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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 14,321 results for: cas9

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Showing: 7521 - 7540 of 14321 results

  4. HCP8

    Plasmid
    #166110
    Purpose
    This plasmid encodes a Cas9 protein as well as a sgRNA that targets the C-terminus of Whi5
    Depositor
    Matthias Heinemann
    Insert
    Whi5-sg106 (WHI5 Budding Yeast)
    Use
    CRISPR
    Expression
    Yeast
    Available since
    April 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    166110-plasmid-map-sequence-id-330382

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  5. HCP5

    Plasmid
    #166107
    Purpose
    This plasmid encodes a Cas9 protein as well as a sgRNAs that targets the C-terminus of Cyr1.
    Depositor
    Matthias Heinemann
    Insert
    Cyr1CT-sgRNA (CYR1 Budding Yeast)
    Use
    CRISPR
    Expression
    Yeast
    Available since
    April 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    166107-plasmid-map-sequence-id-330320

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  6. pCMV-T7-TadCBEd-SpRY-P2A-EGFP (BKS428)

    Plasmid
    #223125
    Purpose
    pCMV and pT7 Human expression plasmid for TadCBEd-SpRY with C-term BPNLS-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Publication
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    human codon optimized TadCBEd-SpRY-2xUGI-P2A-EGFP
    Use
    CRISPR
    Tags
    2xUGI-BPNLS-P2A-EGFP and BPNLS-TadA-CDd
    Expression
    Mammalian
    Mutation
    TadA-CDd mutations; nSpRY=D10A/A61R/L1111R/D1135L…
    Promoter
    CMV and T7
    Available since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223125-plasmid-map-sequence-id-447703

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  14. pHelper-CRISPRi-sgRNA

    Plasmid
    #221135
    Purpose
    Helper plasmid for sgRNA cloning for CRISPRi. sgRNA-scaffold with dCas9 handle expressed from constitutive bacterial promoter J23119(SpeI). 2 BbsI sites for sgRNA cloning.
    Depositor
    Craig Roy
    Publication
    Steiner et al mSphere. 2024 Nov 19:e0052324. doi: 10.1
    Insert
    sgRNA-scaffold with dCas9 handle
    Use
    CRISPR
    Expression
    Bacterial
    Promoter
    J23119(SpeI)
    Available since
    Nov. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    221135-plasmid-map-sequence-id-436077

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  16. pMLS716

    Plasmid
    #188888
    Purpose
    Targeting vector for insertion into 5' miniMos Arm; carries floxed Cbr-unc-119 marker and Phsp16.41::Cre
    Depositor
    Erik Jorgensen
    Publication
    Schwartz et al PLoS Genet. 2021 Nov 8;17(11):e1009755.
    Insert
    floxed Cbr-unc-119 marker and Phsp16.41::Cre
    Expression
    Worm
    Mutation
    C. elegans codom optimization and intron addition
    Available since
    Aug. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    188888-plasmid-map-sequence-id-370784

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Showing: 7521 - 7540 of 14321 results