We narrowed to 2,347 results for: puc19

1. pMT1474

   Plasmid

   #228321

   Purpose

   2,4-Diacetylphloroglucinol (DAPG)-regulatable EGFP expression

   Depositor

   Jun Ishii

   Article

   Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi

   Insert

   EGFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   2,4-Diacetylphloroglucinol-regulatable synthetic …

   Available Since

   March 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pMT1475

   Plasmid

   #228322

   Purpose

   2,4-Diacetylphloroglucinol (DAPG)-regulatable EGFP expression

   Depositor

   Jun Ishii

   Article

   Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi

   Insert

   EGFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   2,4-Diacetylphloroglucinol-regulatable synthetic …

   Available Since

   March 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMT1481

   Plasmid

   #228323

   Purpose

   2,4-Diacetylphloroglucinol (DAPG)-regulatable EGFP expression

   Depositor

   Jun Ishii

   Article

   Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi

   Insert

   EGFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   2,4-Diacetylphloroglucinol-regulatable synthetic …

   Available Since

   March 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pMT1482

   Plasmid

   #228324

   Purpose

   2,4-Diacetylphloroglucinol (DAPG)-regulatable EGFP expression

   Depositor

   Jun Ishii

   Article

   Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi

   Insert

   EGFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   2,4-Diacetylphloroglucinol-regulatable synthetic …

   Available Since

   March 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pMT1483

   Plasmid

   #228325

   Purpose

   2,4-Diacetylphloroglucinol (DAPG)-regulatable EGFP expression

   Depositor

   Jun Ishii

   Article

   Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi

   Insert

   EGFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   2,4-Diacetylphloroglucinol-regulatable synthetic …

   Available Since

   March 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pMT1328

   Plasmid

   #228302

   Purpose

   2,4-Diacetylphloroglucinol (DAPG)-regulatable EGFP expression

   Depositor

   Jun Ishii

   Article

   Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi

   Insert

   EGFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   2,4-Diacetylphloroglucinol-regulatable synthetic …

   Available Since

   March 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pMT1341

   Plasmid

   #228303

   Purpose

   2,4-Diacetylphloroglucinol (DAPG)-regulatable EGFP expression

   Depositor

   Jun Ishii

   Article

   Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi

   Insert

   EGFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   2,4-Diacetylphloroglucinol-regulatable synthetic …

   Available Since

   March 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pMT1342

   Plasmid

   #228304

   Purpose

   2,4-Diacetylphloroglucinol (DAPG)-regulatable EGFP expression

   Depositor

   Jun Ishii

   Article

   Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi

   Insert

   EGFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   2,4-Diacetylphloroglucinol-regulatable synthetic …

   Available Since

   March 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMT1343

   Plasmid

   #228305

   Purpose

   2,4-Diacetylphloroglucinol (DAPG)-regulatable EGFP expression

   Depositor

   Jun Ishii

   Article

   Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi

   Insert

   EGFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   2,4-Diacetylphloroglucinol-regulatable synthetic …

   Available Since

   March 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMT1344

    Plasmid

    #228306

    Purpose

    2,4-Diacetylphloroglucinol (DAPG)-regulatable EGFP expression

    Depositor

    Jun Ishii

    Article

    Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi

    Insert

    EGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    2,4-Diacetylphloroglucinol-regulatable synthetic …

    Available Since

    March 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMT1345

    Plasmid

    #228307

    Purpose

    2,4-Diacetylphloroglucinol (DAPG)-regulatable EGFP expression

    Depositor

    Jun Ishii

    Article

    Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi

    Insert

    EGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    2,4-Diacetylphloroglucinol-regulatable synthetic …

    Available Since

    March 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMT1346

    Plasmid

    #228308

    Purpose

    2,4-Diacetylphloroglucinol (DAPG)-regulatable EGFP expression

    Depositor

    Jun Ishii

    Article

    Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi

    Insert

    EGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    2,4-Diacetylphloroglucinol-regulatable synthetic …

    Available Since

    March 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pMT1347

    Plasmid

    #228309

    Purpose

    2,4-Diacetylphloroglucinol (DAPG)-regulatable EGFP expression

    Depositor

    Jun Ishii

    Article

    Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi

    Insert

    EGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    2,4-Diacetylphloroglucinol-regulatable synthetic …

    Available Since

    March 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMT1348

    Plasmid

    #228310

    Purpose

    2,4-Diacetylphloroglucinol (DAPG)-regulatable EGFP expression

    Depositor

    Jun Ishii

    Article

    Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi

    Insert

    EGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    2,4-Diacetylphloroglucinol-regulatable synthetic …

    Available Since

    March 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMT1349

    Plasmid

    #228311

    Purpose

    2,4-Diacetylphloroglucinol (DAPG)-regulatable EGFP expression

    Depositor

    Jun Ishii

    Article

    Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi

    Insert

    EGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    2,4-Diacetylphloroglucinol-regulatable synthetic …

    Available Since

    March 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMT1350

    Plasmid

    #228312

    Purpose

    2,4-Diacetylphloroglucinol (DAPG)-regulatable EGFP expression

    Depositor

    Jun Ishii

    Article

    Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi

    Insert

    EGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    2,4-Diacetylphloroglucinol-regulatable synthetic …

    Available Since

    March 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMT1351

    Plasmid

    #228313

    Purpose

    2,4-Diacetylphloroglucinol (DAPG)-regulatable EGFP expression

    Depositor

    Jun Ishii

    Article

    Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi

    Insert

    EGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    2,4-Diacetylphloroglucinol-regulatable synthetic …

    Available Since

    March 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMT949

    Plasmid

    #228289

    Purpose

    2,4-Diacetylphloroglucinol (DAPG)-regulatable EGFP expression

    Depositor

    Jun Ishii

    Article

    Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi

    Insert

    EGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    2,4-Diacetylphloroglucinol-regulatable synthetic …

    Available Since

    March 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMT951

    Plasmid

    #228291

    Purpose

    2,4-Diacetylphloroglucinol (DAPG)-regulatable EGFP expression

    Depositor

    Jun Ishii

    Article

    Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi

    Insert

    EGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    2,4-Diacetylphloroglucinol-regulatable synthetic …

    Available Since

    March 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMT952

    Plasmid

    #228292

    Purpose

    2,4-Diacetylphloroglucinol (DAPG)-regulatable EGFP expression

    Depositor

    Jun Ishii

    Article

    Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi

    Insert

    EGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    2,4-Diacetylphloroglucinol-regulatable synthetic …

    Available Since

    March 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pMT958

    Plasmid

    #228294

    Purpose

    2,4-Diacetylphloroglucinol (DAPG)-regulatable EGFP expression

    Depositor

    Jun Ishii

    Article

    Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi

    Insert

    EGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    2,4-Diacetylphloroglucinol-regulatable synthetic …

    Available Since

    March 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pMT959

    Plasmid

    #228295

    Purpose

    2,4-Diacetylphloroglucinol (DAPG)-regulatable EGFP expression

    Depositor

    Jun Ishii

    Article

    Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi

    Insert

    EGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    2,4-Diacetylphloroglucinol-regulatable synthetic …

    Available Since

    March 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pMT960

    Plasmid

    #228296

    Purpose

    2,4-Diacetylphloroglucinol (DAPG)-regulatable EGFP expression

    Depositor

    Jun Ishii

    Article

    Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi

    Insert

    EGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    2,4-Diacetylphloroglucinol-regulatable synthetic …

    Available Since

    March 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pMT961

    Plasmid

    #228297

    Purpose

    2,4-Diacetylphloroglucinol (DAPG)-regulatable EGFP expression

    Depositor

    Jun Ishii

    Article

    Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi

    Insert

    EGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    2,4-Diacetylphloroglucinol-regulatable synthetic …

    Available Since

    March 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pMT1014

    Plasmid

    #228298

    Purpose

    Homoserine lactone (HSL)-regulatable EGFP expression

    Depositor

    Jun Ishii

    Article

    Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi

    Insert

    EGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Homoserine lactone (HSL)-regulatable synthetic pr…

    Available Since

    March 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pKN108

    Plasmid

    #228284

    Purpose

    2,4-Diacetylphloroglucinol (DAPG)-regulatable EGFP expression

    Depositor

    Jun Ishii

    Article

    Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi

    Insert

    EGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    2,4-Diacetylphloroglucinol-regulatable synthetic …

    Available Since

    March 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pMT856

    Plasmid

    #228287

    Purpose

    2,4-Diacetylphloroglucinol (DAPG)-regulatable EGFP expression

    Depositor

    Jun Ishii

    Article

    Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi

    Insert

    EGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    2,4-Diacetylphloroglucinol-regulatable synthetic …

    Available Since

    March 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pIN1 (gpdA-cTerm-mGL-ptrA)

    Plasmid

    #188735

    Purpose

    Expresses mGreenlantern for C-terminal tagging

    Depositor

    Michael Bromley

    Article

    Storer et al Antimicrob Agents Chemother. 2024 Nov 6;

    Insert

    gdpA-mGreenlantern PtrA
    

    Use

    Filamentous fungal expression

    Tags

    mGreenlantern

    Expression

    Mutation

    Promoter

    gpdA

    Available Since

    Nov. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pIN2 (gpdA-nTerm-mGL-ptrA)

    Plasmid

    #188736

    Purpose

    Expresses mGreenlantern for N-terminal tagging

    Depositor

    Michael Bromley

    Article

    Storer et al Antimicrob Agents Chemother. 2024 Nov 6;

    Insert

    gdpA-mGreenlantern PtrA
    

    Use

    Filamentous fungal expression

    Tags

    mGreenlantern

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pIN3 (gpdA-cTerm-Citrine2-ptrA)

    Plasmid

    #188737

    Purpose

    Expresses Citrine2 for C-terminal tagging

    Depositor

    Michael Bromley

    Article

    Storer et al Antimicrob Agents Chemother. 2024 Nov 6;

    Insert

    gdpA-Citrine2 PtrA
    

    Use

    Filamentous fungal expression

    Tags

    Citrine2

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pIN4 (gpdA-nTerm-Citrine2-ptrA)

    Plasmid

    #188738

    Purpose

    Expresses Citrine2 for N-terminal tagging

    Depositor

    Michael Bromley

    Article

    Storer et al Antimicrob Agents Chemother. 2024 Nov 6;

    Insert

    gdpA-Citrine2 PtrA
    

    Use

    Filamentous fungal expression

    Tags

    Citrine2

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pIN5 (gpdA-cTerm-mTurqoise2-ptrA)

    Plasmid

    #188739

    Purpose

    Expresses mTurqoise2 for C-terminal tagging

    Depositor

    Michael Bromley

    Article

    Storer et al Antimicrob Agents Chemother. 2024 Nov 6;

    Insert

    gdpA-mTurquoise PtrA
    

    Use

    Filamentous fungal expression

    Tags

    mTurquoise

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pIN6 (gpdA-nTerm-mTurqoise2-ptrA)

    Plasmid

    #188740

    Purpose

    Expresses mTurqoise2 for N-terminal tagging

    Depositor

    Michael Bromley

    Article

    Storer et al Antimicrob Agents Chemother. 2024 Nov 6;

    Insert

    gdpA-mTurqoise2 PtrA
    

    Use

    Filamentous fungal expression

    Tags

    mGreenlantern

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pGGF-bGH polyA-G

    Plasmid

    #218902

    Purpose

    golden gate cloning

    Depositor

    Sven Eyckerman

    Article

    Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

    Insert

    bGH polyA
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pGGB-V5-TurboID-MUTT2A-C

    Plasmid

    #218897

    Purpose

    golden gate cloning

    Depositor

    Sven Eyckerman

    Article

    Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

    Insert

    V5-TurboID-MUTT2A
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pGGB-V5-TurboID-T2A-C

    Plasmid

    #218896

    Purpose

    golden gate cloning

    Depositor

    Sven Eyckerman

    Article

    Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

    Insert

    V5-TurboID-T2A
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pITESceIcmd

    Plasmid

    #218288

    Purpose

    Introduction of a cyanamide-inducible I-SceI expression cassette, use in combination with pITEdv1

    Depositor

    Claudia Vickers

    Article

    McDonnell et al bioRxiv 2024.03.26.586389

    Insert

    HO(-253, -1)-tSynth3

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    May 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. ATP1A1_T804N_hPGK1_mScarlet-I-TOSI_Donor

    Plasmid

    #173208

    Purpose

    HDR donor plasmid to introduce the T804N mutation conferring cellular resistance to ouabain and the mScarlet-I mTOR signaling indicator (TOSI) cassette to ATP1A1 intron 17.

    Depositor

    Yannick Doyon

    Article

    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

    Insert

    ATP1A1-T804N mScarlet-I-TOSI donor
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hPGK1

    Available Since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pPAP_scFv

    Plasmid

    #183733

    Purpose

    Expresses anti-lysozyme scFv antibody

    Depositor

    Jun Ishii

    Article

    Ito et al Commun Biol. 2022 Jun 8;5(1):561. doi: 1

    Insert

    Anti-lysozyme scFv
    

    Use

    Tags

    His6-tag

    Expression

    Yeast

    Mutation

    Promoter

    AOX1 promoter

    Available Since

    Feb. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pCA23-sgRNA vector-U6-sgTS2 (SpCas9)

    Plasmid

    #199454

    Purpose

    U6-driven sgRNA targeting TS2 sequence (GGAGCTTACTGAGACTCTTC)

    Depositor

    Baohui Chen

    Article

    Liang et al Nat Commun. 2022 Nov 28;13(1):7329. doi:

    Insert

    TS2 sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    mouse U6

    Available Since

    May 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pCA28-pMa-PspCas13b crRNA-TS1

    Plasmid

    #199459

    Purpose

    U6-driven crRNA targeting TS1 sequence (CCTCCTCGGAGAGCATCGGTGC )

    Depositor

    Baohui Chen

    Article

    Liang et al Nat Commun. 2022 Nov 28;13(1):7329. doi:

    Insert

    TS1 crRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    mouse U6

    Available Since

    May 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pRD424

    Plasmid

    #178202

    Purpose

    Expression of Rok from the hns promoter

    Depositor

    Remus Dame

    Article

    Erkelens et al Nucleic Acids Res. 2022 Nov 21. pii: 683

    Insert

    phns + rok
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    hns promoter

    Available Since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pRD410

    Plasmid

    #178200

    Purpose

    Expression of sRok from the hns promoter

    Depositor

    Remus Dame

    Article

    Erkelens et al Nucleic Acids Res. 2022 Nov 21. pii: 683

    Insert

    phns + srok
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    hns promoter

    Available Since

    Nov. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pIALD2HMGr

    Plasmid

    #185867

    Purpose

    Knocking out ALD6; expressing acetylating aldehyde dehydrogenase (Lactobacillus reuteri pduP and L. reuteri EutE) and an NADH-preferring HMG-CoA reductase (Delftia acidovorans mvaA) in S. cerevisiae

    Depositor

    Claudia Vickers

    Article

    Hayat et al Microb Biotechnol. 2021 Nov;14(6):2627-2

    Insert

    ALD6(-125, 40)> PSk.GAL1>Da.mvaA>TPGK1-PADH1> Lr.pduP> TPDC1-PGAL2>Lr.EutE-ALD6(1054, 1749)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    None

    Promoter

    Available Since

    Sept. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pAT15541_dCBE-SuperFi-Cas9

    Plasmid

    #184371

    Purpose

    Mammalian expression of nuclease inactive SuperFi-Cas9 CBE base editor

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2022 Nov 11;13(1):6858. doi:

    Insert

    dCBE-SuperFi-Cas9
    

    Use

    CRISPR

    Tags

    3X FLAG, SV40 NLS, and UGI

    Expression

    Mammalian

    Mutation

    D10A, H840A, Y1010D, Y1013D, Y1016D, V1018D, R101…

    Promoter

    EF-1α core promoter

    Available Since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pAT15543_dABE-SuperFi-Cas9

    Plasmid

    #184373

    Purpose

    Mammalian expression of nuclease inactive SuperFi-Cas9 ABE7 base editor

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2022 Nov 11;13(1):6858. doi:

    Insert

    dABE-SuperFi-Cas9
    

    Use

    CRISPR

    Tags

    FLAG, SV40 NLS, and nucleoplasmin NLS

    Expression

    Mammalian

    Mutation

    D10A, H840A, Y1010D, Y1013D, Y1016D, V1018D, R101…

    Promoter

    EF-1α core promoter

    Available Since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pAT15545_dABE8e-SuperFi-Cas9

    Plasmid

    #184375

    Purpose

    Mammalian expression of nuclease inactive SuperFi-Cas9 ABE8e base editor

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2022 Nov 11;13(1):6858. doi:

    Insert

    dABE8e-SuperFi-Cas9
    

    Use

    CRISPR

    Tags

    Nucleoplasmin NLS and SV40 NLS

    Expression

    Mammalian

    Mutation

    D10A, H840A, Y1010D, Y1013D, Y1016D, V1018D, R101…

    Promoter

    EF-1α core promoter

    Available Since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. ATP1A1_G3_EBFP_Nick_Dual_sgRNA

    Plasmid

    #178094

    Purpose

    Control vector for coselection for PE3 in human cells. Tandem expression of ATP1A1 G3 and EBFP Nick sgRNAs from two independent U6 promoters.

    Depositor

    Yannick Doyon

    Article

    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

    Insert

    ATP1A1 G3 + EBFP nick sgRNAs
    

    Use

    CRISPR; Prime editing

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tandem U6 promoters

    Available Since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. ATP1A1_G3_EGFP_Nick_Dual_sgRNA

    Plasmid

    #178095

    Purpose

    Control vector for coselection for PE3b in human cells. Tandem expression of ATP1A1 G3 and EGFP Nick sgRNAs from two independent U6 promoters.

    Depositor

    Yannick Doyon

    Article

    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

    Insert

    ATP1A1 G3 + EGFP nick sgRNAs
    

    Use

    CRISPR; Prime editing

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tandem U6 promoters

    Available Since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. ATP1A1_G3_MTOR_Nick_Exon-44_Dual_sgRNA

    Plasmid

    #178096

    Purpose

    Control vector for coselection for PE3 in human cells. Tandem expression of ATP1A1 G3 and MTOR Nick exon-44 sgRNAs from two independent U6 promoters.

    Depositor

    Yannick Doyon

    Article

    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

    Insert

    ATP1A1 G3 + MTOR nick exon-44 sgRNAs
    

    Use

    CRISPR; Prime editing

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tandem U6 promoters

    Available Since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge