We narrowed to 8,727 results for: sgRNA

1. API5-CRISPR

   Plasmid

   #157664

   Purpose

   Expresses Cas9 and sgRNA targeting API5

   Depositor

   Byung Il Lee

   Article

   Bong et al Nucleic Acids Res. 2020 Jun 19;48(11):63

   Insert

   Apoptosis inhibitor 5 (API5 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPN187

   Plasmid

   #91574

   Purpose

   Express sgRNA targeting human AKT3

   Depositor

   Lindy Barrett

   Article

   Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

   Insert

   AKT3 (AKT3 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   Oct. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCW57.1 tTA SDHA

   Plasmid

   #177976

   Purpose

   All-in-one dox-repressible expression of cDNA

   Depositor

   Jessica Spinelli, David Sabatini

   Article

   Spinelli et al Science. 2021 Dec 3;374(6572):1227-1237.

   Insert

   SDHA (SDHA Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Silent point mutations to SDHA sgRNA target

   Promoter

   Available since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. ShaCas9

   Plasmid

   #163794

   Purpose

   Expresses ShaCas9, and cloning backbone for sgRNA

   Depositor

   Yongming Wang

   Article

   Hu et al Nucleic Acids Res. 2021 Mar 15. pii: 617

   Type

   Empty backbone

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. LentiCRISPR v2-sgAMPK alpha1 clone3

   Plasmid

   #162121

   Purpose

   Lentiviral sgRNA plasmid targeting human AMPK alpha1

   Depositor

   Boyi Gan

   Article

   Lee et al Nat Cell Biol. 2020 Feb;22(2):225-234. d

   Insert

   sgAMPK alpha1 (PRKAA1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. ARB365

   Plasmid

   #124048

   Purpose

   Encodes pEF1a driving expression of TET3G P2A iRFP713, pEF1a expressing sadCas9 fused to mRuby2 P2A mRuby2, and an sgRNA targeting pUAS expressing mAzamiGreen in a PiggyBac destination vector

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   PB_pEF1a-tet3G-P2A-iRFP713_pEF1a-sadCAS9::mRuby2-P2A-mRuby2-SV40PA_mu6-sgUAS_pUAS-NLS::mAzamiGreen-rgPA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAWp28-v1 scaffold

   Plasmid

   #157976

   Purpose

   pBT264-U6p-{2xBbsI}-v1 sgRNA scaffold-{MfeI} Note: This plasmid is unstable. Screen multiple colonies to identify full length clones.

   Depositor

   Alan Siu Lun Wong

   Article

   Zhou et al Cell Rep. 2020 Aug 11;32(6):108020. doi:

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAWp102-v2 scaffold

   Plasmid

   #157978

   Purpose

   pBT264-mutmU6p-{2xBbsI}-v2 sgRNA scaffold-{MfeI}

   Depositor

   Alan Siu Lun Wong

   Article

   Zhou et al Cell Rep. 2020 Aug 11;32(6):108020. doi:

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAVsc.U6-sgGTTG.CMV/CB-Gluc

   Plasmid

   #121512

   Purpose

   Expresses sgRNA targeting the intron in the MADM alleles (GT and TG).

   Depositor

   Guangping Gao

   Article

   Wang et al Nat Biotechnol. 2018 Oct;36(9):839-842.

   Insert

   sgGTTG
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   July 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pENTR221-H1-sgGFP1-7SK-sgCas9

    Plasmid

    #87908

    Purpose

    multiple sgRNA

    Depositor

    Nicole Déglon

    Article

    Merienne et al Cell Rep. 2017 Sep 19;20(12):2980-2991.

    Insert

    sgGFP1, sgCas9
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    H1, 7SK

    Available since

    Sept. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGL3-U6-pegRNA_HOXD13_CtoT-EGFP

    Plasmid

    #159792

    Purpose

    S. pyogenes pegRNA for C to T mutation at the HOXD13 site of mouse cells using prime editing

    Depositor

    Xiaolong Wang

    Article

    Liu et al Cell Discov. 2020 Apr 28;6:27. doi: 10.1

    Insert

    spacer of pegRNA_HOXD13_CtoT targeting HOXD13 gene (HOXD13 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGL3-U6-pegRNA_HOXD13_CtoG-EGFP

    Plasmid

    #159791

    Purpose

    S. pyogenes pegRNA for C to G mutation at the HOXD13 site of mouse cells using prime editing

    Depositor

    Xiaolong Wang

    Article

    Liu et al Cell Discov. 2020 Apr 28;6:27. doi: 10.1

    Insert

    spacer of pegRNA_HOXD13_CtoG targeting HOXD13 gene (HOXD13 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti-Puro-sgSIK3-A

    Plasmid

    #138682

    Purpose

    Expresses a mouse SIK3-targeting sgRNA and Cas9

    Depositor

    Reuben Shaw

    Article

    Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

    Insert

    sgSIK3 mouse (Sik3 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    Feb. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti-Puro-sgSIK3-C

    Plasmid

    #138683

    Purpose

    Expresses a mouse SIK3-targeting sgRNA and Cas9

    Depositor

    Reuben Shaw

    Article

    Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

    Insert

    sgSIK3 mouse (Sik3 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    Feb. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTBL649 CHYRON2 integration construct

    Plasmid

    #126446

    Purpose

    To integrate the CHYRON2 locus at AAVS1 in human cells.

    Depositor

    Chang Liu

    Article

    Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

    Inserts

    pU6-CHYRON2 hgRNA
    pCMV-puro
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    The SpCas9 sgRNA constant region is mutated to ma…

    Promoter

    CMV and human U6

    Available since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. PB-U6-CNCB_sgSOX2

    Plasmid

    #242175

    Purpose

    A piggybac-based vector containing mouse U6 promoter-driven SOX2 sgRNA and CAG promoter-driven nuclear-localized Clover-T2A-BSR.

    Depositor

    Kazutoshi Takahashi

    Article

    Okubo et al Sci Adv. 2025 Apr 11;11(15):eadq5484. do

    Insert

    SOX2 (SOX2 Human)
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mouse U6 promoter

    Available since

    Sept. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. PX459_NRF2-exon5-1

    Plasmid

    #177781

    Purpose

    sgRNA for CRISPR/Cas9-mediated deletion of human NRF2

    Depositor

    Michael Ristow

    Article

    Thomas et al Nat Commun. 2023 Dec 8;14(1):8142. doi:

    Insert

    NRF2 (NFE2L2 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. PX459_NRF2-exon5-2

    Plasmid

    #177782

    Purpose

    sgRNA for CRISPR/Cas9-mediated deletion of human NRF2

    Depositor

    Michael Ristow

    Article

    Thomas et al Nat Commun. 2023 Dec 8;14(1):8142. doi:

    Insert

    NRF2 (NFE2L2 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSJX067

    Plasmid

    #232328

    Purpose

    Genome editing in Agrobacterium fabrum

    Depositor

    Wei Zhao

    Article

    Sun et al bioRxiv 2024.12.02.626314

    Inserts

    sgRNA
    SpCas9M
    

    Use

    CRISPR

    Tags

    H-arms and sfGFP

    Expression

    Bacterial

    Mutation

    Promoter

    PJ23119 and Pvan

    Available since

    July 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV.Ple155-CI-EGFP-W3SL_BbsI(GGA)

    Plasmid

    #231363

    Purpose

    Ple155-driven EGFP, containing cassette for BbsI-based Golden Gate assembly (e.g. of sgRNA cassette(s)). Can be used as 'no guide' control.

    Depositor

    Viviana Gradinaru

    Article

    Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

    Insert

    EGFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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