Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 84,770 results for: ell

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AiP14427 - pAAV-AiE1190m-minBG-SYFP2-P2A-3XFLAG-10aa-H2B-WPRE3-BGHpA (Alias: CN4427)

Plasmid

#224181

Purpose

AiE1190m is an enhancer sequence, designed to drive AAV-mediated transgene expression in striatal D2 MSN>D1 MSN

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Insert

SYFP2-P2A-3XFLAG-10aa-H2B

Use

AAV

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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AiP14425 - pAAV-AiE1180h-minBG-SYFP2-P2A-3XFLAG-10aa-H2B-WPRE3-BGHpA (Alias: CN4425)

Plasmid

#224180

Purpose

AiE1180h is an enhancer sequence, designed to drive AAV-mediated transgene expression in striatal cholinergic neurons (weak)

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Insert

SYFP2-P2A-3XFLAG-10aa-H2B

Use

AAV

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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AiP14428 - pAAV-AiE1191m-minBG-SYFP2-P2A-3XFLAG-10aa-H2B-WPRE3-BGHpA (Alias: CN4428)

Plasmid

#224182

Purpose

AiE1191m is an enhancer sequence, designed to drive AAV-mediated transgene expression in striatal MSNs

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Insert

SYFP2-P2A-3XFLAG-10aa-H2B

Use

AAV

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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AiP15356 - pAAV-AiE0784m-minBG-DIO-CoChR-EGFP-WPRE3-BGHpA (Alias: CN5356)

Plasmid

#224196

Purpose

AiE0784m is an enhancer sequence, designed to drive Cre dependent AAV-mediated transgene expression in striatal MSNs

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Insert

CoChR-EGFP

Use

AAV

Available Since

Sept. 25, 2024

Availability

Academic Institutions and Nonprofits only

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AiP15049 - pAAV-AiE1425m-minBG-SYFP2-P2A-3XFLAG-10aa-H2B-WPRE3-BGHpA (Alias: CN5049)

Plasmid

#224191

Purpose

AiE1425m is an enhancer sequence, designed to drive AAV-mediated transgene expression in striatal Sst_Chodl neurons

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Insert

SYFP2-P2A-3XFLAG-10aa-H2B

Use

AAV

Available Since

Sept. 25, 2024

Availability

Academic Institutions and Nonprofits only

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AiP15045 - pAAV-AiE1421m-minBG-SYFP2-P2A-3XFLAG-10aa-H2B-WPRE3-BGHpA (Alias: CN5045)

Plasmid

#224190

Purpose

AiE1421m is an enhancer sequence, designed to drive AAV-mediated transgene expression in striatal Pvalb+/Pthlh+ neurons

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Insert

SYFP2-P2A-3XFLAG-10aa-H2B

Use

AAV

Available Since

Sept. 25, 2024

Availability

Academic Institutions and Nonprofits only

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AiP15042 - pAAV-AiE1418m-minBG-SYFP2-P2A-3XFLAG-10aa-H2B-WPRE3-BGHpA (Alias: CN5042)

Plasmid

#224188

Purpose

AiE1418m is an enhancer sequence, designed to drive AAV-mediated transgene expression in striatal Pvalb+/Pthlh+ neurons

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Insert

SYFP2-P2A-3XFLAG-10aa-H2B

Use

AAV

Available Since

Sept. 25, 2024

Availability

Academic Institutions and Nonprofits only

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AiP15041 - pAAV-AiE1417m-minBG-SYFP2-P2A-3XFLAG-10aa-H2B-WPRE3-BGHpA (Alias: CN5041)

Plasmid

#224187

Purpose

AiE1417m is an enhancer sequence, designed to drive AAV-mediated transgene expression in striatal Pvalb+/Pthlh+ neurons

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Insert

SYFP2-P2A-3XFLAG-10aa-H2B

Use

AAV

Available Since

Sept. 25, 2024

Availability

Academic Institutions and Nonprofits only

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AiP15140: pAAV-AiE0873m_3xC2-minBG-CoChR-EGFP-WPRE3-BGHpA (Alias: CN5140)

Plasmid

#214869

Purpose

Expression of CoChR-EGFP in striatal cholinergic neurons

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Has Service

AAV PHP.eB

Insert

CoChR-EGFP

Use

AAV

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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AiP13981: pAAV-AiE0621h-minBG-iCre(R297T)-BGHpA (Alias: CN3981)

Plasmid

#214849

Purpose

Expression of iCre(R297T) in olfactory tubercle mixed neuron populations

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Insert

iCre(R297T)

Use

AAV

Mutation

R297T

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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AiP13754 - pAAV-AiE0779m_3xC2-minBG-mTFP1-WPRE3-BGHpA (Alias: CN3754)

Plasmid

#191733

Purpose

AI plasmid ALIAS: AiP13754. Expression of mTFP1 (monomeric teal fluorescent protein) in striatal direct pathway medium spiny neurons (D1-MSNs)

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Insert

mTFP1

Use

AAV

Mutation

none

Available Since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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Twinkle-APEX2-V5

Plasmid

#129705

Purpose

APEX2 fused to the mitochondrial nucleoid protein Twinkle

Depositor

Alice Ting

Article

Han et al Cell Chem Biol. 2017 Mar 16;24(3):404-41

Insert

Twinkle-APEX2-V5 (TWNK, APEX2 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV promoter

Available Since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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MEK1 (S218D,S222D)-pcw107

Plasmid

#64604

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

MAP2K1 (MAP2K1 Human)

Use

Lentiviral

Mutation

S218D, S222D

Promoter

PGK

Available Since

June 18, 2015

Availability

Academic Institutions and Nonprofits only

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beta-catenin (S33Y)-pcw107

Plasmid

#64615

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

CTNNB1 (CTNNB1 Human)

Use

Lentiviral

Mutation

S33Y

Promoter

PGK

Available Since

May 12, 2015

Availability

Academic Institutions and Nonprofits only

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pLenti-PGK-EGFP-ALDH1A1-Neo

Plasmid

#189742

Purpose

The construct was used to express GFP tagged human ALDH1A1 in mammalian cells for the analysis of the subcellular location of this protein.

Depositor

Robert Sobol

Article

Li et al Commun Biol. 2021 Dec 21;4(1):1420. doi:

Insert

ALDH1A1 (ALDH1A1 Human)

Tags

EGFP

Expression

Mammalian

Promoter

PGK

Available Since

Oct. 19, 2022

Availability

Academic Institutions and Nonprofits only

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AP1muA-Halo-ALFA-polyA-G418 HR

Plasmid

#229681

Purpose

Homology repair plasmid for endogenous tagging of AP1muA at the C-terminus with HaloTag and an ALFA tag. Contains a G418 resistance cassette for selection of edited cells.

Depositor

Francesca Bottanelli

Article

Stockhammer et al Nat Cell Biol. 2024 Oct 4. doi: 10.1038/

Insert

genomic sequence AP1M1 locus (AP1M1 Human)

Use

CRISPR

Tags

Halo, ALFA

Available Since

Jan. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-PGK-EGFP-ALDH1A2-Neo

Plasmid

#189743

Purpose

The construct was used to express GFP tagged human ALDH1A2 in mammalian cells for the analysis of the subcellular location of this protein.

Depositor

Robert Sobol

Article

Li et al Commun Biol. 2021 Dec 21;4(1):1420. doi:

Insert

ALDH1A2 (ALDH1A2 Human)

Tags

EGFP

Expression

Mammalian

Promoter

PGK

Available Since

Oct. 19, 2022

Availability

Academic Institutions and Nonprofits only

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Lenti-CMV-PEmax-IRES-ZeoR

Plasmid

#207353

Purpose

Lentiviral transfer plasmid containing CMV-driven expression cassette encoding PEmax enzyme for prime editing.

Depositor

Marianne Carlon

Article

Bulcaen et al Cell Rep Med. 2024 May 21;5(5):101544. d

Insert

Prime editor max (PEmax) enzyme

Use

Lentiviral

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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pTRE-BI-hGRAD

Plasmid

#207837

Purpose

Inducible hGRAD Plasmids for rapid depletion of GFP-tagged proteins in mammalian cells

Depositor

Michaela Mueller-McNicoll

Article

Arnold et al J Cell Biol. 2024 Feb 5;223(2):e20230403

Inserts

mCherry
hFBXW11 (FBXW11 Human)
VHH-GFP4

Expression

Mammalian

Mutation

215 amino acids of the full length protein

Promoter

TRE3G BI promoter

Available Since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pGL3-noSV40-EC1.45-human_min1-mouse_min2

Plasmid

#173965

Purpose

Firefly luciferase enhancer reporter plasmid.

Depositor

Joanna Wysocka

Article

Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

Insert

Human minimal enhancer region 1 (min1) and mouse minimal enhancer region 2 (min2) from SOX9 enhancer cluster EC1.45

Use

Luciferase

Mutation

None

Promoter

SV40 promoter

Available Since

Sept. 1, 2021

Availability

Academic Institutions and Nonprofits only

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