We narrowed to 17,340 results for: EMB

1. pME-puro-CLIP-FLAG-CD59

   Plasmid

   #50375

   Purpose

   Express CLIP and FLAG-tagged human CD59, a GPI-AP in mammmalian cells

   Depositor

   Reika Watanabe

   Article

   Castillon et al Mol Biol Cell. 2013 Jun;24(12):2021-33.

   Insert

   CLIP
   

   Expression

   Mammalian

   Promoter

   SR alpha

   Available Since

   July 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMaCTag-Z33

   Plasmid

   #120050

   Purpose

   Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with FLAG

   Depositor

   Michael Knop

   Article

   Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   FLAG
   

   Use

   Pcr template

   Tags

   FLAG

   Promoter

   None

   Available Since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET SUMO-FL-syt1

   Plasmid

   #213628

   Purpose

   Bacterial expression of SUMO-tagged rat FL-syt1

   Depositor

   Edwin Chapman

   Article

   Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

   Insert

   FL-syt1 (Syt1 Rat)
   

   Tags

   SUMO

   Expression

   Bacterial

   Available Since

   March 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. TAL-L1-Tf1-Ctrl

   Plasmid

   #103036

   Purpose

   Plasmid for IvT reaction with T3. Encodes LINE-1 specific TALE sequence followed by multi cloning site

   Depositor

   Maria-Elena Torres-Padilla

   Article

   Jachowicz et al Nat Genet. 2017 Aug 28. doi: 10.1038/ng.

   Insert

   TAL-L1-Tf1
   

   Use

   T3 in vitro transcription for expression in mamma…

   Tags

   FLAG and HA

   Available Since

   Nov. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pV2A-T

   Plasmid

   #153022

   Purpose

   Single-plasmid multi-gene expression system suitable for fungi. The obtained polycistronic gene is expressed under the control of a TetON inducible promoter

   Depositor

   Vito Valiante

   Article

   Hoefgen et al Metab Eng. 2018 Jul;48:44-51. doi: 10.10

   Type

   Empty backbone

   Expression

   Bacterial and Yeast

   Available Since

   Oct. 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. MSCV Puro-EFS:GFP

   Plasmid

   #68484

   Purpose

   Retrovirus expressing GFP driven by EFS promoter

   Depositor

   Tyler Jacks

   Article

   Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

   Inserts

   Puromycin Resistance
   GFP
   

   Use

   Retroviral

   Expression

   Mammalian

   Promoter

   EFS and LTR

   Available Since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. BcLOV-mCh-SOS

   Plasmid

   #208279

   Purpose

   Expresses BcLOV-SOS for optogenetic activation of Ras/Erk signaling. Includes an mCherry tag.

   Depositor

   Lukasz Bugaj

   Article

   Pal et al Proc Natl Acad Sci U S A. 2023 Aug 8;120

   Insert

   BcLOV-mCh-SOS
   

   Use

   Lentiviral

   Promoter

   SFFV

   Available Since

   Jan. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTK38_mCherry-LGN-C

   Plasmid

   #46346

   Depositor

   Iain Cheeseman

   Article

   Kiyomitsu et al Cell. 2013 Jul 18;154(2):391-402. doi: 1

   Insert

   LGN C-terminal region (GPSM2 Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   contains aa365-684; deletion of S524

   Promoter

   CMV

   Available Since

   Aug. 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMaCTag-P24

   Plasmid

   #120035

   Purpose

   Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with TAP

   Depositor

   Michael Knop

   Article

   Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   TAP
   

   Use

   Pcr template

   Tags

   TAP

   Promoter

   None

   Available Since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBTK001

    Plasmid

    #110573

    Purpose

    Broad-host-range toolkit (BTK) entry vector, with superfolder GFP

    Depositor

    Jeffrey Barrick

    Article

    Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

    Type

    Empty backbone

    Use

    Synthetic Biology

    Available Since

    Feb. 20, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pSicoR-mCh-GFPi

    Plasmid

    #21905

    Depositor

    Miguel Ramalho-Santos

    Article

    Gaspar-Maia et al Nature. 2009 Aug 13;460(7257):863-8. doi

    Insert

    GFPi
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    Sept. 30, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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12. CBla_2.0_EmrE_TMD4_3P

    Plasmid

    #207848

    Purpose

    BLaTM-System; Antiparallel positive control EmrE_TMD4_3P in CBLa 2.0 vector

    Depositor

    Dieter Langosch

    Article

    Julius et al J Mol Biol. 2017 Apr 18. pii: S0022-2836

    Insert

    C-BLa 2.0 fusion protein
    

    Tags

    Flag (DYKDHDG)

    Expression

    Bacterial

    Available Since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. TOPO CCSP

    Plasmid

    #68453

    Purpose

    TOPO construct expressing CCSP for generation of GMAP-compatible promoter

    Depositor

    Tyler Jacks

    Article

    Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

    Insert

    Clara Cell Secretory Protein Promoter
    

    Use

    Gmap

    Expression

    Bacterial

    Promoter

    Plac

    Available Since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMaCTag-P10

    Plasmid

    #120021

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mKOk

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    mKOk
    

    Use

    Pcr template

    Tags

    mKOk

    Promoter

    None

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAG-mWasabi-ER

    Plasmid

    #164165

    Purpose

    Expresses mWasabi fused with an ER-targeting sequence of calreticulin and the ER retrieval sequence, KDEL, under the control of CAG promoter

    Depositor

    Isei Tanida

    Article

    Tanida et al Sci Rep. 2020 Dec 14;10(1):21871. doi: 1

    Insert

    mWasabi
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Feb. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMaCTag-P18

    Plasmid

    #120029

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mKeima8.5

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    mKeima8.5
    

    Use

    Pcr template

    Tags

    mKeima8.5

    Promoter

    None

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pNS3

    Plasmid

    #131770

    Purpose

    PADH1_FUSN_Fusion Red_Cry2Olig_TACT1 for Integration into δ-sites

    Depositor

    Jared Toettcher

    Article

    Zhao et al Nat Chem Biol. 2019 Jun;15(6):589-597. d

    Insert

    PADH1_FUSN_Fusion Red_Cry2Olig_TACT1
    

    Use

    Integration into δ-sites

    Expression

    Yeast

    Mutation

    E490G to make Cry2 into Cry2Olig

    Promoter

    PADH1

    Available Since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. TAL2384

    Plasmid

    #36793

    Depositor

    Keith Joung

    Article

    Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

    Insert

    BRCA1-TALEN_Left (Brca1 Human)
    

    Use

    Mammalian, t7 for in vitro mrna synthesis

    Tags

    3X Flag and WT FOKI

    Promoter

    CMV

    Available Since

    June 26, 2012

    Availability

    Academic Institutions and Nonprofits only

19. pGEMHE-mClover3

    Plasmid

    #171497

    Purpose

    T7 promotor drives in vitro transcription of mClover3 mRNA

    Depositor

    Melina Schuh

    Article

    Cavazza et al Cell. 2021 Apr 30. pii: S0092-8674(21)00

    Insert

    mClover3
    

    Use

    Pgehme

    Promoter

    T7

    Available Since

    Aug. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pUC19-Split-Broccoli-Toehold-Switch

    Plasmid

    #87316

    Purpose

    Expresses the Split-Broccoli system and a Trigger/Toehold switch for mCherry.

    Depositor

    Donald Burke

    Article

    Alam et al ACS Synth Biol. 2017 May 26. doi: 10.102

    Insert

    Split-Broccoli system and RNA Trigger (TS2_AT01) Toehold (TS2_KS01) switch for mCherry
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    P70a

    Available Since

    May 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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