We narrowed to 7,972 results for: Lif
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Plasmid#217635PurposeAAV backbone for sgRNA expression. Contains a Lox-flanked handle sequence for Cre-dependent PCR amplification of sgRNAs. Protospacer is cloned between BstXI and BlpI.DepositorInsertmU6-sgRNA, Handle sequence, EF1a-mTagBFP2
UseAAVPromoterEF1alpha and mU6Available SinceOct. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_hHNF4alpha
Plasmid#206878PurposeThe ORF of HNF4α (variant 2) cDNA amplified from the RT human liver RNA was cloned at the Bam HI/XbaI sites of the pcDNA3.1(+), the mammalian expression vector.DepositorAvailable SinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSET-RCaMP1h
Plasmid#42874Purposea single-wavelength red-shifted genetically encoded calcium indicator constructed from calmodulin and cp-mRubyDepositorInsertRed Fluorescent Calcium binding Protein
TagsEK (Enterokinase) Recognition Site, His Tag (6x),…ExpressionBacterialPromoterT7Available SinceFeb. 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.0-Magneto2.0-p2A-mCherry
Plasmid#74308PurposeBicistronic expression of Magneto2.0 and mCherry in mammalian cellsDepositorTagsFlag and p2A-mCherryExpressionMammalianMutationTRPV4: delta 760–871PromoterCMVAvailable SinceMay 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
Venus1-WTSTAT3
Plasmid#123164PurposeExpresses wild type STAT3 fused to the 1-157 fragment of Venus in mammalian cellsDepositorInsertSTAT3 tagged with Venus1 (aa 1-157) (STAT3 Human)
TagsVenus1 (aa 1-158) for bimolecular fluorescence co…ExpressionMammalianMutationnonePromoterpCMVAvailable SinceAug. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.3-Nm-dCas9-p300 Core
Plasmid#61365Purposeencodes N. meningiditis dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664) driven by CMV promoterDepositorInsertNm-dCas9-p300 Core (EP300 Human, Neisseria meningiditis, Synthetic)
UseCRISPR and Synthetic BiologyTagsHA tag and NLSExpressionMammalianMutationD16A, D587A, H588A, N611A in Nm Cas9PromoterCMVAvailable SinceApril 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
Venus2-WTSTAT3
Plasmid#123165PurposeExpresses wild type STAT3 fused to the 158-238 fragment of Venus in mammalian cellsDepositorInsertSTAT3 tagged with Venus2 (aa 158-238) (STAT3 Human)
TagsVenus2 (aa 158-238) for bimolecular fluorescence …ExpressionMammalianMutationnoneAvailable SinceAug. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
Venus2-Y705F/S727A-STAT3
Plasmid#123177PurposeExpresses Y705F/S727A STAT3 double mutant fused to the 158-238 fragment of Venus in mammalian cellsDepositorInsertSTAT3 tagged with Venus2 (aa 158-238) (STAT3 Human)
TagsVenus2 (aa 158-238) for bimolecular fluorescence …ExpressionMammalianMutationDouble Y705F and S727A substitutionsAvailable SinceAug. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
Venus1-S727A-STAT3
Plasmid#123174PurposeExpresses S727A STAT3 fused to the 1-157 fragment of Venus in mammalian cellsDepositorInsertSTAT3 tagged with Venus1 (aa 1-157) (STAT3 Human)
TagsVenus1 (aa 1-158) for bimolecular fluorescence co…ExpressionMammalianMutationS727A substitutionAvailable SinceAug. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
Venus1-Y705F/S727A-STAT3
Plasmid#123176PurposeExpresses Y705F/S727A STAT3 double mutant fused to the 1-157 fragment of Venus in mammalian cellsDepositorInsertSTAT3 tagged with Venus1 (aa 1-157) (STAT3 Human)
TagsVenus1 (aa 1-158) for bimolecular fluorescence co…ExpressionMammalianMutationDouble Y705F and S727A substitutionsAvailable SinceAug. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
Venus1-Y705F-STAT3
Plasmid#123172PurposeExpresses Y705F STAT3 fused to the 1-157 fragment of Venus in mammalian cellsDepositorInsertSTAT3 tagged with Venus1 (aa 1-157) (STAT3 Human)
TagsVenus1 (aa 1-158) for bimolecular fluorescence co…ExpressionMammalianMutationY705F substitutionAvailable SinceAug. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
Venus1-K685R-STAT3
Plasmid#123170PurposeExpresses K658R STAT3 fused to the 1-157 fragment of Venus in mammalian cellsDepositorInsertSTAT3 tagged with Venus1 (aa 1-157) (STAT3 Human)
TagsVenus1 (aa 1-158) for bimolecular fluorescence co…ExpressionMammalianMutationK685R substitutionAvailable SinceAug. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
Venus2-Y705F-STAT3
Plasmid#123173PurposeExpresses Y705F STAT3 fused to the 158-238 fragment of Venus in mammalian cellsDepositorInsertSTAT3 tagged with Venus2 (aa 158-238) (STAT3 Human)
TagsVenus2 (aa 158-238) for bimolecular fluorescence …ExpressionMammalianMutationY705F substitutionAvailable SinceAug. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
Venus2-S727A-STAT3
Plasmid#123175PurposeExpresses S727A STAT3 fused to the 158-238 fragment of Venus in mammalian cellsDepositorInsertSTAT3 tagged with Venus2 (aa 158-238) (STAT3 Human)
TagsVenus2 (aa 158-238) for bimolecular fluorescence …ExpressionMammalianMutationS727A substitutionAvailable SinceAug. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
Venus1-K49R-STAT3
Plasmid#123166PurposeExpresses K49R STAT3 fused to the 1-157 fragment of Venus in mammalian cellsDepositorInsertSTAT3 tagged with Venus1 (aa 1-157) (STAT3 Human)
TagsVenus1 (aa 1-158) for bimolecular fluorescence co…ExpressionMammalianMutationK49R substitutionAvailable SinceAug. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
Venus2-K49R-STAT3
Plasmid#123167PurposeExpresses K49R STAT3 fused to the 158-238 fragment of Venus in mammalian cellsDepositorInsertSTAT3 tagged with Venus2 (aa 158-238) (STAT3 Human)
TagsVenus2 (aa 158-238) for bimolecular fluorescence …ExpressionMammalianMutationK49R substitutionAvailable SinceAug. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
Venus1-K140R-STAT3
Plasmid#123168PurposeExpresses K140R STAT3 fused to the 1-157 fragment of Venus in mammalian cellsDepositorInsertSTAT3 tagged with Venus1 (aa 1-157) (STAT3 Human)
TagsVenus1 (aa 1-158) for bimolecular fluorescence co…ExpressionMammalianMutationK140R substitutionAvailable SinceAug. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
Venus2-K140R-STAT3
Plasmid#123169PurposeExpresses K140R STAT3 fused to the 158-238 fragment of Venus in mammalian cellsDepositorInsertSTAT3 tagged with Venus2 (aa 158-238) (STAT3 Human)
TagsVenus2 (aa 158-238) for bimolecular fluorescence …ExpressionMammalianMutationK140R substitutionAvailable SinceAug. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
Venus2-K685R-STAT3
Plasmid#123171PurposeExpresses K695R STAT3 fused to the 158-238 fragment of Venus in mammalian cellsDepositorInsertSTAT3 tagged with Venus2 (aa 158-238) (STAT3 Human)
TagsVenus2 (aa 158-238) for bimolecular fluorescence …ExpressionMammalianMutationK685R substitutionAvailable SinceAug. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
GFP Cav2.2 human HAgHA -plus exon 18a - LONG-Cterm in pcDNA3
Plasmid#198451PurposeN-terminal GFP tagged, exofacial HAgHA tag in Domain II, contains exon 18a- Long form of alternatively spliced C-teminus, in pcDNA3 vectorDepositorInsertCav2.2 (CACNA1B Human)
TagsN-terminal GFP, internal double HAExpressionMammalianPromoterCMVAvailable SinceApril 1, 2025AvailabilityAcademic Institutions and Nonprofits only