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pSc1

Plasmid

#80436

Purpose

Expresses eGFP along with an U6 promoter driven gRNA which cleaves the plasmid in-cell

Depositor

Ervin Welker

Article

Talas et al DNA Res. 2017 Jun 30. doi: 10.1093/dnare

Inserts

sp Cas9 gRNA
eGFP

Use

CRISPR

Expression

Mammalian

Promoter

CMV and U6

Available Since

Aug. 11, 2016

Availability

Academic Institutions and Nonprofits only

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MSCV-EVA1ext-hFcIgG3-IRES-YFP

Plasmid

#64926

Purpose

To express extracellular portion of EVA1 (mouse) fused with human Fc(IgG3)

Depositor

Pau Serra

Article

Garabatos et al Monoclon Antib Immunodiagn Immunother. 2

Insert

mouse EVA1 fused with human FcIgG3 (Mpzl2, IGHG3 Mouse, Human)

Use

Retroviral

Tags

IRES-YFP

Expression

Mammalian

Promoter

LTR

Available Since

June 5, 2015

Availability

Academic Institutions and Nonprofits only
pSc2

Plasmid

#80437

Purpose

Expresses eGFP along with an U6 promoter driven gRNA which cleaves the plasmid in-cell

Depositor

Ervin Welker

Article

Talas et al DNA Res. 2017 Jun 30. doi: 10.1093/dnare

Inserts

sp Cas9 gRNA
eGFP

Use

CRISPR

Expression

Mammalian

Promoter

CMV and U6

Available Since

Sept. 12, 2016

Availability

Academic Institutions and Nonprofits only

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TRCN0000297275

Plasmid

#78152

Purpose

shCDK4 targeting 3UTR

Depositor

William Hahn

Article

Hong et al Nat Commun. 2016 Jun 22;7:11987. doi: 10

Insert

CDK4 (CDK4 Human)

Use

RNAi

Promoter

hU6

Available Since

June 22, 2016

Availability

Academic Institutions and Nonprofits only
TRCN0000279887

Plasmid

#78153

Purpose

shCDK4 targeting CDS

Depositor

William Hahn

Article

Hong et al Nat Commun. 2016 Jun 22;7:11987. doi: 10

Insert

CDK4 (CDK4 Human)

Use

Lentiviral and RNAi

Promoter

hU6 and hU6

Available Since

June 22, 2016

Availability

Academic Institutions and Nonprofits only
TRCN0000279952

Plasmid

#78155

Purpose

shCDK4 targeting 3UTR

Depositor

William Hahn

Article

Hong et al Nat Commun. 2016 Jun 22;7:11987. doi: 10

Insert

CDK4 (CDK4 Human)

Use

Lentiviral and RNAi

Promoter

hU6 and hU6

Available Since

June 22, 2016

Availability

Academic Institutions and Nonprofits only
TRCN0000279953

Plasmid

#78154

Purpose

shCDK4 targeting CDS

Depositor

William Hahn

Article

Hong et al Nat Commun. 2016 Jun 22;7:11987. doi: 10

Insert

CDK4 (CDK4 Human)

Use

Lentiviral and RNAi

Promoter

hU6 and hU6

Available Since

June 22, 2016

Availability

Academic Institutions and Nonprofits only
BPK1520-sgRNA GLB1

Plasmid

#184378

Purpose

Expresses a sgRNA to edit GLB1 gene NM_000404.2_c.907A>G

Depositor

David Gilot

Article

Leclerc et al CRISPR J. 2023 Feb;6(1):17-31. doi: 10.1

Insert

sgRNA targeting GLB1 (GLB1 Human)

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

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EF1a-FXN-P2A-mRuby2(miRE.FF4)-TS.FF6x1-bGH

Plasmid

#235301

Purpose

ComMAND open-loop circuit regulating FXN-P2A-mRuby2 (therapeutically relevant gene)

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

FXN-P2A-mRuby2 (FXN Human)

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF1a (human EF1a)

Available Since

June 11, 2025

Availability

Academic Institutions and Nonprofits only

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EF1a-FXN-P2A-mRuby2-bGH

Plasmid

#235300

Purpose

ComMAND base gene regulating FXN-P2A-mRuby2 (therapeutically relevant gene)

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

FXN-P2A-mRuby2 (FXN Human)

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF1a (human EF1a)

Available Since

May 30, 2025

Availability

Academic Institutions and Nonprofits only

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EF1a-FXN-P2A-mRuby2(miRE.FF4)-TS.FF4x1-bGH

Plasmid

#235302

Purpose

ComMAND closed-loop circuit regulating FXN-P2A-mRuby2 (therapeutically relevant gene)

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

FXN-P2A-mRuby2 (FXN Human)

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF1a (human EF1a)

Available Since

May 30, 2025

Availability

Academic Institutions and Nonprofits only

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Rab11 single guide RNA

Plasmid

#229683

Purpose

guide RNA for Rab11 tagging at the N-terminus in pX459

Depositor

Francesca Bottanelli

Article

Stockhammer et al Nat Cell Biol. 2024 Oct 4. doi: 10.1038/

Insert

Rab11 guide (RAB11A Human)

Use

CRISPR

Available Since

Jan. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pDR111_mannose_Stat1Klf6

Plasmid

#188400

Purpose

Expresses mouse transcription factors Stat-1 and Klf6 in an operon from D-mannose promoter for Bacillus subtilis. The secretion peptide PhoD is fused to both factors.

Depositor

Christopher Contag

Article

Madsen et al Commun Biol. 2022 Aug 30;5(1):888. doi:

Inserts

Stat-1 (Stat1 Escherichia coli, Mouse)
Klf6 (Klf6 Mouse)

Tags

PhoD secretion peptide

Expression

Bacterial

Promoter

D-mannose

Available Since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pBoBi-hPEN2 D90A

Plasmid

#183641

Purpose

Lentiviral expression of a PEN2 mutant losing its binding affinity to metformin at the C-terminus (lysosome lumen).

Depositor

Sheng-cai Lin

Article

Ma et al Nature. 2022 Mar;603(7899):159-165. doi:

Insert

PEN2 (PSENEN Human)

Use

Lentiviral

Tags

HA

Mutation

residues 90 of PEN2 mutated to alanine

Promoter

CMV

Available Since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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pBoBi-hPEN2 2A

Plasmid

#183640

Purpose

Lentiviral expression of a PEN2 mutant losing its binding affinity to metformin at the N-terminus (cytosolic face).

Depositor

Sheng-cai Lin

Article

Ma et al Nature. 2022 Mar;603(7899):159-165. doi:

Insert

PEN2 (PSENEN Human)

Use

Lentiviral

Tags

HA

Mutation

residues 35 and 40 of PEN2 mutated to alanine

Promoter

CMV

Available Since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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PiggyBac CAG eGFP-PolQ-FLAG P2A-BLAST

Plasmid

#249811

Purpose

Expresses the PolQ (polymerase theta) protein fused with N-terminal eGFP and C-terminal FLAG tag

Depositor

Raphael Ceccaldi

Article

Gelot et al Nature. 2023 Sep;621(7978):415-422. doi:

Insert

Polymerase Theta (POLQ Human)

Tags

FLAG and eGFP

Expression

Mammalian

Promoter

CAG

Available Since

March 6, 2026

Availability

Academic Institutions and Nonprofits only

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PB-CAG-mRuby3-Gal8-P2A-Zeo

Plasmid

#150815

Purpose

Mammalian expression of mRuby3-Galectin8 (Gal8) fusion protein

Depositor

Jordan Green

Article

Rui et al Sci Adv. 2022 Jan 7;8(1):eabk2855. doi:

Insert

mRuby3-Gal8-P2A-Zeo (LGALS8 Synthetic, Human)

Tags

Gal8 is fused to the c-terminus of mRuby3

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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Venus1-WTSTAT3

Plasmid

#123164

Purpose

Expresses wild type STAT3 fused to the 1-157 fragment of Venus in mammalian cells

Depositor

Federico Herrera

Article

Letra-Vilela et al FASEB Bioadv. 2020 Jan 27;2(2):116-125.

Insert

STAT3 tagged with Venus1 (aa 1-157) (STAT3 Human)

Tags

Venus1 (aa 1-158) for bimolecular fluorescence co…

Expression

Mammalian

Mutation

none

Promoter

pCMV

Available Since

Aug. 28, 2020

Availability

Academic Institutions and Nonprofits only

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AP1muA-Halo-ALFA-polyA-G418 HR

Plasmid

#229681

Purpose

Homology repair plasmid for endogenous tagging of AP1muA at the C-terminus with HaloTag and an ALFA tag. Contains a G418 resistance cassette for selection of edited cells.

Depositor

Francesca Bottanelli

Article

Stockhammer et al Nat Cell Biol. 2024 Oct 4. doi: 10.1038/

Insert

genomic sequence AP1M1 locus (AP1M1 Human)

Use

CRISPR

Tags

Halo, ALFA

Available Since

Jan. 3, 2025

Availability

Academic Institutions and Nonprofits only

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loxP-G418-loxP-3xALFA-Halo-Rab11 HR

Plasmid

#229676

Purpose

Homology repair plasmid for endogenous tagging of Rab11 at the N-terminus with Halotag and 3x ALFA tag. Contains a G418 resistance cassette for selection of edited cells

Depositor

Francesca Bottanelli

Article

Stockhammer et al Nat Cell Biol. 2024 Oct 4. doi: 10.1038/

Insert

genomic sequence Rab11 locus (RAB11A Human)

Use

CRISPR

Tags

Halo, 3xALFA

Available Since

Jan. 3, 2025

Availability

Academic Institutions and Nonprofits only

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