We narrowed to 1,500 results for: RCA

1. pCDH_Str-KDEL_ManII-SBP-mCherry

   Plasmid

   #65259

   Purpose

   synchronize trafficking of ManII from the ER (RUSH system)

   Depositor

   Franck Perez

   Article

   Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

   Insert

   Streptavidin-KDEL and truncated Mannosidase II
   

   Use

   Lentiviral

   Tags

   Streptavidin Binding Protein (SBP) and mCherry

   Expression

   Mutation

   contains only amino acids 1 to 116 of ManII

   Promoter

   CMV

   Available Since

   Aug. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. AAV-P(Per2)-DIO-intron2-dLUC

   Plasmid

   #110059

   Purpose

   Per2 transcription luciferase reporter

   Depositor

   Eric Erquan Zhang

   Article

   Mei et al Proc Natl Acad Sci U S A. 2018 Apr 2. pi

   Insert

   Per2 promoter and luciferase (Per2 Mouse)
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Per2

   Available Since

   May 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. Str-KDEL_ManII-SBP-EGFP

   Plasmid

   #65252

   Purpose

   synchronize trafficking of ManII from the ER (RUSH system)

   Depositor

   Franck Perez

   Article

   Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

   Insert

   Streptavidin-KDEL and truncated Mannosidase II
   

   Use

   Tags

   EGFP and Streptavidin Binding Protein (SBP)

   Expression

   Mammalian

   Mutation

   contains only amino acids 1 to 116 of ManII

   Promoter

   CMV

   Available Since

   Aug. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGL3

   Plasmid

   #48743

   Purpose

   Expression of luciferase driven by truncated mPer2 promoter region (bases -1128 to +1060 with respect to transcription start site at +1)

   Depositor

   Joseph Takahashi

   Article

   Yoo et al Proc Natl Acad Sci U S A. 2005 Feb 15;10

   Insert

   truncated mPer2 promoter/enhancer region (-1128 to +1060)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   truncated promoter region (see pGL6)

   Promoter

   Available Since

   Dec. 19, 2013

   Availability

   Academic Institutions and Nonprofits only

5. AICSDP-41: ATP2A2-mEGFP

   Plasmid

   #114405

   Purpose

   Homology arms and mEGFP-linker sequence for N-terminus tagging of human ATP2A2

   Depositor

   Allen Institute for Cell Science

   Insert

   ATP2A2 Homology Arms with mEGFP-linker (ATP2A2 Human)
   

   Use

   CRISPR; Donor template

   Tags

   mEGFP-linker

   Expression

   Mammalian

   Mutation

   homology arms contain point mutations to disrupt …

   Promoter

   Available Since

   Sept. 4, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pBS-ACTB-C200-GFPfr1

   Plasmid

   #169798

   Purpose

   THe donor vector for the ACTB gene locus.

   Depositor

   Natsuko Chiba

   Article

   Yoshino et al Sci Rep. 2019 Feb 7;9(1):1644. doi: 10.1

   Insert

   Donor sequence to ACTB gene for HR
   

   Use

   Hr donor vector for human actb gene.

   Tags

   Expression

   Mutation

   Partial sequence of GFP is inserted before the st…

   Promoter

   Available Since

   Nov. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. MSCV PIG Pu.1

   Plasmid

   #66982

   Purpose

   Retroviral expression plasmid for mouse Pu.1 expression; containing bicistronic IRES-GFP

   Depositor

   Christopher Vakoc

   Article

   Roe et al Mol Cell. 2015 May 12. pii: S1097-2765(1

   Insert

   Spi1 (Spi1 Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   LTR

   Available Since

   July 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSMP-RING1_2

   Plasmid

   #36356

   Depositor

   George Daley

   Article

   Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

   Insert

   RING1 (RING1 Human)
   

   Use

   RNAi and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 2, 2012

   Availability

   Academic Institutions and Nonprofits only

9. pGL3 Pro E2

   Plasmid

   #48749

   Purpose

   Expression of luciferase driven by mPer2 promoter fragment containing E-box2 (bases -112 to +98 with respect to transcription start site at +1) and SV40 promoter (backbone)

   Depositor

   Joseph Takahashi

   Article

   Yoo et al Proc Natl Acad Sci U S A. 2005 Feb 15;10

   Insert

   mPer2 promoter/enhancer region containing E-box2 (-112 to +98)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBS-KLF7-200-GFPfr1

    Plasmid

    #169799

    Purpose

    THe donor vector for the KLF7 gene locus.

    Depositor

    Natsuko Chiba

    Article

    Yoshino et al Sci Rep. 2019 Feb 7;9(1):1644. doi: 10.1

    Insert

    Donor sequence to KLF7 neighbouring region for HR
    

    Use

    Hr donor vector for human actb gene.

    Tags

    Expression

    Mutation

    Partial sequence of GFP is inserted the sequence …

    Promoter

    Available Since

    Aug. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. LentiCRISPRv2-KLF7-1

    Plasmid

    #169797

    Purpose

    Expresses Cas9 and guide RNA for the site neighbouring KLF7 gene.

    Depositor

    Natsuko Chiba

    Article

    Yoshino et al Sci Rep. 2019 Feb 7;9(1):1644. doi: 10.1

    Insert

    guide RNA for KLF7 neighbouring region
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    June 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. ST-SBP-mCherry_puromycin

    Plasmid

    #65271

    Purpose

    ST reporter only ( no hook) (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    truncated sialyltransferase (ST6GAL1 Human)
    

    Use

    Tags

    Streptavidin Binding Protein (SBP) and mCherry

    Expression

    Mammalian

    Mutation

    contains only amino acids 1 to 43 of ST

    Promoter

    CMV

    Available Since

    June 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAGGS-FLAG-CaMKIID-K43M

    Plasmid

    #186830

    Purpose

    Expresses FLAG-CaMKIID-K43M in mammalian cells

    Depositor

    Hung-Chun Chang

    Article

    Li et al Commun Biol. 2022 Jul 28;5(1):750. doi:

    Insert

    Camk2d (Camk2d Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changed Lysine 43 to Methionine

    Promoter

    Available Since

    April 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. Ii-Str_ST-SBP-mCherry

    Plasmid

    #65269

    Purpose

    synchronize trafficking of ST from the ER (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    Ii-Streptavidin and truncated sialyltransferase (ST6GAL1 Human)
    

    Use

    Tags

    Streptavidin Binding Protein (SBP) and mCherry

    Expression

    Mammalian

    Mutation

    contains only amino acids 1 to 43 of ST

    Promoter

    CMV

    Available Since

    June 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. HA-VHL W117R-pBabe-puro

    Plasmid

    #19239

    Depositor

    William Kaelin

    Article

    Li et al Mol Cell Biol. 2007 Aug . 27(15):5381-92

    Insert

    von Hippel-Lindau tumor suppressor (VHL) (VHL Human)
    

    Use

    Retroviral

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    changed Tryptophan 117 to Arginin

    Promoter

    Available Since

    Oct. 1, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSMP-G9A_1

    Plasmid

    #36395

    Depositor

    George Daley

    Article

    Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

    Insert

    G9A (EHMT2 Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 2, 2012

    Availability

    Academic Institutions and Nonprofits only

17. pG4-CMV-luc

    Plasmid

    #46322

    Depositor

    Steven Brown

    Article

    Kowalska et al Mol Cell Biol. 2012 Nov;32(22):4585-94.

    Insert

    5xGal4
    

    Use

    Luciferase

    Tags

    CMV promoter and luciferase

    Expression

    Mammalian

    Mutation

    five copies of a multimerized GAL4 (5×GAL4) site …

    Promoter

    CMV

    Available Since

    July 5, 2013

    Availability

    Academic Institutions and Nonprofits only

18. pAM5080

    Plasmid

    #85106

    Purpose

    Ptrc-yfp-kaiC expressed from NSII-Km vector constructed by seamless assembly. LacI expressed in opposite direction as Ptrc promoter. Functional fusion expres

    Depositor

    Susan Golden

    Article

    Cohen et al Curr Biol. 2014 Aug 18;24(16):1836-44. d

    Insert

    YFP-KaiC
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. Str-KDEL_ST-SBP-tagBFP

    Plasmid

    #65266

    Purpose

    synchronize trafficking of ST from the ER (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    Str-KDEL and truncated sialyltransferase (ST6GAL1 Human)
    

    Use

    Tags

    Streptavidin Binding Protein (SBP) and tagBFP

    Expression

    Mammalian

    Mutation

    contains only amino acids 1 to 43 of ST

    Promoter

    CMV

    Available Since

    June 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSMP-G9A_4

    Plasmid

    #36334

    Depositor

    George Daley

    Article

    Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

    Insert

    G9A (EHMT2 Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 2, 2012

    Availability

    Academic Institutions and Nonprofits only