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LT1_71_TU5of6_X2homology

Plasmid

#194413

Purpose

TU5 backbone (for TU #5 for plasmid with 6 TUs) with X2 homology arms for Saccharomyces cerevisiae

Depositor

Lisbeth Olsson

Article

Torello Pianale et al ACS Synth Biol. 2023 Aug 8. doi: 10.1021

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Mutation

NA

Available Since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

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pXalb_IF1_Cascade

Plasmid

#178768

Purpose

Expression of X. albilineansCascade type I-F1 Cascade genes

Depositor

Chase Beisel

Article

Wimmer et al Mol Cell. 2022 Feb 16. pii: S1097-2765(2

Insert

X. albilineansCascade type I-F1 Cascade genes

Expression

Bacterial

Available Since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

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pXalb_IF1_Cas2-3

Plasmid

#178769

Purpose

Expression of X. albilineans type I-F1 cas2-3

Depositor

Chase Beisel

Article

Wimmer et al Mol Cell. 2022 Feb 16. pii: S1097-2765(2

Insert

X. albilineans type I-F1 cas2-3

Expression

Bacterial

Available Since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

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pEDJ508

Plasmid

#177275

Purpose

ADH1t:T7p:cgRNA_HIS3_stop:tZ

Depositor

Michael Jensen

Article

Jensen et al Nucleic Acids Res. 2021 Sep 7;49(15):e88

Insert

ADH1t:T7p:cgRNA_HIS3_stop:tZ

Expression

Bacterial and Yeast

Promoter

N/A

Available Since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pMV18

Plasmid

#128895

Purpose

Position 6 module- KanMX

Depositor

Gilles Charvin

Article

Goulev et al PLoS One. 2019 Aug 28;14(8):e0220694. do

Insert

KanMX

Use

Synthetic Biology

Expression

Yeast

Available Since

Aug. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pMV4

Plasmid

#128883

Purpose

Position 3 module- mCherry

Depositor

Gilles Charvin

Article

Goulev et al PLoS One. 2019 Aug 28;14(8):e0220694. do

Insert

mCherry

Use

Synthetic Biology

Expression

Yeast

Available Since

Aug. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pMV8

Plasmid

#128863

Purpose

Position 1 module- Linker

Depositor

Gilles Charvin

Article

Goulev et al PLoS One. 2019 Aug 28;14(8):e0220694. do

Insert

Flag as Linker

Use

Synthetic Biology

Expression

Yeast

Available Since

Aug. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pTU-A-010

Plasmid

#124418

Purpose

Slot A, contains mKO2-MarA (step 1)

Depositor

Yolanda Schaerli

Article

Santos-Moreno et al ACS Synth Biol. 2019 Jul 19;8(7):1691-16

Insert

slot A with mKO2-MarA reporter + terminator + spacer

Use

Synthetic Biology

Expression

Bacterial

Available Since

June 12, 2019

Availability

Academic Institutions and Nonprofits only

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pTU-B-010

Plasmid

#124419

Purpose

Slot B, contains sfGFP-MarAn20 (step 1)

Depositor

Yolanda Schaerli

Article

Santos-Moreno et al ACS Synth Biol. 2019 Jul 19;8(7):1691-16

Insert

slot B with sfGFP-MarAn20 reporter + terminator + spacer

Use

Synthetic Biology

Expression

Bacterial

Available Since

June 12, 2019

Availability

Academic Institutions and Nonprofits only

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pTU-C-010

Plasmid

#124420

Purpose

Slot C, contains RepA70-mKate2 (step 1)

Depositor

Yolanda Schaerli

Article

Santos-Moreno et al ACS Synth Biol. 2019 Jul 19;8(7):1691-16

Insert

slot C with RepA70-mKate2 reporter + terminator

Use

Synthetic Biology

Expression

Bacterial

Available Since

June 12, 2019

Availability

Academic Institutions and Nonprofits only

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Lasso 1

Plasmid

#69935

Purpose

The first of the two RNA lassos required for the RNA chain construction

Depositor

Alfonso Jaramillo

Article

Hassall et al Methods Mol Biol. 2015;1316:149-67. doi:

Insert

Lasso 1

Use

Synthetic Biology

Promoter

TAATACGACTCACTATAGGG

Available Since

March 11, 2016

Availability

Academic Institutions and Nonprofits only

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Lasso 2

Plasmid

#69936

Purpose

The second of the two RNA lassos required for the RNA chain construction.

Depositor

Alfonso Jaramillo

Article

Hassall et al Methods Mol Biol. 2015;1316:149-67. doi:

Insert

Lasso 2

Use

Synthetic Biology

Promoter

TAATACGACTCACTATAGGG

Available Since

March 11, 2016

Availability

Academic Institutions and Nonprofits only

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pMyo-3::oxCerulean-oxVenus::Lgg-1

Plasmid

#68044

Purpose

Lgg-1 tagged with oxCerulean-oxVenus (dFP, or double fluorescent protein), expressed in body wall muscle, for monitoring autophagy

Depositor

Dana Miller

Article

Chapin et al Aging (Albany NY). 2015 Jun 30.

Insert

pMyo-3::oxCerulean-oxVenus::Lgg-1

Expression

Worm

Available Since

Aug. 28, 2015

Availability

Academic Institutions and Nonprofits only

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mEos4b-B9D2-N-18

Plasmid

#62774

Purpose

Localization: Cilium, N1 Cloning Vector, Excitation:505 / 569, Emission:516 / 581

Depositor

Michael Davidson, Loren Looger

Insert

B9D2 (B9d2 Mouse)

Tags

mEos4a

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

March 10, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pLG-PLint

Plasmid

#33145

Depositor

Michael Rosbash

Article

Legrain et al Cell. 1989 May 19;57(4):573-83.

Insert

RP51A-synthetic minimal intron (RPS17A Budding Yeast)

Tags

LacZ

Expression

Yeast

Available Since

Dec. 8, 2011

Availability

Academic Institutions and Nonprofits only
pJBL2191

Plasmid

#71205

Purpose

pbuE STAR Antisense Expression Plasmid

Depositor

Julius Lucks

Article

Chappell et al Nat Chem Biol. 2015 Mar;11(3):214-20. do

Insert

pbuE STAR Antisense

Use

Synthetic Biology

Expression

Bacterial

Promoter

BBa_J23119

Availability

Academic Institutions and Nonprofits only

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pJBL1864

Plasmid

#71210

Purpose

Anti-anti Sense Expression Plasmid

Depositor

Julius Lucks

Article

Chappell et al Nat Chem Biol. 2015 Mar;11(3):214-20. do

Insert

Anti-anti Sense

Use

Synthetic Biology

Expression

Bacterial

Promoter

BBa_J23119

Availability

Academic Institutions and Nonprofits only

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mNeonGreen

Plasmid

#189976

Purpose

Part 3-4-5 plasmid to introduce mNeonGreen into circRNAs

Depositor

Howard Chang

Article

Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

Insert

mNeonGreen

Use

Cloning

Promoter

N/A

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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mito-dendra2

Plasmid

#55796

Purpose

photo-activatable mitochondrial marker for expression in mammalian cells

Depositor

David Chan

Article

Pham et al Genesis. 2012 Nov;50(11):833-43. doi: 10

Insert

mito-Dendra2

Expression

Mammalian

Promoter

CMV

Available Since

July 22, 2014

Availability

Academic Institutions and Nonprofits only

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ER-GCaMP6f

Plasmid

#182548

Purpose

Expresses GCaMP6f on the cytosolic side of the ER (P-450site)

Depositor

Chris Richards

Article

Aryal et al Anal Chem. 2022 Jan 21. doi: 10.1021/acs

Insert

ER localized P450

Expression

Bacterial and Mammalian

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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