We narrowed to 9,887 results for: Coli
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Plasmid#132614Purposeexpresses human HUWE1 WWE (1611-1703) in E. coliDepositorInsertHUWE1 WWE (1611-1703)
ExpressionBacterialAvailable SinceOct. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFS_1064_pET30_pCat-tetR-Term-ptetA-FsRT-Cas1(opt)-Cas2(opt)-3xTerm_J23106-Leader-ARRAY2-DR2-FaqI_Term_NotI
Plasmid#184739Purposeencodes aTc inducible FsRT-Cas1–Cas2 expression cassette and FsCRISPR Array 2 transcribed by BBa_J23106DepositorInsertFsRT-Cas1(opt)-Cas2(opt)
ExpressionBacterialMutationsequence codon optimized for expression in E. coliPromoterpTetAAvailable SinceMay 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSR357.9
Plasmid#125105PurposeExpression of narL(1-129aa)-ydfI(124-213aa) chimera under PLtetO-1, output Promoter PydfJ115DepositorInsertsnarL(REC)-ydfI(DBD)129
sfgfp
tetR
UseSynthetic BiologyExpressionBacterialAvailable SinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGG426_UV5
Plasmid#165608PurposeVector for expression of the SpCas9 VRKG variant with sgRNA in E. coli: lacUV5-VRKG(SpCas9, D1135V/S1136R/D1332K/R1333G) and UV5-sgRNA (hEGFP spacer)DepositorInsertlacUV5 driving Streptococcus pyogenes Cas9 VRKG(D1135V/S1136R/D1332K/R1333G) and hEGFP-sgRNA
UseCRISPR and Synthetic BiologyExpressionBacterialMutationD1135V, S1136R, D1332K and R1333G mutations in Sp…PromoterlacUV5 driving Cas9 VRKG and UV5 driving sgRNAAvailable SinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET51b-Strep-SsOGT-H5-His
Plasmid#167277PurposeOverexpression of a sulfolobal equivalent of SNAP-tag in E. coliDepositorInsertSsOGT-H5
TagsHis x10 and Strep-tagExpressionBacterialPromoterT7Available SinceApril 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET11-PC-H6
Plasmid#183777PurposeExpress PC-H6 in E. coli. Purified PC-H6 was used for for generating antibodies in rabbits and for GST-CBP pull-down assays.DepositorAvailable SinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-DEST42-SARA-V5-His6
Plasmid#67431PurposeExpression of hSara with C-term V5 and His6 tag in E. coliDepositorAvailable SinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFS_1112_pET30_pCat-tetR-Term-ptetA-FsRT-Cas1(opt)-Cas2(opt)-3xTerm_J23109-Leader-ARRAY2-DR2-FaqI_Term_NotI
Plasmid#184740Purposeencodes aTc inducible FsRT-Cas1–Cas2 expression cassette and FsCRISPR Array 2 transcribed by BBa_J23109DepositorInsertFsRT-Cas1(opt)-Cas2(opt)
ExpressionBacterialMutationsequence codon optimized for expression in E. coliPromoterpTetAAvailable SinceMay 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTAMAHISTEV_Ab-PiuA
Plasmid#128957Purposeexpresses Acinetobacter baumannii PiuA in E. coliDepositorInsertTonB-dependent siderophore receptor
TagsTEV protease cleavable 7xHis and TamAExpressionBacterialMutationSignal peptide sequence has been removedPromoterT7Available SinceAug. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAGHa-SERBP1(189-400)
Plasmid#172315PurposeExpression of the C-terminal (189-400) domain of SERBP1 in E. coli.DepositorInsertPlasminogen Activator Inhibitor 1 RNA-binding protein, residues 189-400 (SERBP1 Human)
TagsHis8ExpressionBacterialMutationdeleted 1-188, 401-408Available SinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28-p53 (1-73) (72R)
Plasmid#62082PurposeExpression of human p53 (1-73) (72R) in e. coliDepositorInsertp53 (TP53 Human)
TagsHIsExpressionBacterialMutationcontains TAD residues 1-73; 72RPromoterT7Available SinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET30a-ASXL1-HARE-HTH domain
Plasmid#107164PurposeExpresses murine ASXL1 HARE-HTH domain (amino acids 2-83) in E. coliDepositorInsertASXL1 (Asxl1 Mouse)
TagsFlagExpressionBacterialMutationContains amino acids 2-83PromoterT7Available SinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pQTEV-CUTC
Plasmid#34801DepositorInsertcutC copper transporter homolog (E. coli) (CUTC Human)
TagsHis and StrepExpressionBacterialAvailable SinceApril 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET22b-ecDHFR-N23PP/G51PEKN
Plasmid#118583PurposeBacterial expression of E.coli DHFR N23PP and G51PEKN mutantDepositorInsertDHFR
ExpressionBacterialMutationN23 mutated to P23, G51 mutated to P51, a Pro res…PromoterT7Available SinceDec. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTrc99S-ssDsbA-PorA-4xDQNAT
Plasmid#128400PurposePeriplasmic expression of N. meningitidis PorA with C terminal 4xDQNAT glycosylation sites in E. coliDepositorInsertN. meningitidis PorA
Tags4xDQNAT glycosylation tag, 6xHis tag, and DsbA si…ExpressionBacterialPromotertrcAvailable SinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET51b-His-TEV-SNAP-tag_cx
Plasmid#167273PurposeOverexpression of crystallizing SNAP-tag variant (P179R) in E. coliDepositorInsertSNAP-tag
TagsHis x10ExpressionBacterialMutationP179RPromoterT7Available SinceApril 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKD265
Plasmid#125095PurposeExpression of torR(1-134aa)-psdR(134-237aa), ompR137 chimera under PLtetO-1, output Promoter PpsdA110DepositorInsertstorR(REC)-psdR(DBD)137
sfgfp
tetR
UseSynthetic BiologyExpressionBacterialAvailable SinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSR322
Plasmid#125116PurposeExpression of uhpA(1-123aa)-ydfI(126-213aa), narL131 chimera under PLtetO-1, output Promoter PydfJ115DepositorInsertsuhpA(REC)-ydfI(DBD)131
sfgfp
tetR
UseSynthetic BiologyExpressionBacterialAvailable SinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSR40.15
Plasmid#125082PurposeExpression of ompR(1-137aa)-ccaR(127-234aa) chimera under PLtetO-1, output Promoter PcpcG2-172DepositorInsertsompR(REC)-ccaR(DBD)137
sfgfp
tetR
UseSynthetic BiologyExpressionBacterialAvailable SinceMay 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSR40.29
Plasmid#125088PurposeExpression of ompR(1-139aa)-ccaR(129-234aa) chimera under PLtetO-1, output Promoter PcpcG2-172DepositorInsertsompR(REC)-ccaR(DBD)139
sfgfp
tetR
UseSynthetic BiologyExpressionBacterialAvailable SinceMay 23, 2019AvailabilityAcademic Institutions and Nonprofits only