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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 22,691 results for: EMB

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Showing: 7601 - 7620 of 22691 results

  3. CD40-bio-His

    Plasmid
    #51648
    Purpose
    Expresses full-length Tumor necrosis factor receptor superfamily member CD40 ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    CD40 molecule (CD40 Human)
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    51648-plasmid-map-sequence-id-78971

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  4. nes374tk/lacZ

    Plasmid
    #47615
    Purpose
    used to create transgenic mice expressing LacZ reporter with Nestin enhancer
    Depositor
    Urban Lendahl
    Article
    Lothian et al Exp Cell Res. 1999 May 1;248(2):509-19.
    Insert
    nestin 2nd intron fragment (NES Human)
    Use
    Enhancer reporter
    Tags
    HSV tk promoter and lacZ
    Mutation
    374 bp from 2nd intron (comprising bp 1153 to 152…
    Promoter
    HSV tk promoter
    Available Since
    Aug. 28, 2013
    Availability
    Academic Institutions and Nonprofits only

  5. InterTag-3xL

    Plasmid
    #186913
    Purpose
    Sleeping Beauty transposon system with constitutive bi-directional promoter, one side expressing SpyTag003-sfCherry11 fused to PDGFR through a (GGGGS)3 linker and the other side expressing BFP and puromycin resistance gene
    Depositor
    Taekjip Ha, Allen Liu
    Article
    Moghimianavval et al Small. 2022 May 26:e2202104. doi: 10.100
    Insert
    SpyTag003-sfCherry11-(GGGGS)3-PDGFR
    Expression
    Mammalian
    Available Since
    Aug. 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    186913-plasmid-map-sequence-id-369408

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  9. pGBW-m4046292

    Plasmid
    #149062
    Purpose
    Yeast Expression plasmid for SARS-CoV-2 M (membrane)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 M (membrane) (M Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable thrombin;StrepII
    Expression
    Yeast
    Mutation
    wt
    Promoter
    pTEF1
    Available Since
    May 14, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    149062-plasmid-map-sequence-id-297979

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  10. pGBW-m4046381

    Plasmid
    #149010
    Purpose
    Yeast Expression plasmid for SARS-CoV-2 M (membrane)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 M (membrane) (M Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable thrombin;3xFLAG
    Expression
    Yeast
    Mutation
    wt
    Promoter
    pTEF1
    Available Since
    May 14, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    149010-plasmid-map-sequence-id-297723

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  11. pACEBac1-PSMB1-PSMB2-PSMB3-PSMB4-TEV-2xSTII-PSMB5-PSMB6-PSMB7

    Plasmid
    #214140
    Purpose
    Insect cell expression of human 20S CP beta-subunits with a c-terminal twin-strep-tag on beta7
    Depositor
    Brenda Schulman
    Article
    Adolf et al Nat Struct Mol Biol. 2024 Apr 10. doi: 1
    Inserts
    PSMB1 (PSMB1 Human)
    PSMB2 (PSMB2 Human)
    PSMB3 (PSMB3 Human)
    PSMB4 (PSMB4 Human)
    PSMB5 (PSMB5 Human)
    PSMB6 (PSMB6 Human)
    PSMB7 (PSMB7 Human)
    Tags
    2xSTII
    Expression
    Insect
    Promoter
    polyhedrin
    Available Since
    May 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    214140-plasmid-map-sequence-id-431241

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  12. pSUC2::GNSI (NrsR)

    Plasmid
    #121537
    Purpose
    This plasmid contains a CYC1pr driven GNSI reporter and flanking homology to SUC2. Digestion with NotI, SacI, and EcoRV, allows integration at the SUC2 locus.
    Depositor
    Alexey Merz
    Article
    Plemel et al Traffic. 2020 Nov 23. doi: 10.1111/tra.1
    Inserts
    CYC1 Promoter (CYC1 Budding Yeast)
    Nyv1 Cytosolic Domain (aa 6-230)
    Snc1 Transmembrane Domain (TMD)
    SUC2 CDS
    SUC2 terminator
    NrsR
    Tags
    mGFP5
    Expression
    Yeast
    Mutation
    M109I (Naturally occurring SNP) and The first 21 …
    Available Since
    Feb. 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    121537-plasmid-map-sequence-id-235299

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Showing: 7601 - 7620 of 22691 results