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Pzac2.1 U6-Ezr sgRNA1, 2, 3_gfaABC1D mcherry SV40

Plasmid

#179119

Purpose

Expresses Ezr sgRNAs under the U6 promoter and mcherry protein specifically in astrocytes

Depositor

Baljit Khakh

Article

Endo et al Science. 2022 Nov 4;378(6619):eadc9020.

Insert

Ezr sgRNA

Use

AAV

Promoter

U6

Available Since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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MPRA Empty Vector

Plasmid

#204865

Purpose

Vector used for high-throughput cloning and subsequent transfection of MPRA libraries into mammalian cells.

Depositor

Philipp Maass

Article

Oliveros et al Cell Genom. 2023 May 24;3(7):100330. doi

Type

Empty backbone

Expression

Bacterial and Mammalian

Available Since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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HA_Axin1 in pRK5

Plasmid

#154219

Purpose

N-terminal HA-tagged AXIN1 cDNA expression vector for transfections

Depositor

David Sabatini

Article

Orozco et al Nat Metab. 2020 Sep;2(9):893-901. doi: 1

Insert

AXIN1 (AXIN1 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV_SynP_DIO_tAKARα- T391A_WPRE

Plasmid

#196676

Purpose

AAV plasmid for mammalian Cre-dependent expression of the T391A mutant of tAKARα (abolished sensor phosphorylation site).

Depositor

Haining Zhong

Article

Ma et al Nature. 2022 Nov;611(7937):762-768. doi:

Insert

tAKARα-T391A

Use

AAV and Cre/Lox

Expression

Mammalian

Available Since

Feb. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-hSynI-fDIO(TagBFP)-KoNLS-Cre(MtoL)

Plasmid

#180587

Purpose

FdCd

Depositor

Won Do Heo

Article

Jung et al Nat Commun. 2019 Jan 18;10(1):314. doi:

Insert

Cre

Use

AAV

Available Since

Feb. 28, 2022

Availability

Academic Institutions and Nonprofits only

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PRS_8-eGFP

Plasmid

#192589

Purpose

AAV-vector for fluorescent labeling of noradrenergic neurons

Depositor

Simon Wiegert

Article

Wissing et al PLoS Biol. 2025 Jul 7;23(7):e3003228. do

Insert

EGFP

Use

AAV

Expression

Mammalian

Promoter

PRSX8

Available Since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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AAV-CMV-eGFP

Plasmid

#193022

Purpose

AAV vector plasmid expressing enhanced green fluorescent protein (eGFP) under the cytomegalovirus (CMV) promoter

Depositor

Bart Nieuwenhuis

Article

Nieuwenhuis et al Gene Ther. 2023 Jan 13. doi: 10.1038/s41

Insert

eGFP

Use

AAV

Expression

Mammalian

Promoter

CMV

Available Since

April 20, 2023

Availability

Academic Institutions and Nonprofits only

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CaMKIIP-mEGFP-P2A-paAIP2 (R5AR6A)/pAAV

Plasmid

#91719

Purpose

AAV-mediated expression of mEGFP and paAIP2 (R5AR6A), the functional mutant of paAIP2

Depositor

Ryohei Yasuda

Article

Murakoshi et al Neuron. 2017 Apr 5;94(1):37-47.e5. doi:

Insert

mEGFP-P2A-paAIP2(R5AR6A)

Use

AAV

Promoter

CaMKII 1.3

Available Since

June 2, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-pAceR

Plasmid

#195521

Purpose

Red fluorescent, positive response-polarity voltage indicator under the control of synapsin promoter

Depositor

Vincent Pieribone

Article

Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

Insert

pAceR

Use

AAV

Expression

Mammalian

Mutation

VARNAM 78E, 81D, 92N; SI linker

Promoter

Synapsin

Available Since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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AAV-hSYN-eGFP

Plasmid

#162512

Purpose

AAV vector plasmid expressing enhanced green fluorescent protein (eGFP) under the human synapsin (hSYN) promoter

Depositor

Bart Nieuwenhuis

Article

Nieuwenhuis et al Gene Ther. 2020 Jun 23. pii: 10.1038/s41

Insert

eGFP

Use

AAV

Expression

Mammalian

Promoter

hSYN

Available Since

April 20, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV.hSynap.(cyto).iATPSnFR2.A95K.tdTomato

Plasmid

#244921

Purpose

iATPSnFR2.A95K variant with non-responsive tdTomato

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

iATPSnFR2.A95K.tdTomato

Use

AAV

Mutation

A95K

Available Since

Nov. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV2-SynTetOff-GFP

Plasmid

#190231

Purpose

AAV vector, high-level expression of EGFP in neuronal cells

Depositor

Hiroyuki Hioki

Article

Sohn et al PLoS One. 2017 Jan 6;12(1):e0169611. doi

Insert

EGFP

Use

AAV

Expression

Mammalian

Available Since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-TIWB-myrmScarlet-I-P2A-post-eGRASP

Plasmid

#111584

Purpose

An AAV vector that expresses myristoylated mScarlet-I and post-eGRASP linked by self-cleaving P2A peptide under the tetO promoter.

Depositor

Bong-Kiun Kaang

Article

Choi et al Science. 2018 Apr 27;360(6387):430-435.

Insert

myrmScarlet-I-P2A-post-eGRASP

Use

AAV

Promoter

tetO

Available Since

June 12, 2018

Availability

Academic Institutions and Nonprofits only

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pTP288

Plasmid

#113460

Purpose

human Flag tagged Nbs1 in baculovirus transfer vector

Depositor

Tanya Paull

Article

Lee et al J Biol Chem. 2013 May 3;288(18):12840-51

Insert

Nbs1 (NBN Human)

Tags

Flag

Expression

Insect

Promoter

polyhedrin

Available Since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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plRL58

Plasmid

#166886

Purpose

Optimized M. tuberculosis CRISPRi plasmid; contains an L5 attP region (no L5 Integrase) that allows for integration when co-transformed with an L5 Integrase delivery plasmid (e.g. plRL19, ID #163634)

Depositor

Jeremy Rock

Article

Bosch et al Cell. 2021 Jul 16. pii: S0092-8674(21)00

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

Tet-On

Available Since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV hSyn:FLEX-Axon-jGCaMP8s

Plasmid

#239672

Purpose

Cre-dependent expression of axon targeted jGCaMP8s under hSyn promoter.

Depositor

Marianne Fyhn

Article

Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

Insert

Axon-jGCaMP8s

Use

AAV

Tags

GAP43Pal

Expression

Mammalian

Promoter

hSyn

Available Since

July 22, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-mCherry.3xFLAG-WPRE

Plasmid

#127861

Purpose

pAAV plasmid expressing an mCherry.3xFLAG fusion protein under the hSyn promoter

Depositor

Florian Freudenberg

Article

Candemir et al Eur Neuropsychopharmacol. 2016 Apr;26(4)

Insert

mCherry

Use

AAV

Tags

3xFLAG

Promoter

hSyn

Available Since

Aug. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pPRIME-TET-GFP-FF3

Plasmid

#11662

Purpose

3rd generation lentiviral transfer vector. pPRIME cloning plasmid with a tetracycline-responsive promoter (TET) controlling expression of GFP and miR30-based shRNA targeting firefly luciferase

Depositor

Stephen Elledge

Article

Stegmeier et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1

Insert

FF3

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Available Since

March 1, 2007

Availability

Academic Institutions and Nonprofits only

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pZac2.1 GfaABC1D rM3D mCherry SV40

Plasmid

#92285

Purpose

AAV DREADD expression

Depositor

Baljit Khakh

Article

Chai et al Neuron. 2017 Aug 2;95(3):531-549.e9. doi

Insert

rM3D

Use

AAV

Tags

mCherry

Promoter

GfaABC1D

Available Since

June 26, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV CaMKII:RiboL1-jGCaMP8s

Plasmid

#239667

Purpose

Expresses soma-targeted (ribosome tethered via RiboL1) jGCaMP8s under CaMKII promoter.

Depositor

Marianne Fyhn

Article

Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

Insert

RiboL1-jGCaMP8s

Use

AAV

Tags

6xHis and RiboL1

Expression

Mammalian

Promoter

CaMKII

Available Since

July 22, 2025

Availability

Academic Institutions and Nonprofits only

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