We narrowed to 24,987 results for: Nov;
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Plasmid#228507PurposeBacterial Hybrid Driver PlasmidDepositorInsertT7 RNAP, LARP-I
UseSynthetic BiologyTagsUmu Degron, GTG-start codonMutationR378K; S430P, N433T, S633P, F849I, F880Y; W727G, …Available SinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEM_Δflv3.sm
Plasmid#185533Purposea pGEM-T easy vector containing the streptomycin/spectinomycin resistance cassette flanked by the two regions for the double homologous recombination on the flv3 locusDepositorInsertaadA gene flanked by flv3 upstream and downstream regions
UseSynthetic BiologyAvailable SinceOct. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pACYC-yhaM
Plasmid#207650PurposeOverexpression of native L-cysteine desulfidase in E. coli MG1655DepositorInsertyhaM
Tags6xHisExpressionBacterialPromoterT7Available SinceOct. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pACYC-decR
Plasmid#207653PurposeOverexpression of L-cysteine desulfidase transcriptional regulator in E. coli MG1655DepositorInsertdecR
ExpressionBacterialPromoterT7Available SinceOct. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMF2-yhaO
Plasmid#207654PurposeOverexpression of L-cysteine importer in E. coli MG1655DepositorInsertyhaO
Tags6xHisExpressionBacterialPromoteraraBpAvailable SinceOct. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
RT-4
Plasmid#220443PurposeConstruct used to generate the PUF3/PUP2/3HA tagging strains. Surrounding the PUP2/3HA/URA3 are large regions of homology to integrate at the 3’ end of the PUF3 gene.DepositorAvailable SinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28_Caspase-11_humanized-mutant
Plasmid#214315PurposeProtein overexpression in bacteriaDepositorInsertCASP4 (Casp4 Mouse)
TagsHis-TEVExpressionBacterialMutationN152K_KLS288YKT_KASIHS348TPRAKAPromoterT7Available SinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28_Caspase-4_W267N
Plasmid#214314PurposeProtein overexpression in bacteriaDepositorAvailable SinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28_Caspase-4_R269D
Plasmid#214313PurposeProtein overexpression in bacteriaDepositorAvailable SinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
LV-sgRNA-mCherry-Puro sgPIDD
Plasmid#211528PurposeDeletes PIDDDepositorInsertsgPIDD
UseCRISPR and LentiviralExpressionMammalianAvailable SinceFeb. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPR-v2 hygro sgUCK1
Plasmid#211524PurposeDeletes UCK1DepositorInsertsgUCK1
UseCRISPR and LentiviralExpressionMammalianAvailable SinceJan. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
TRAPa(R150A)-3xFLAG-pCDNA
Plasmid#201966PurposeMammalian expression of mutated Translocon-associated protein subunit alphaDepositorAvailable SinceJan. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-HsTNRC6C_1207-1609-M2WA_T
Plasmid#147634PurposeInsect Expression of HsTNRC6C_1207-1609-M2WADepositorInsertHsTNRC6C_1207-1609-M2WA (TNRC6C Human)
ExpressionInsectAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-HsTNRC6C_1207-1609_R
Plasmid#147410PurposeInsect Expression of HsTNRC6C_1207-1609DepositorInsertHsTNRC6C_1207-1609 (TNRC6C Human)
ExpressionInsectAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C2-tepsin
Plasmid#196707PurposeExpresses GFP-tepsin in mammalian cellsDepositorInserttepsin (TEPSIN Human)
TagsEGFPExpressionMammalianMutationsilent substitution in codon 199 to eliminate int…PromoterCMVAvailable SinceMarch 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAFMW-Nbr[C399-625]
Plasmid#188587PurposeFlag-tagged Nibbler variants expression in Drosophila S2 cellsDepositorInsertNibbler C-terminal domain
TagsFLAG-MycExpressionInsectAvailable SinceOct. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28b-bhaA
Plasmid#167810PurposeExpresses His6-tagged BhaA in E. coliDepositorInsertBhaA
TagsHis6ExpressionBacterialPromoterT7Available SinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28b-bhaB1
Plasmid#167811PurposeExpresses His6-tagged BhaB1 in E. coliDepositorInsertBhaB1
TagsHis6ExpressionBacterialPromoterT7Available SinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28b-bhaB7
Plasmid#167813PurposeExpresses His6-tagged BhaB7 in E. coliDepositorInsertBhaB7
TagsHis6ExpressionBacterialPromoterT7Available SinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET15b-bhaC1
Plasmid#167814PurposeExpresses BhaC1 in E. coliDepositorInsertBhaC1
ExpressionBacterialPromoterT7Available SinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
1137B=Hsp70Bb-Cas9
Plasmid#153284PurposeThe attB plasmid harboring the Hsp70Bb-Cas9-T2A-eGFP-p10 and a mini-white marker.DepositorInsertpHsp70Bb-SpCas9-T2A-eGFP-p10 (cas9 Synthetic)
UseCRISPRTagseGFPExpressionInsectPromoterDmel Hsp70BbAvailable SinceMarch 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
RIB ONLY R3
Plasmid#161511PurposeNon activation control for CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_only_SV40NLS_pGAP_RIB1_R3_gRNA_MS2DepositorInsertsdCAS9
MS2 (non activation control NAC)
gRNA (Targeting R3 in the RIB1 promoter)
ExpressionYeastPromoterpGAP, pPOR1, and pTEF2Available SinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
RIB ONLY R4
Plasmid#161512PurposeNon activation control for CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_only_SV40NLS_pGAP_RIB1_R4_gRNA_MS2DepositorInsertsdCAS9
MS2 (non activation control NAC)
gRNA (Targeting R4 in the RIB1 promoter)
ExpressionYeastPromoterpGAP, pPOR1, and pTEF2Available SinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
THI ONLY pALD4 T1
Plasmid#161498PurposeNon activation control for CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_only_SV40NLS_pALD4_THI11_T1_gRNA_MS2DepositorInsertsdCAS9
MS2 (non activation control NAC)
gRNA (Targeting T1 in the THI11 promoter)
ExpressionYeastPromoterpALD4, pPOR1, and pTEF2Available SinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
THI ONLY pALD4 T2
Plasmid#161499PurposeNon activation control for CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_only_SV40NLS_pALD4_THI11_T2_gRNA_MS2DepositorInsertsdCAS9
MS2 (non activation control NAC)
gRNA (Targeting T2 in the THI11 promoter)
ExpressionYeastPromoterpALD4, pPOR1, and pTEF2Available SinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
THI ONLY pALD4 T3
Plasmid#161500PurposeNon activation control for CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_only_SV40NLS_pALD4_THI11_T3_gRNA_MS2DepositorInsertsdCAS9
MS2 (non activation control NAC)
gRNA (Targeting T3 in the THI11 promoter)
ExpressionYeastPromoterpALD4, pPOR1, and pTEF2Available SinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
THI ONLY pALD4 T4
Plasmid#161501PurposeNon activation control for CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_only_SV40NLS_pALD4_THI11_T4_gRNA_MS2DepositorInsertsdCAS9
MS2 (non activation control NAC)
gRNA (Targeting T4 in the THI11 promoter)
ExpressionYeastPromoterpALD4, pPOR1, and pTEF2Available SinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
THI ONLY pALD4 T5
Plasmid#161502PurposeNon activation control for CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_only_SV40NLS_pALD4_THI11_T5_gRNA_MS2DepositorInsertsdCAS9
MS2 (non activation control NAC)
gRNA (Targeting T5 in the THI11 promoter)
ExpressionYeastPromoterpALD4, pPOR1, and pTEF2Available SinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
THI ONLY pGAP T-
Plasmid#161503PurposeNon activation control for CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_only_SV40NLS_pGAP_THI11_T-_gRNA_MS2DepositorInsertsdCAS9
MS2 (non activation control NAC)
gRNA (Non targeting control NTC)
ExpressionYeastPromoterpGAP, pPOR1, and pTEF2Available SinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
THI ONLY pGAP T1
Plasmid#161504PurposeNon activation control for CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_only_SV40NLS_pGAP_THI11_T1_gRNA_MS2DepositorInsertsdCAS9
MS2 (non activation control NAC)
gRNA (Targeting T1 in the THI11 promoter)
ExpressionYeastPromoterpGAP, pPOR1, and pTEF2Available SinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only