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p-mCherry-C1-MerMAID3

Plasmid

#126515

Purpose

Anion-conducting Channelrhodopsin with near-complete desensitization in continuous light. Codon-optimized for mammalian expression (human/mouse).

Depositor

Peter Hegemann

Article

Oppermann et al Nat Commun. 2019 Jul 25;10(1):3315. doi:

Insert

MerMAID3

Tags

mCherry

Expression

Mammalian

Promoter

CMV (+ enhancer)

Available Since

May 22, 2019

Availability

Academic Institutions and Nonprofits only

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p-mCherry-C1-MerMAID6

Plasmid

#126518

Purpose

Anion-conducting Channelrhodopsin with near-complete desensitization in continuous light. Codon-optimized for mammalian expression (human/mouse).

Depositor

Peter Hegemann

Article

Oppermann et al Nat Commun. 2019 Jul 25;10(1):3315. doi:

Insert

MerMAID6

Tags

mCherry

Expression

Mammalian

Promoter

CMV (+ enhancer)

Available Since

May 22, 2019

Availability

Academic Institutions and Nonprofits only

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p-mCherry-C1-MerMAID4

Plasmid

#126516

Purpose

Anion-conducting Channelrhodopsin with near-complete desensitization in continuous light. Codon-optimized for mammalian expression (human/mouse).

Depositor

Peter Hegemann

Article

Oppermann et al Nat Commun. 2019 Jul 25;10(1):3315. doi:

Insert

MerMAID4

Tags

mCherry

Expression

Mammalian

Promoter

CMV (+ enhancer)

Available Since

May 22, 2019

Availability

Academic Institutions and Nonprofits only

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p-mCherry-C1-MerMAID7

Plasmid

#126519

Purpose

Anion-conducting Channelrhodopsin with near-complete desensitization in continuous light. Codon-optimized for mammalian expression (human/mouse).

Depositor

Peter Hegemann

Article

Oppermann et al Nat Commun. 2019 Jul 25;10(1):3315. doi:

Insert

MerMAID7

Tags

mCherry

Expression

Mammalian

Promoter

CMV (+ enhancer)

Available Since

May 22, 2019

Availability

Academic Institutions and Nonprofits only

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p-mCherry-C1-MerMAID1

Plasmid

#126513

Purpose

Anion-conducting Channelrhodopsin with near-complete desensitization in continuous light. Codon-optimized for mammalian expression (human/mouse).

Depositor

Peter Hegemann

Article

Oppermann et al Nat Commun. 2019 Jul 25;10(1):3315. doi:

Insert

MerMAID1

Tags

mCherry

Expression

Mammalian

Promoter

CMV (+ enhancer)

Available Since

May 22, 2019

Availability

Academic Institutions and Nonprofits only

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AF10_OM-LZ

Plasmid

#111753

Purpose

Bacterial expression for structure determination

Depositor

Cheryl Arrowsmith

Article

Zhang et al Genes Dev. 2018 Mar 1;32(5-6):341-346. d

Insert

MLLT10 (MLLT10 Human)

Tags

6x His - TEV

Expression

Bacterial

Mutation

Contains the OM-LZ region of AF10 (residues 720-7…

Promoter

T7

Available Since

June 14, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pKK-TEV-mCardinal

Plasmid

#105798

Purpose

Expression of your protein of interest in fusion with red fluorescent protein at the C-terminus (cleavable by TEV), (PMID: 24633408). mCardinal is suitable for deep-tissue imaging.

Depositor

Andrzej Dziembowski

Article

Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

Type

Empty backbone

Use

Flp-in competent

Tags

TEV-mCardinal

Expression

Mammalian

Available Since

Feb. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pKK-TEV-mCerulean

Plasmid

#105790

Purpose

Expression of your protein of interest in fusion with cyan fluorescent protein at the C-terminus (cleavable by TEV). Useful for FRET experiments (PMID: 14990965, 17040988, 22264545).

Depositor

Andrzej Dziembowski

Article

Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

Type

Empty backbone

Use

Flp-in competent

Tags

TEV-mCerulean

Expression

Mammalian

Available Since

Feb. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pKK-mCardinal-TEV

Plasmid

#105781

Purpose

Expression of your protein of interest in fusion with red fluorescent protein at the N-terminus (cleavable by TEV), (PMID: 24633408). mCardinal is suitable for deep-tissue imaging.

Depositor

Andrzej Dziembowski

Article

Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

Type

Empty backbone

Use

Flp-in competent

Tags

mCardinal-TEV

Expression

Mammalian

Available Since

Feb. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pKK-mCerulean-TEV

Plasmid

#105773

Purpose

Expression of your protein of interest in fusion with cyan fluorescent protein at the N-terminus (cleavable by TEV). Useful for FRET experiments (PMID: 14990965, 17040988, 22264545).

Depositor

Andrzej Dziembowski

Article

Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

Type

Empty backbone

Use

Flp-in competent

Tags

mCerulean-TEV

Expression

Mammalian

Available Since

Feb. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pFastBac1-OSF-TRIM5alpha(African green monkey pygerythrus)(1-293)

Plasmid

#79030

Purpose

Baculoviral entry vector to produce Bac-to-bac baculoviruses

Depositor

Wesley Sundquist

Article

Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

Insert

OSF-TRIM5alpha(African green monkey pygerythrus)(1-293)

Tags

OneSTrEP-FLAG

Expression

Insect

Mutation

deleted amino acid 294-515 from frican green monk…

Promoter

polyhedrin

Available Since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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KC1G3_HUMAN_D0

Plasmid

#79685

Purpose

This plasmid encodes the kinase domain of KC1G3. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

KC1G3 (CSNK1G3 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
pQCXIX_sh_hMIB2_#9

Plasmid

#75255

Purpose

retroviral shRNA vector against human MIB2, expresses GFP for transduction control

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

shRNA against MIB2 (human) (MIB2 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Promoter

mU6

Available Since

June 20, 2016

Availability

Academic Institutions and Nonprofits only

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pZS1[GalS-L,RbsR-L]

Plasmid

#60763

Purpose

Contains PIq driving expression GalS-L, and PIq driving expression of RbsR-L.

Depositor

Matthew Bennett

Article

Shis et al ACS Synth Biol. 2014 Sep 19;3(9):645-51.

Inserts

GalS-L
RbsR-L

Use

Synthetic Biology

Expression

Bacterial

Mutation

LacI/GalR repressor chimera. LacI DBD with GalS L…

Available Since

July 10, 2015

Availability

Academic Institutions and Nonprofits only

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pZS1[GalS-L,FruR-L]

Plasmid

#60764

Purpose

Contains PIq driving expression GalS-L, and PIq driving expression of FruR-L.

Depositor

Matthew Bennett

Article

Shis et al ACS Synth Biol. 2014 Sep 19;3(9):645-51.

Inserts

GalS-L
FruR-L

Use

Synthetic Biology

Expression

Bacterial

Mutation

LacI/GalR repressor chimera. LacI DBD with FruR L…

Available Since

July 10, 2015

Availability

Academic Institutions and Nonprofits only

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pZS1[GalS-L,TreR-L]

Plasmid

#60765

Purpose

Contains PIq driving expression GalS-L, and PI driving expression of TreR-L.

Depositor

Matthew Bennett

Article

Shis et al ACS Synth Biol. 2014 Sep 19;3(9):645-51.

Inserts

GalS-L
TreR-L

Use

Synthetic Biology

Expression

Bacterial

Mutation

LacI/GalR repressor chimera. LacI DBD with GalS L…

Available Since

July 10, 2015

Availability

Academic Institutions and Nonprofits only

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pZS1[FruR-L,RbsR-L]

Plasmid

#60761

Purpose

Contains PIq driving expression TreR-L, and PIq driving expression of RbsR-L

Depositor

Matthew Bennett

Article

Shis et al ACS Synth Biol. 2014 Sep 19;3(9):645-51.

Inserts

FruR-L
RbsR-L

Use

Synthetic Biology

Expression

Bacterial

Mutation

LacI/GalR repressor chimera. LacI DBD with FruR L…

Available Since

July 10, 2015

Availability

Academic Institutions and Nonprofits only

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pZS1[RbsR-L,TreR-L]

Plasmid

#60766

Purpose

Contains PIq driving expression RbsR-L, and PI driving expression of TreR-L.

Depositor

Matthew Bennett

Article

Shis et al ACS Synth Biol. 2014 Sep 19;3(9):645-51.

Inserts

RbsR-L
TreR-L

Use

Synthetic Biology

Expression

Bacterial

Mutation

LacI/GalR repressor chimera. LacI DBD with RbsR L…

Available Since

April 28, 2015

Availability

Academic Institutions and Nonprofits only

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TriEx-4 DHX36

Plasmid

#68368

Purpose

Expresses the N-terminal histidine-tagged DXH36 gene product upon transfection into Rosetta 2 E. coli and induction with IPTG.

Depositor

James Vaughn

Article

Vaughn et al J Biol Chem. 2005 Nov 18;280(46):38117-2

Insert

DHX36 (DHX36 Human)

Tags

Histidine

Expression

Bacterial, Insect, and Mamm…

Promoter

CMV ie enhancer/promoter

Available Since

Sept. 29, 2016

Availability

Academic Institutions and Nonprofits only
pCMV-bpNLS-eeBxb1 (CJT555)

Plasmid

#248209

Purpose

eeBxb1 recombinase with N-terminal bpNLS, expressed from a CMV promoter.

Depositor

Benjamin Kleinstiver

Article

Tou et al Nature. 2026 Mar 11. doi: 10.1038/s41586

Insert

bpNLS-eeBxb1

Use

CRISPR; In vitro transcription

Tags

BPNLS

Expression

Mammalian

Mutation

eeBxb1(V74A, E229K, V375I)

Promoter

CMV and T7

Available Since

April 15, 2026

Availability

Academic Institutions and Nonprofits only

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