We narrowed to 9,647 results for: bli

1. pGEX-4T1-GST-Thrombin-TEV-EGFP-hNEMO

   Plasmid

   #199781

   Purpose

   Plasmid for the expression of GFP labelled NEMO (SMC Internal No. 1977).

   Depositor

   Sascha Martens

   Insert

   NEMO (IKBKG Human)
   

   Tags

   GST-Thrombin-TEV-EGFP

   Expression

   Bacterial

   Available Since

   May 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. 7MEQ

   Plasmid

   #176412

   Purpose

   Baculovirus expression for structure determination. May not contain entire coding region of gene.

   Depositor

   Cheryl Arrowsmith

   Insert

   TMPRSS2 – transmembrane serine protease 2 (TMPRSS2 Human)
   

   Use

   Baculovirus expresssion

   Tags

   8 x His Tag and Honeybee melittin signal

   Mutation

   etmprss2_S251D/R252D/Q253D/S254D/R255K

   Promoter

   Polyhedrin

   Available Since

   Oct. 21, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. TAF1

   Plasmid

   #39117

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   TAF1 (TAF1 Human)
   

   Tags

   His Tev

   Expression

   Bacterial

   Available Since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

4. Lenti-DAG1

   Plasmid

   #205149

   Purpose

   Overexpress DAG1

   Depositor

   Monkol Lek

   Insert

   Dystroglycan 1 (DAG1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   Carries common missense variant c.41C>G (p.Ser…

   Promoter

   EF-1a

   Available Since

   April 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-hSyn-DIO-HA-hM4D(Gi)-IRES-mCitrine

   Plasmid

   #50455

   Purpose

   Double floxed Gi-coupled hM4D-IRES-mCitrine under the control of human synapsin promoter

   Depositor

   Bryan Roth

   Has Service

   AAV8

   Insert

   hM4D(Gi)-IRES-mCitrine (CHRM4 Human)
   

   Use

   AAV

   Tags

   HA

   Mutation

   See supplemental documents for DREADD mutations

   Promoter

   human Synapsin 1

   Available Since

   April 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFP.E227

   Plasmid

   #138249

   Purpose

   Reporter plasmid - Expresses three fluorescent reporters from three different ECF promoters

   Depositor

   Baojun Wang

   Article

   Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

   Inserts

   mCherry
   Terminator
   mTagBFP
   Terminator
   gfp
   Terminator
   

   Use

   Synthetic Biology

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   P17_up1691, P20_992, and iP16_3622

   Available Since

   March 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHR_5xUAS-ETV2-P2A-BFP-WPRE_PGK-HygR

   Plasmid

   #216664

   Purpose

   Expressed Gal4-inducible ETV2 and BFP, with hygromycin resistance

   Depositor

   Leonardo Morsut

   Inserts

   ETV2 (Etv2 Mouse)
   BFP
   HygromycinR
   

   Use

   Lentiviral

   Available Since

   March 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCDB179-CaPETaseM9

   Plasmid

   #218707

   Purpose

   Encodes codon optimized CaPETaseM9 enzyme for bacterial expression

   Depositor

   Gregg Beckham

   Article

   Norton-Baker et al Sci Rep. 2024 Jun 24;14(1):14449. doi: 1

   Insert

   CaPETaseM9
   

   Tags

   10xHis

   Expression

   Bacterial

   Available Since

   May 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pOTTC1740 - pAAV SYN1 DIO HA-hM4D(Gi)

   Plasmid

   #125146

   Purpose

   An adeno-associated viral vector expressing Cre-dependent HA-tagged inhibitory DREADD (hM4D(Gi)) from a synapsin promoter

   Depositor

   Christopher Richie

   Insert

   HA-tagged hM4D(Gi) (CHRM4 Human)
   

   Use

   AAV and Cre/Lox

   Tags

   HA

   Expression

   Mammalian

   Promoter

   SYN1

   Available Since

   May 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. AICSDP-166:CDH1-mEGFP

    Plasmid

    #193921

    Purpose

    Homology arms and LE-mEGFP sequence for C-terminus tagging of human cadherin 1

    Depositor

    Allen Institute for Cell Science

    Insert

    cadherin 1 Homology Arms with LE-mEGFP (CDH1 Human)
    

    Use

    AAV and CRISPR; Donor template

    Tags

    linker-mEGFP

    Mutation

    homology arms contain point mutations to disrupt …

    Available Since

    Dec. 22, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pET29b Tau Cys

    Plasmid

    #108867

    Purpose

    Expresses human Tau with a mutated cysteine

    Depositor

    Gabriele Kaminski Schierle

    Insert

    Tau (MAPT Human)
    

    Expression

    Bacterial

    Mutation

    I260C C291A C322A to allow maleimide dye labelling

    Promoter

    T7

    Available Since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRN3P_membrane_EGFP

    Plasmid

    #139402

    Purpose

    in vitro transcription for mRNA

    Depositor

    Maria-Elena Torres-Padilla

    Article

    Borsos et al Nature. 2019 May;569(7758):729-733. doi:

    Insert

    membrane-EGFP
    

    Use

    In vitro transcription

    Tags

    EGFP

    Promoter

    T3

    Available Since

    March 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. AICSDP-60: SOX2-mEGFP

    Plasmid

    #124606

    Purpose

    Homology arms and linker-mEGFP sequence for C-terminus tagging of human SOX2

    Depositor

    Allen Institute for Cell Science

    Insert

    SOX2 Homology Arms with linker-mEGFP (SOX2 Human)
    

    Use

    CRISPR; Donor template

    Expression

    Mammalian

    Available Since

    May 13, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pTipi2.1_Anti-ProC [IPI-HPC-4] Mouse/Rabbit HC

    Plasmid

    #221361

    Purpose

    Mammalian expression of Anti-ProC [IPI-HPC-4] Mouse/Rabbit HC; to be used with Anti-ProC [IPI-HPC-4] light chain (Plasmid 221362) to make the antibody.

    Depositor

    Institute for Protein Innovation (IPI)

    Insert

    Anti-ProC [IPI-HPC-4] Mouse/Rabbit HC
    

    Use

    Affinity Reagent/ Antibody

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 1, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pBABE.puro/TO/Flag/tGFP-HuR.wt

    Plasmid

    #110349

    Purpose

    Doxycycline inducible mammalian retroviral expression of HuR (wild type) with FLAG and turboGFP tags

    Depositor

    Sandra Martha Gomes Dias

    Insert

    ELAV like RNA binding protein 1 (ELAVL1 Human)
    

    Use

    Retroviral

    Tags

    FLAG and tGFP

    Expression

    Mammalian

    Promoter

    gag

    Available Since

    Nov. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. DT0

    Plasmid

    #80418

    Purpose

    Expresses 0 CUG repeats in the context of human DMPK genomic segment containing exons 11-15 with repeats inserted at site in exon 15 that contains the repeats

    Depositor

    Thomas Cooper

    Article

    Ho et al EMBO J. 2004 Aug 4;23(15):3103-12. Epub

    Insert

    human DMPK exons 11-15 with 0 CTG repeats (DMPK Human)
    

    Expression

    Mammalian

    Available Since

    Sept. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. Membrane mNeonGreen

    Plasmid

    #198057

    Purpose

    Plasma membrane localizing mNeonGreen using Human LCK proto-oncogene sequence

    Depositor

    Amro Hamdoun

    Insert

    LCK membrane mNeonGreen (LCK Human, A.victoria)
    

    Use

    Synthetic Biology; Sea urchin, mammal, zebrafish

    Tags

    mNeonGreen

    Mutation

    Tandem repeat of N-terminal LCK membrane targetin…

    Promoter

    sp6

    Available Since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. Lenti_AID-Dam-only

    Plasmid

    #119765

    Purpose

    Lentiviral plasmid for Mammalian Expression

    Depositor

    Maria-Elena Torres-Padilla

    Article

    Borsos et al Nature. 2019 May;569(7758):729-733. doi:

    Insert

    AID-Dam only
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Nov. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pOD_009

    Plasmid

    #159520

    Purpose

    Rifampicin version of the scarless genome engineering pDEL plasmid (Tikh et al. 2016) compatible with Salmonella

    Depositor

    Elisabeth Raleigh

    Article

    Zaworski et al PLoS Genet. 2022 Apr 4;18(4):e1009943. d

    Type

    Empty backbone

    Use

    Synthetic Biology

    Available Since

    Sept. 30, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pTipi2.1_Anti-EE [IPI-3D5EE_48.K] Mouse/Rabbit LC kappa

    Plasmid

    #221352

    Purpose

    Mammalian expression of Anti-EE [IPI-3D5EE_48.K] Mouse/Rabbit LC kappa; to be used with Anti-EE [IPI-3D5EE_48.K] heavy chain (Plasmid 221351) to make the antibody.

    Depositor

    Institute for Protein Innovation (IPI)

    Insert

    Anti-EE [IPI-3D5EE_48.K] Mouse/Rabbit LC kappa
    

    Use

    Affinity Reagent/ Antibody

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 1, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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