We narrowed to 14,484 results for: bli

1. DDX3X - I179T in pENTR

   Plasmid

   #216576

   Purpose

   gateway cloning donor vector containing DDX3X ASD mutation

   Depositor

   Christopher Loewen, Douglas Allan

   Insert

   DDX3X (DDX3X Human)
   

   Use

   Gateway entry plasmid

   Mutation

   I179T

   Available Since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. DDX3X - R145C in PENTR

   Plasmid

   #216575

   Purpose

   gateway cloning donor vector containing DDX3X ASD mutation

   Depositor

   Christopher Loewen, Douglas Allan

   Insert

   DDX3X (DDX3X Human)
   

   Use

   Gateway entry plasmid

   Mutation

   R145C

   Available Since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. DDX3X - G123E in pENTR

   Plasmid

   #216574

   Purpose

   gateway cloning donor vector containing DDX3X ASD mutation

   Depositor

   Christopher Loewen, Douglas Allan

   Insert

   DDX3X (DDX3X Human)
   

   Use

   Gateway entry plasmid

   Mutation

   G123E

   Available Since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. DDX3X - I107V in pENTR

   Plasmid

   #216573

   Purpose

   gateway cloning donor vector containing DDX3X ASD mutation

   Depositor

   Christopher Loewen, Douglas Allan

   Insert

   DDX3X (DDX3X Human)
   

   Use

   Gateway entry plasmid

   Mutation

   I107V

   Available Since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. SOX2-3'gRNA

   Plasmid

   #215543

   Purpose

   gRNA targeting SOX2 3' terminal for CRISPR-Cas9-mediated knock-in

   Depositor

   Kai Wang

   Insert

   SOX2 (SOX2 Human)
   

   Use

   CRISPR

   Available Since

   March 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. SOX2-P2A-NLS-mClover3-IRES-Puro

   Plasmid

   #215544

   Purpose

   Donor plasmid for knock-in P2A-NLS-mClover3 into the human SOX2 locus

   Depositor

   Kai Wang

   Insert

   SOX2 homologous recombination arms with IRES-Puro selection cassette (SOX2 Human)
   

   Use

   CRISPR

   Available Since

   March 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-ApaLIE225A, K227A-HA

   Plasmid

   #211460

   Purpose

   Express mitochondrial localized ApaLI with E225A and K227A mutations

   Depositor

   Agnel Sfeir

   Article

   Fu et al Mol Cell. 2023 Oct 8:S1097-2765(23)00753

   Insert

   ApaLI-E225A-K227A
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   E225A, K227A

   Promoter

   CMV

   Available Since

   Jan. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUC-ODN-hsRPL3-CterTwStrep

   Plasmid

   #208644

   Purpose

   for long single strand DNA donnor preparation for Twin Strep tag knock-in at C ter of human RPL3. Digest with nt.BspQI and nb.BsrDI to get ssDNA. This plasmid will cause re-edit and fail knock-in.

   Depositor

   Quanfu Ma

   Insert

   Twin Strep tag with RPL3 genome HomoArm (RPL3 Human, Synthetic)
   

   Use

   CRISPR; Ssdna donnor preparation

   Tags

   Twin Strep

   Available Since

   Jan. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1-msEGFP1.8

   Plasmid

   #208619

   Purpose

   Mammalian expression of a EGFP variant. Mutations were adopted from (Addgene#135301, PMC7508230), named msEGFP1.8 because N-ter and C-ter features of that in msGFP2 (PMC7508230) not included.

   Depositor

   Quanfu Ma

   Insert

   msEGFP1.8
   

   Expression

   Mammalian

   Mutation

   (compared with pEGFP-C1, protein level) S30R, Y39…

   Promoter

   CMV

   Available Since

   Jan. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. GOSR2(G144W)-mCherry

    Plasmid

    #209887

    Purpose

    Expresses human GOSR2 (GS27) G144W-mutant fused to mChery in mammalian cells

    Depositor

    Geert van den Bogaart

    Insert

    GOSR2 (GOSR2 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Changed glycine 144 to tryptophan; this mutation …

    Promoter

    CMV

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. BASIC_SEVA_69.11

    Plasmid

    #194214

    Purpose

    BASIC DNA assembly vector

    Depositor

    Paul Freemont

    Article

    Haines et al Synth Biol (Oxf). 2022 Oct 11;7(1):ysac0

    Insert

    mScarlet counter-selection cassette
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    July 26, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pVRC8400-SARS-CoV-2-NTD-BA.4/5-AVI

    Plasmid

    #192113

    Purpose

    Expresses SARS-CoV-2 NTD protein from the BA.4 and BA.5 variants with single chain Fc, HRV3C protease cleavage site, and AVI tag

    Depositor

    Peter Kwong

    Insert

    SARS-CoV-2-NTD-BA.4/5 (S )
    

    Tags

    AVI, HRV3C, and scFc

    Expression

    Mammalian

    Mutation

    T19I, L24S, P25-, P26-, A27-, H69-, V70-, G142D, …

    Available Since

    Oct. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. human KANK2-A670V Ankyrin Repeats pET151

    Plasmid

    #164055

    Purpose

    Expresses human KANK2 Ank domain - A670V mutant in bacterial cells

    Depositor

    Ben Goult

    Insert

    Kidney Ankyrin Repeat-Containing Protein 2 A670V mutant (KANK2 Human)
    

    Tags

    His-tag, TEV cleavage site

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. mouse KANK3 Ankyrin Repeats pET151

    Plasmid

    #164056

    Purpose

    Expresses mouse KANK3 Ank domain in bacterial cells

    Depositor

    Ben Goult

    Insert

    Kidney Ankyrin Repeat-Containing Protein 3 (Kank3 Mouse)
    

    Tags

    His-tag, TEV cleavage site

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. mouse KANK4 Ankyrin Repeats pET151

    Plasmid

    #164057

    Purpose

    Expresses mouse KANK4 Ank domain in bacterial cells

    Depositor

    Ben Goult

    Insert

    Kidney Ankyrin Repeat-Containing Protein 4 (Kank4 Mouse)
    

    Tags

    His-tag, TEV cleavage site

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. loxP-T2A-Myr-DeadCasp8-ERT2-rox-FNF-loxP-T2A-Myr-Casp8-ERT2-rox

    Plasmid

    #182360

    Purpose

    Shuttle vector for T2A-Myr-DeadCasp8-ERT2 and T2A-Myr-Casp8-ERT2

    Depositor

    Mario Capecchi

    Insert

    Myr-DeadCasp8-ERT2 and Myr-Casp8-ERT2
    

    Use

    Cre/Lox, Mouse Targeting, and Synthetic Biology ;…

    Expression

    Mammalian

    Available Since

    March 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCDZ2FE_Champ1_1-812_2PA

    Plasmid

    #105676

    Purpose

    Expresses Flag-tagged full-length human Champ1 protein with mutations abolishing Rev7 binding

    Depositor

    Thanos Halazonetis

    Insert

    Champ1 (CHAMP1 Human)
    

    Tags

    2X Flag tag

    Expression

    Mammalian

    Mutation

    Mutations: P336A, P341A

    Available Since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pT5T_Rev7_1-211_SA112_KVK_YAK

    Plasmid

    #105630

    Purpose

    Expresses mouse monomeric Rev7 protein with mutations abolishing binding to Champ1 and Rev3

    Depositor

    Thanos Halazonetis

    Insert

    Rev7 (Mad2l2 Mouse)
    

    Expression

    Bacterial

    Mutation

    Mutations: F11S, G12A, I41Y, V132K, C133V, A135K,…

    Promoter

    T7

    Available Since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. MS2_SC2_S_B.1.640

    Plasmid

    #180228

    Purpose

    Generation of MS2 Virus-Like Particles packaged with the sequence for the B.1.640 SARS-CoV-2 S gene (Amino Acid 319-869)

    Depositor

    Paul Freemont

    Inserts

    Maturation Protein
    Coat Protein Dimer
    S gene (S SARS-CoV-2)
    

    Expression

    Bacterial

    Mutation

    B.1.640 (Amino Acid 319-869)

    Promoter

    T7

    Available Since

    Jan. 28, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. CD44U-c010

    Plasmid

    #175453

    Purpose

    Protein expression/secretion in mammalian cells. CD44 splice variant 10 (exons 2-5, 11, 15-16), A20-E649.

    Depositor

    Opher Gileadi

    Insert

    CD44 (CD44 Human)
    

    Tags

    His6

    Expression

    Mammalian

    Available Since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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