We narrowed to 14,241 results for: Cre

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pCIS-krPAM2

Plasmid

#25457

Depositor

Richard Mains

Article

Milgram et al J Cell Biol. 1992 May . 117(4):717-28.

Insert

PAM2 (Pam Rat)

Expression

Mammalian

Mutation

Exon A deleted (exon A extends from nt 1475 to n…

Available Since

July 13, 2010

Availability

Academic Institutions and Nonprofits only
pMX Vav1 Y3F/NSH3* (P651L) uGFP

Plasmid

#14562

Depositor

Joan Brugge

Article

Wilsbacher et al Cell Commun Signal. 2006 May 18;4:5. doi

Insert

Vav1 (VAV1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

Y3F/NSH3* (P651L)

Available Since

April 12, 2007

Availability

Academic Institutions and Nonprofits only
pMX Vav1 Y3F/SH2* (R696K) uGFP

Plasmid

#14565

Depositor

Joan Brugge

Article

Wilsbacher et al Cell Commun Signal. 2006 May 18;4:5. doi

Insert

Vav1 (VAV1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

Y3F/SH2* (R696K)

Available Since

April 12, 2007

Availability

Academic Institutions and Nonprofits only
pMX Vav1 Y3F/CSH3* (P833L) uGFP

Plasmid

#14566

Depositor

Joan Brugge

Article

Wilsbacher et al Cell Commun Signal. 2006 May 18;4:5. doi

Insert

Vav1 (VAV1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

Y3F/CSH3* (P833L)

Available Since

April 12, 2007

Availability

Academic Institutions and Nonprofits only
pIND20-KNL1-rvsf_aaaa-dCas9

Plasmid

#248562

Purpose

A CRISPR-based technology to study chromosome-specific aneuploidy by targeting human centromeres

Depositor

Teresa Davoli

Article

Bosco et al Cell. 2023 Apr 27;186(9):1985-2001.e19.

Insert

KNL1-rvsf_aaaa-dCas9

Use

CRISPR

Tags

HA, 2xNLS

Expression

Mammalian

Mutation

KNL1 RVSF58-61AAAA; Cas9 D10A, H840A

Promoter

TRE, miniCMV

Available Since

May 15, 2026

Availability

Academic Institutions and Nonprofits only

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pHAGE-KNL1-rvsf_aaaa-dCas9

Plasmid

#248567

Purpose

A CRISPR-based technology to study chromosome-specific aneuploidy by targeting human centromeres

Depositor

Teresa Davoli

Article

Bosco et al Cell. 2023 Apr 27;186(9):1985-2001.e19.

Insert

KNL1-rvsf_aaaa-dCas9

Use

CRISPR

Tags

HA, 2xNLS

Expression

Mammalian

Mutation

KNL1 RVSF58-61AAAA; Cas9 D10A, H840A

Promoter

CMV, TetO2

Available Since

Feb. 6, 2026

Availability

Academic Institutions and Nonprofits only

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pAcBac3-EcLeu TAG-EGFP**

Plasmid

#228785

Purpose

EcLeu aaRS/tRNA system for TAG suppression with EGFP containing two stop codons for dual suppression

Depositor

Abhishek Chatterjee

Article

Osgood et al Angew Chem Int Ed Engl. 2023 May 2;62(19

Inserts

EcLeuRS
EcLtR
EGFP**

Use

Baculoviral

Expression

Mammalian

Mutation

TAG suppressor

Available Since

May 7, 2025

Availability

Academic Institutions and Nonprofits only

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Nuc-mTurq2-Apollo-NADP+

Plasmid

#193302

Purpose

Expresses mTurq2-Apollo-NADP+ sensor localized to the nucleus

Depositor

Jonathan Rocheleau

Article

Chang et al ACS Sens. 2022 Oct 21. doi: 10.1021/acss

Insert

G6PD (G6PD Human)

Tags

SV40 NLS and mTurqoise2

Expression

Mammalian

Mutation

S40A, R72Q, H201N, K205T

Promoter

T7

Available Since

Dec. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA GFP10-RHOA

Plasmid

#133068

Purpose

Expresses RHOA GTPase with tripartite split GFP10

Depositor

Stéphanie Cabantous

Article

Koraichi et al J Cell Sci. 2018 Jan 8;131(1). pii: jcs.

Insert

ras homolog family member A (RHOA Human)

Tags

GFP10 (split-GFP tripartite)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pET28-MHL: nsp16_SARS-CoV-2|nsp10_SARS-CoV-2

Plasmid

#167849

Purpose

Bacterial expression of the SARS-CoV-2 proteins nsp10 and nsp16 from a bicistronic mRNA

Depositor

Cheryl Arrowsmith

Article

Perveen et al SLAS Discov. 2021 Jan 10:247255522098504

Insert

nsp10, nsp16

Tags

6xHis-TEV

Expression

Bacterial

Promoter

T7

Available Since

April 22, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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B-SpCas9-HF1

Plasmid

#126762

Purpose

Expression plasmid for human codon-opt. increased fidelity Blackjack-SpCas9-HF1 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than SpCas9HF1.

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

Insert

B-SpCas9-HF1

Use

CRISPR

Tags

3xFLAG and NLS

Expression

Mammalian

Mutation

N497A; R661A; Q695A; Q926A; amino acids 1005-1013…

Promoter

CBh

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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p231-pOsAct1::hpt-pZmUbi::BdPetC-p35S::mTurquoise

Plasmid

#129650

Purpose

Rieske FeS overexpression

Depositor

Susanne von Caemmerer

Article

Ermakova et al Commun Biol. 2019 Aug 16;2:314. doi: 10.

Insert

CDS of PetC gene encoding for the Rieske FeS subunit

Expression

Plant

Mutation

Codon modified for Golden Gate

Promoter

pZmUbi

Available Since

Oct. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-hMbCas12a-NLS(nucleoplasmin)-3xHA (AAS2134)

Plasmid

#114090

Purpose

CAG promoter expression plasmid for human codon optimized MbCas12a nuclease with C-terminal NLS and HA tag

Depositor

Keith Joung, Benjamin Kleinstiver

Article

Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

Insert

human codon optimized MbCas12a

Tags

NLS(nucleoplasmin) and 3xHA

Expression

Mammalian

Available Since

Dec. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pHA-N1-GalT

Plasmid

#87326

Purpose

To express GalT-HA in mammalian cells, GalT encodes the 1-81 amino acids from β1,4-galactosyltransferase

Depositor

Lei Lu

Article

Tie et al Mol Biol Cell. 2016 Jan 13. pii: mbc.E15

Insert

1-81 amino acids from β1,4-galactosyltransferase (B4GALT1 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV-F

Available Since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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Sema3a(S)-AP-His

Plasmid

#72012

Purpose

Expresses the Sema3A protein (truncated at cleavage site P1; ie, short), C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema3a (Sema3a Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 22, 2016

Availability

Academic Institutions and Nonprofits only

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PFB0475c-bio-His

Plasmid

#50822

Purpose

Expresses enzymatically monobiotinylated full length PFB0475c

Depositor

Gavin Wright

Article

Zenonos et al Malar J. 2014 Mar 12;13(1):93. doi: 10.1

Insert

PFB0475c

Tags

ratCD4d3+4,biotinylation sequence, 6xHis

Expression

Mammalian

Mutation

all potential N-linked glycosylation sites (N-X-S…

Promoter

CMV

Available Since

Feb. 13, 2014

Availability

Academic Institutions and Nonprofits only

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PFA0210c-bio-His

Plasmid

#50821

Purpose

Expresses enzymatically monobiotinylated full length PFA0210c

Depositor

Gavin Wright

Article

Zenonos et al Malar J. 2014 Mar 12;13(1):93. doi: 10.1

Insert

PFA0210c

Tags

ratCD4d3+4,biotinylation sequence, 6xHis

Expression

Mammalian

Mutation

all potential N-linked glycosylation sites (N-X-S…

Promoter

CMV

Available Since

Feb. 13, 2014

Availability

Academic Institutions and Nonprofits only

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pTRIP-hPGK-Blast-2A-STING-mNeonGreen

Plasmid

#227186

Purpose

Lentiviral expression plasmid encoding STING-mNeonGreen under hPGK promoter

Depositor

Nir Hacohen

Article

Gentili et al Cell Syst. 2024 Dec 18;15(12):1264-1277.

Insert

STING1 (STING1 Human)

Use

Lentiviral

Tags

mNeonGreen

Expression

Mammalian

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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nuc-yHS1-M7A

Plasmid

#159167

Purpose

Nuclear labile heme reporter

Depositor

Amit Reddi

Article

Hanna et al Proc Natl Acad Sci U S A. 2016 Jul 5;113

Insert

nuc-yHS1-M7A

Tags

SV40 NLS

Expression

Yeast

Mutation

Mutated Met 7 of the cyt b562 module of nuc-yHS1 …

Promoter

GPD

Available Since

Sept. 21, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA-FRT-TO-FH-Nsp8-Nsp7_GC3opt

Plasmid

#157701

Purpose

mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp8-Nsp7 fusion protein under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

FH-Nsp8-Nsp7_GC3opt (ORF1ab SARS-CoV-2)

Tags

Flag-His8

Expression

Mammalian

Mutation

codon optimized; gene expression was optimized by…

Available Since

Aug. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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