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IA201: pMAGIC (R4-R3) NLS-Sa dCas9-NLS

Plasmid

#121821

Purpose

pMAGIC R4-R3 entry plasmid, contains 2x NLS Sa-dCas9 (nuclease-dead) fusion for 3 or 4-component MultiSite Gateway Pro assembly.

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

Sa-dCas9 (open)

Use

Gateway Cloning and Synthetic Biology

Tags

NLS

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV.Syn.NES-jRGECO1b.WPRE.SV40

Plasmid

#100857

Purpose

AAV expression of jRGECO1b, a red fluorescent calcium sensor protein, from the Synapsin promoter

Depositor

Douglas Kim, GENIE Project

Article

Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

Has Service

AAV1

Insert

jRGECO1b

Use

AAV

Expression

Mammalian

Promoter

Synapsin

Available Since

Nov. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV_BiPVe3_GCaMP6f

Plasmid

#213943

Purpose

AAV construct expressing GCaMP6f driven by PV+ basket cell-targeting enhancer. Alias: pAAV_WDP0003_GCaMP6f

Depositor

Gordon Fishell

Article

Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1

Has Service

AAV PHP.eB and AAV1

Insert

GCaMP6f

Use

AAV

Available Since

July 23, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-hSynapsin1-RdLight1

Plasmid

#125708

Purpose

Expresses the red genetically encoded dopamine sensor RdLight1 in mammalian neurons.

Depositor

Lin Tian

Article

Patriarchi et al Nat Methods. 2020 Nov;17(11):1147-1155.

Has Service

AAV9

Insert

RdLight1

Use

AAV

Tags

Flag tag

Promoter

hSynapsin1

Available Since

Aug. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pMT-FLAG-DmAgo1

Plasmid

#229236

Purpose

Expresses FLAG-tagged Ago1 in Drosophila S2 cells

Depositor

Karina Jouravleva

Article

Vega-Badillo et al Nat Commun. 2026 Jan 20. doi: 10.1038/s4

Insert

Argonaute1 (AGO1 Fly)

Tags

FLAG

Expression

Insect

Promoter

MT promoter

Available Since

Feb. 2, 2026

Availability

Academic Institutions and Nonprofits only

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pGEX-Magi-3-PDZ-5

Plasmid

#250107

Purpose

Bacterial expression of Magi-3 PDZ domain 5

Depositor

Mark Bedford

Article

Espejo et al J Biol Chem. 2017 Feb 10;292(6):2255-226

Insert

Magi-3 (5/6) (Magi3 Mouse)

Expression

Bacterial

Mutation

None

Available Since

Jan. 27, 2026

Availability

Academic Institutions and Nonprofits only

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pGEX-Magi-2-PDZ-6

Plasmid

#250105

Purpose

Bacterial expression of Magi-2 PDZ domain 6

Depositor

Mark Bedford

Article

Espejo et al J Biol Chem. 2017 Feb 10;292(6):2255-226

Insert

Magi-2 (6/6) (Magi2 Mouse)

Expression

Bacterial

Mutation

None

Available Since

Jan. 27, 2026

Availability

Academic Institutions and Nonprofits only

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pGEX-Magi-3-PDZ-1

Plasmid

#250106

Purpose

Bacterial expression of Magi-3 PDZ domain 1

Depositor

Mark Bedford

Article

Espejo et al J Biol Chem. 2017 Feb 10;292(6):2255-226

Insert

Magi-3 (1/6) (Magi3 Mouse)

Expression

Bacterial

Mutation

None

Available Since

Jan. 26, 2026

Availability

Academic Institutions and Nonprofits only

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pGEX-Magi-1-PDZ-4

Plasmid

#250101

Purpose

Bacterial expression of Magi-1 PDZ domain 4

Depositor

Mark Bedford

Article

Espejo et al J Biol Chem. 2017 Feb 10;292(6):2255-226

Insert

Magi-1 (4/6) (Magi1 Mouse)

Expression

Bacterial

Mutation

None

Available Since

Jan. 26, 2026

Availability

Academic Institutions and Nonprofits only

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pGEX-Magi-1-PDZ-6

Plasmid

#250102

Purpose

Bacterial expression of Magi-1 PDZ domain 6

Depositor

Mark Bedford

Article

Espejo et al J Biol Chem. 2017 Feb 10;292(6):2255-226

Insert

Magi-1 (6/6) (Magi1 Mouse)

Expression

Bacterial

Mutation

None

Available Since

Jan. 26, 2026

Availability

Academic Institutions and Nonprofits only

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pGEX-Magi-2-PDZ-5

Plasmid

#250104

Purpose

Bacterial expression of Magi-2 PDZ domain 5

Depositor

Mark Bedford

Article

Espejo et al J Biol Chem. 2017 Feb 10;292(6):2255-226

Insert

Magi-2 (5/6) (Magi2 Mouse)

Expression

Bacterial

Mutation

None

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pGEX-Magi-2-PDZ-2

Plasmid

#250103

Purpose

Bacterial expression of Magi-2 PDZ domain 2

Depositor

Mark Bedford

Article

Espejo et al J Biol Chem. 2017 Feb 10;292(6):2255-226

Insert

Magi-2 (2/6) (Magi2 Mouse)

Expression

Bacterial

Mutation

None

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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psBIG1a ActBT2ATetP2AP - mAGH

Plasmid

#229973

Purpose

Harbor sgRNA+PAM sequence of ActinB gene for knockin using CRISPR and Homology Independent Targeted Integration. Contains last ActB exon fused T2A-tet regulatory elemeny-P2A-puro and mAGNLS-T2A-Hygro

Depositor

Martin Pelosse

Article

Pelosse et al Synthetic Biology 2025.02.13

Insert

ActBT2ATetP2AP - mAGH

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pNLF1-C_MAGEA3:1-314

Plasmid

#210977

Purpose

Mammalian expression of full-length human MAGEA3. C-terminal NanoLuc luciferase tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

MAGEA3:M1-E314 (MAGEA3 Human)

Tags

NanoLuc luciferase

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmAGO3_O

Plasmid

#147070

Purpose

Insect Expression of DmAGO3

Depositor

Elisa Izaurralde

Insert

DmAGO3 (AGO3 Fly)

Expression

Insect

Mutation

2 silent an two non silent S105P, S199L mutations…

Available Since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

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promoter-less mRFP1_Magenta

Plasmid

#183140

Purpose

BioBrick pSB1C3 plasmid encoding a promoter-less version of mRFP1_Magenta

Depositor

Anthony Forster

Article

Bao et al Anal Biochem. 2020 Sep 3:113936. doi: 10

Insert

RBS, mRFP1E_Magenta

Use

Synthetic Biology

Mutation

BioBrick sites removed

Available Since

Sept. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pHH0103 MAGI1 WW domain #1

Plasmid

#104242

Purpose

Bacterial expression of WW domain #1 from MAGI1

Depositor

Sachdev Sidhu, Marius Sudol

Insert

MAGI1 WW domain #1 (MAGI1 Human)

Tags

GST

Expression

Bacterial

Mutation

codon optimized for expression in bacteria

Promoter

tac

Available Since

March 2, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103 MAGI1 WW domain #2

Plasmid

#104243

Purpose

Bacterial expression of WW domain #2 from MAGI1

Depositor

Sachdev Sidhu, Marius Sudol

Insert

MAGI1 WW domain #2 (MAGI1 Human)

Tags

GST

Expression

Bacterial

Mutation

codon optimized for expression in bacteria

Promoter

tac

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pSG5-MAGE-TA360

Plasmid

#89141

Purpose

mammalian expression of full-length human MAGE-A11 amino acid residues 2-429 with TA360 phosphorylation site mutated

Depositor

Elizabeth Wilson

Article

Bai et al Mol Cell Biol. 2008 Mar;28(6):1947-63. d

Insert

MAGE-A11 (MAGEA11 Human)

Expression

Mammalian

Mutation

T360A

Promoter

SV40

Available Since

April 10, 2017

Availability

Academic Institutions and Nonprofits only
GAL-MAGE-2-429

Plasmid

#89126

Purpose

mammalian expression of full-length human MAGE-A11 with N-terminal GAL4 DNA binding domain

Depositor

Elizabeth Wilson

Article

Bai et al Mol Cell Biol. 2005 Feb;25(4):1238-57.

Insert

MAGE-A11 (MAGEA11 Human)

Tags

GAL4 DBD

Expression

Mammalian

Available Since

April 10, 2017

Availability

Academic Institutions and Nonprofits only