 (Gfi1 Mouse)
   

   Use

   Lentiviral

   Tags

   IRES-EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Unknown

   Available since

   June 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. scAAV-Mlc2v-MPRA

    Plasmid

    #182649

    Purpose

    Self complementary AAV vector for high-throughput in vivo measurement of enhancer activity by massively parallel reporter assay

    Depositor

    William Pu

    Article

    Akerberg et al Nat Commun. 2019 Oct 28;10(1):4907. doi:

    Insert

    mCherry
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    rat Mlc2v

    Available since

    May 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pNL-CEF-CD79A-RFP

    Plasmid

    #187003

    Purpose

    Expression of human CD79A as RFP fusion protein

    Depositor

    Hermann Eibel

    Article

    Sevdali et al Cell Rep. 2022 Jun 28;39(13):111019. doi

    Insert

    CD79A (CD79A Human)
    

    Use

    Lentiviral

    Tags

    tagRFP

    Expression

    Mutation

    Promoter

    Available since

    July 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-FLEX-hKCNQ3(R230C)-P2A-EGFP

    Plasmid

    #209194

    Purpose

    Cre-dependent expression of Kcnq3 with R230C mutation and GFP from the same transcript

    Depositor

    Larry Zweifel

    Article

    Gamal El-Din et al Proc Natl Acad Sci U S A. 2021 Nov 9;118

    Insert

    Kcnq3 (KCNQ3 Human)
    

    Use

    AAV

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    R230 to C230

    Promoter

    CMV

    Available since

    Jan. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. Lenti-BaeI-Pgk-Cre

    Plasmid

    #173663

    Purpose

    Vector with BaeI site to allow cloning of custom sgRNA into vector containing Cre-recombinase

    Depositor

    Charles Swanton

    Article

    Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

    Insert

    BaeI site
    

    Use

    CRISPR, Cre/Lox, and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. GNAS_pcDNA6.2/EmGFP-Bsd

    Plasmid

    #176976

    Purpose

    Mammalian expression vector encoding GNAS and EmGFP-Bsd

    Depositor

    Roger Reeves

    Article

    Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

    Insert

    GNAS (GNAS Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pINDUCER12 (miR-RUL)

    Plasmid

    #46935

    Purpose

    Inducible lentiviral gene silencing vector. Insert PheSGly294, (XhoI to EcoRI;1.4kb),can be replaced w mir30 based hairpin of interest

    Depositor

    Thomas Westbrook

    Article

    Meerbrey et al Proc Natl Acad Sci U S A. 2011 Mar 1;108

    Insert

    PheS Gly294
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLVX-Puro-mGSDME F2A

    Plasmid

    #154878

    Purpose

    Expresses mGSDME F2A in mammalian cells

    Depositor

    Judy Lieberman

    Article

    Zhang et al Nature. 2020 Mar;579(7799):415-420. doi:

    Insert

    GSDME (Gsdme Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    changes Phenylalanine 2 to Alanine

    Promoter

    CMV

    Available since

    July 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGLO-GFP-3UAG

    Plasmid

    #82501

    Purpose

    Expresses GFP with 3 UAG codons at the amino acid position 3, 151, and 153

    Depositor

    Chang Liu

    Article

    Schwessinger et al Integr Biol (Camb). 2016 Apr 18;8(4):542

    Insert

    Green Fluorescence Protein with 3 UAG codons
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Added an UAG codon at the amino acid position 3, …

    Promoter

    AraBAD

    Available since

    Sept. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. AAVS1_gRNA

    Plasmid

    #196139

    Purpose

    gRNA targeting the AAVS1 locus in a third generation Cas9 backbone with GFP

    Depositor

    Jason Sheltzer

    Article

    Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

    Insert

    AAVS1 gRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. cyclin D1-HA-pBabe-Hygro

    Plasmid

    #22703

    Depositor

    William Kaelin

    Article

    Zhang et al Cancer Cell. 2009 Nov 6. 16(5):413-24.

    Insert

    cyclin D1 (CCND1 Human)
    

    Use

    Retroviral

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    wt cDNA

    Promoter

    Available since

    Dec. 3, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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20. pC178: pAAV.U6.SapI.CMV.Cas13bt3

    Plasmid

    #204215

    Purpose

    Plasmid AAV vector expressing Cas13bt3 and hU6-driven expression of guide RNAs. Contains SapI sites for guide cloning flanked by 5' and 3' full-length DRs.

    Depositor

    Guei-Sheung Liu

    Article

    Kumar et al Proc Natl Acad Sci U S A. 2024 Nov 5;121

    Insert

    Cas13bt3 and U6.SapI sgRNA cloning site
    

    Use

    AAV and CRISPR

    Tags

    HA and NLS

    Expression

    Mammalian

    Mutation

    Codon optimisation by GenScript tool

    Promoter

    CMV/U6

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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