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pEF myc Gal cycT2b (P#1719)

Plasmid

#14600

Depositor

Matija Peterlin

Article

Kurosu et al Gene. 2004 Dec 8. 343(1):173-9.

Insert

cycT2 (CCNT2 Human)

Tags

Gal and myc

Expression

Mammalian

Available Since

June 30, 2008

Availability

Academic Institutions and Nonprofits only
pISH-OCAM

Plasmid

#15932

Depositor

Peter Mombaerts

Article

Li et al Nature. 2004 Mar 25. 428(6981):393-9.

Insert

OCAM (Ncam2 Mouse)

Available Since

Jan. 24, 2012

Availability

Academic Institutions and Nonprofits only
pRK5-Myc-TEAD1-Y406A

Plasmid

#33047

Depositor

Kunliang Guan

Article

Li et al Genes Dev. 2010 Feb 1;24(3):235-40.

Insert

TEAD1 (TEAD1 Human)

Tags

Myc

Expression

Mammalian

Mutation

Y406A

Available Since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only
pBABE FoxE1

Plasmid

#26350

Depositor

Matthew Meyerson

Article

Bass et al Nat Genet. 2009 Nov . 41(11):1238-42.

Insert

FOXE1 (FOXE1 Human)

Use

Retroviral

Expression

Mammalian

Available Since

Sept. 22, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223 SARS-CoV-2 NSP6_nostop

Plasmid

#149309

Purpose

Gateway-compatible Entry vector, with insert of NSP6 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.

Depositor

Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

NSP6 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway Cloning

Mutation

Many synonymous changes due to codon optimization

Available Since

May 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pTRIP-CMV

Plasmid

#235534

Purpose

To express GFP

Depositor

Nicolas Manel

Article

Lahaye et al Cell. 2025 May 29:S0092-8674(25)00556-2.

Type

Empty backbone

Use

Lentiviral

Available Since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5-Myc-TEAD1-Y406A

Plasmid

#33047

Depositor

Kunliang Guan

Article

Li et al Genes Dev. 2010 Feb 1;24(3):235-40.

Insert

TEAD1 (TEAD1 Human)

Tags

Myc

Expression

Mammalian

Mutation

Y406A

Available Since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only
pETM6-P.accABCD

Plasmid

#66866

Purpose

Encodes accA, accB, accC, accD in Pseudo-operon configuration.

Depositor

Mattheos Koffas

Article

Xu et al Nat Commun. 2013;4:1409. doi: 10.1038/nc

Inserts

accA
accB
accC
accD

Use

Synthetic Biology

Expression

Bacterial

Promoter

T7

Available Since

July 9, 2015

Availability

Academic Institutions and Nonprofits only

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pCAD39_mIRG1_4-462_pvp008

Plasmid

#124873

Purpose

Expresses mouse CAD (Irg1) in E.coli

Depositor

Konrad Buessow

Article

Chen et al Proc Natl Acad Sci U S A. 2019 Sep 23. p

Insert

cis-aconitate decarboxylase (Acod1 Mouse)

Tags

StrepTag and TEV site

Expression

Bacterial

Mutation

deleted amino acids 1-3 and 463-end.

Promoter

T7

Available Since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pRecLV105 MEF2C

Plasmid

#107240

Purpose

A lentiviral vector for expression in mammalian cells encoding MEF2C insert

Depositor

Alex Kentsis

Article

Brown et al Cancer Discov. 2018 Apr;8(4):478-497. do

Insert

MEF2C

Use

Retroviral

Expression

Mammalian

Available Since

March 28, 2018

Availability

Academic Institutions and Nonprofits only

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pINDUCER20 MEF2C S222D

Plasmid

#107234

Purpose

MEF2C phosphomimetic mutant serine to aspartic acid

Depositor

Alex Kentsis

Article

Brown et al Cancer Discov. 2018 Apr;8(4):478-497. do

Insert

MEF2C

Use

Lentiviral

Expression

Mammalian

Available Since

March 16, 2018

Availability

Academic Institutions and Nonprofits only

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pETM6-P.accABCD

Plasmid

#66866

Purpose

Encodes accA, accB, accC, accD in Pseudo-operon configuration.

Depositor

Mattheos Koffas

Article

Xu et al Nat Commun. 2013;4:1409. doi: 10.1038/nc

Inserts

accA
accB
accC
accD

Use

Synthetic Biology

Expression

Bacterial

Promoter

T7

Available Since

July 9, 2015

Availability

Academic Institutions and Nonprofits only

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pDONR223 SARS-CoV-2 NSP6_nostop

Plasmid

#149309

Purpose

Gateway-compatible Entry vector, with insert of NSP6 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.

Depositor

Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

NSP6 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway Cloning

Mutation

Many synonymous changes due to codon optimization

Available Since

May 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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TDP-REGv2(mScarlet-FLAG)#10 in pTwist-CMV backbone

Plasmid

#216154

Purpose

Expresses mScarlet in cells with TDP-43 loss-of-function. Leaky but very sensitive to mild TDP-43 loss of function. It uses a cryptic exon. [Code 'B5'] Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor Comments

Depositor

Pietro Fratta

Article

Wilkins et al Science. 2024 Oct 4;386(6717):61-69. doi

Insert

mScarlet with cryptic exon and C-terminal FLAG

Expression

Mammalian

Available Since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

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pLPC-FLAG.Mm.KAT5

Plasmid

#184647

Purpose

Retroviral expression of mouse Kat5

Depositor

Agnel Sfeir

Article

Barry et al Genes Dev. 2022 Feb 24. pii: gad.349039.

Insert

Kat5 (Kat5 Mouse)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

May 24, 2022

Availability

Academic Institutions and Nonprofits only

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TDP-REGv2(mScarlet-FLAG)#10 in pTwist-CMV backbone

Plasmid

#216154

Purpose

Expresses mScarlet in cells with TDP-43 loss-of-function. Leaky but very sensitive to mild TDP-43 loss of function. It uses a cryptic exon. [Code 'B5'] Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor Comments

Depositor

Pietro Fratta

Article

Wilkins et al Science. 2024 Oct 4;386(6717):61-69. doi

Insert

mScarlet with cryptic exon and C-terminal FLAG

Expression

Mammalian

Available Since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

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pLPC-FLAG.Mm.KAT5

Plasmid

#184647

Purpose

Retroviral expression of mouse Kat5

Depositor

Agnel Sfeir

Article

Barry et al Genes Dev. 2022 Feb 24. pii: gad.349039.

Insert

Kat5 (Kat5 Mouse)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

May 24, 2022

Availability

Academic Institutions and Nonprofits only

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P-Ef1a-RPL22-3XHA-P2A-mCherry-T2A-Neo

Plasmid

#170321

Purpose

Expresses HA and mCherry tagged RPL22, neomycin resistance

Depositor

Hemali Phatnani

Article

Gregory et al Cells. 2020 Jun 5;9(6). pii: cells906140

Insert

RPL22 (RPL22 Human)

Use

Lentiviral

Mutation

None

Available Since

May 17, 2021

Availability

Academic Institutions and Nonprofits only

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P-Ef1a-RPL22-3XHA-P2A-mCherry-T2A-Neo

Plasmid

#170321

Purpose

Expresses HA and mCherry tagged RPL22, neomycin resistance

Depositor

Hemali Phatnani

Article

Gregory et al Cells. 2020 Jun 5;9(6). pii: cells906140

Insert

RPL22 (RPL22 Human)

Use

Lentiviral

Mutation

None

Available Since

May 17, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR223_NRAS_p.Q61L

Plasmid

#81674

Purpose

Gateway Donor vector containing NRAS, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

NRAS (NRAS Human)

Use

Gateway Cloning

Mutation

Q61L

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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