We narrowed to 166,397 results for: addgene

1. pXPR_047

   Plasmid

   #107145

   Purpose

   measures Cas9 activity

   Depositor

   David Root

   Insert

   GFP + sgRNA for GFP
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   March 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMal-Strep

   Plasmid

   #209345

   Purpose

   Protein expression vector with a TEV cleavable N-terminal 6xHis-MBP and C-terminal StrepTagII

   Depositor

   Lucia Strader

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   Jan. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pXPR_118

   Plasmid

   #113667

   Purpose

   Lentiviral vector expressing dCas9

   Depositor

   John Doench

   Insert

   dCas9
   

   Use

   CRISPR and Lentiviral

   Mutation

   catalytically inactive

   Available Since

   Sept. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRS426 TEF mCherry

   Plasmid

   #129433

   Purpose

   express mcherry

   Depositor

   Oskar Laur

   Insert

   mcherry
   

   Expression

   Yeast

   Available Since

   March 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pOpen-T4 PNK

   Plasmid

   #165520

   Purpose

   The most commonly used polynucleotide kinase.

   Depositor

   Drew Endy, Jennifer Molloy, FreeGenes Project

   Insert

   T4 PNK
   

   Use

   Synthetic Biology

   Available Since

   Aug. 3, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pET28a(+)/FbGH17B

   Plasmid

   #100912

   Purpose

   Expression of the Beta-1,3-Exo-Laminarinase FbGH17B in E.coli

   Depositor

   Jan-Hendrik Hehemann

   Insert

   FbGH17Bcat
   

   Tags

   His-Tag

   Expression

   Bacterial

   Mutation

   not full length

   Available Since

   April 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1-ccdB-Nanoluc

   Plasmid

   #87067

   Purpose

   Mammalian Gateway-compatible expression vector backbone with a C-terminal Nanoluc luciferase

   Depositor

   Mikko Taipale

   Type

   Empty backbone

   Tags

   Nanoluc luciferase

   Expression

   Mammalian

   Available Since

   March 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSG5 Large T

   Plasmid

   #9053

   Depositor

   William Hahn

   Insert

   large T antigen (SV40gp6 simian virus)
   

   Expression

   Mammalian

   Available Since

   Jan. 5, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLionII

   Plasmid

   #1730

   Purpose

   This is a 3rd generation lentiviral transfer vector (slightly lower titer, highest expression level) from Nolan lab's FELIX vector system

   Depositor

   Garry Nolan

   Type

   Empty backbone

   Use

   Lentiviral

   Available Since

   Dec. 7, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti.CAG.H2B-Dendra2.W

    Plasmid

    #51005

    Purpose

    Lentivector that expresses H2B-Dendra2 from internal CAG enhancer-promoter

    Depositor

    Rusty Lansford

    Insert

    H2B-Dendra2
    

    Use

    Lentiviral

    Tags

    histone2B

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    June 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHIV-Luciferase

    Plasmid

    #21375

    Purpose

    self-inactivating 3rd generation lentiviral plasmid for co-expression of your gene of interest and Luciferase

    Depositor

    Bryan Welm

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    June 19, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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12. AbVec2.0-IGHG4

    Plasmid

    #99578

    Purpose

    Expression of secretory immunoglobulin heavy chains in mammalian cells, human IgG4 isotype

    Depositor

    Hedda Wardemann

    Type

    Empty backbone

    Expression

    Mammalian

    Promoter

    hCMV IE1 gene promoter

    Available Since

    Aug. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1-Nanoluc-ccdB

    Plasmid

    #87070

    Purpose

    Mammalian Gateway-compatible expression vector backbone with an N-terminal Nanoluc luciferase

    Depositor

    Mikko Taipale

    Type

    Empty backbone

    Tags

    Nanoluc luciferase

    Expression

    Mammalian

    Available Since

    Aug. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28a(+)/FbGH17A

    Plasmid

    #100911

    Purpose

    Expression of the Beta-1,3-Endo-Laminarinase FbGH17A in E.coli

    Depositor

    Jan-Hendrik Hehemann

    Insert

    FbGH17A
    

    Tags

    His-Tag

    Expression

    Bacterial

    Available Since

    March 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. LHZ1493

    Plasmid

    #222102

    Purpose

    Cloning backbone for sgRNA in Kluyveromyces marxianus. Expresses highly specific SlugCas9.

    Depositor

    Yongming Wang

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    Aug. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV_Ef1a_DIO_hTyrNull_HATag

    Plasmid

    #205285

    Purpose

    Truncated version of human Tyrosinase as control virus for Tyrosinase expression

    Depositor

    Matthias Prigge

    Insert

    hTyrosinase (truncated)
    

    Use

    AAV

    Available Since

    Dec. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMF440

    Plasmid

    #62550

    Purpose

    Broad host range plasmid for constitutive expression of mCherry. Designed for expression in Pseudomonas aeruginosa.

    Depositor

    Michael Franklin

    Insert

    mCherry
    

    Expression

    Bacterial

    Promoter

    trc

    Available Since

    April 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTfR-mTurquoise2

    Plasmid

    #112953

    Purpose

    In vivo visualization of Transferrin receptors (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Insert

    pTfR
    

    Tags

    mTurquoise2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. AbVec2.0-IGHG2

    Plasmid

    #99576

    Purpose

    Expression of secretory immunoglobulin heavy chains in mammalian cells, human IgG2 isotype

    Depositor

    Hedda Wardemann

    Type

    Empty backbone

    Expression

    Mammalian

    Promoter

    hCMV IE1 gene promoter

    Available Since

    Aug. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSilencer-Opa1 KO

    Plasmid

    #191949

    Purpose

    Expression of a siRNA that targets Opa1

    Depositor

    Luca Scorrano

    Insert

    Opa1
    

    Expression

    Mammalian

    Available Since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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