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ND4.2-Right DdCBE-G1397-C-wildtype

Plasmid

#179687

Purpose

Expression of base editor in mammalian cells

Depositor

David Liu

Article

Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

Insert

SOD2 MTS_3xHA_ND4.2 Right TALE_2aa_DddA G1397-C )_1xUGI_SOD2 3'UTR

Expression

Mammalian

Available Since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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LbCas12a-D156R

Plasmid

#213744

Purpose

For circular RNA-mediated prime editor using LbCas12a-D156R in HEK293T cells

Depositor

Caixia Gao

Article

Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

Insert

LbCas12a

Use

CRISPR

Tags

BPNLS

Expression

Mammalian

Mutation

D156R

Promoter

CMV

Available Since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pFastBac_human-IL-18

Plasmid

#214338

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

IL18 (IL18 Human)

Tags

His-TEV

Expression

Bacterial

Promoter

T7

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT-ChemoR-ATP

Plasmid

#193820

Purpose

CMV overexpression of ChemR-ATP in mammalian cell lines

Depositor

Julien Hiblot

Article

Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

Insert

ChemoR-ATP

Expression

Mammalian

Promoter

CMV

Available Since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

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p2T-CMV-evoCjCas9-BE4max-BlastR

Plasmid

#194061

Purpose

Mammalian expression of evoCjCas9 CBE4max

Depositor

Gerald Schwank

Article

Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

Insert

evoCjCas9-BE4max

Use

CRISPR

Expression

Mammalian

Available Since

Sept. 21, 2023

Availability

Academic Institutions and Nonprofits only

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tracrRNA(scr)-CJ8421_04975 mRNA

Plasmid

#199718

Purpose

Expresses the Campylobacter mRNA CJ8421_04975 and tracrRNA with scrambled anti-repeat sequencesequence

Depositor

Chase Beisel

Article

Jiao et al Nat Biotechnol. 2023 Jan 5. doi: 10.1038

Insert

CJ8421_04975 mRNA and scrambled tracrRNA

Use

CRISPR

Expression

Bacterial

Available Since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pFRT/TO/FLAG/HA-DEST TNRC6C

Plasmid

#19885

Depositor

Thomas Tuschl

Article

Landthaler et al RNA. 2008 Dec;14(12):2580-96. doi: 10.12

Insert

Trinucleotide repeat containing 6C (TNRC6C Human)

Tags

FLAG/HA

Expression

Mammalian

Available Since

Dec. 31, 2008

Availability

Academic Institutions and Nonprofits only

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pJIT163-AFID-3

Plasmid

#167359

Purpose

For inducing predictable deletions in protoplasts and wheat plants

Depositor

Caixia Gao

Article

Wang et al Nat Biotechnol. 2020 Dec;38(12):1460-146

Insert

A3A-Cas9-UDG-T2A-AP lyase

Expression

Plant

Mutation

WT

Available Since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT-ChemoL-CaM

Plasmid

#193830

Purpose

CMV overexpression of ChemoL-CaM in mammalian cell lines

Depositor

Julien Hiblot

Article

Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

Insert

ChemoL-CaM

Expression

Mammalian

Mutation

EGFP:A206K, E299D; HT7: L271E

Promoter

CMV

Available Since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

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pDonor14-ShoHELIX-KanR (BO4)

Plasmid

#181795

Purpose

pDonor for ShoHELIX containing 14bp of spacing between the I-AniI site and LE/RE using ShCAST flanking sequence and encoding a kanamycin resistance gene as cargo

Depositor

Benjamin Kleinstiver

Article

Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

KanR, R6K origin of replication

Expression

Bacterial

Available Since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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CA-0113 CMVTO-Renin(mouse)-ECL

Plasmid

#231200

Purpose

Membrane-bound mouse renin

Depositor

Xiaojing Gao

Article

Aldrete et al Nat Chem Biol. 2025 Jan 15. doi: 10.1038

Insert

Renin(mouse)-ECL (Ren1 Mouse)

Expression

Mammalian

Available Since

Jan. 28, 2025

Availability

Academic Institutions and Nonprofits only

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mEmerald-MAP4-C-10

Plasmid

#54152

Purpose

Localization: Microtubules, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Article

Planchon et al Nat Methods. 2011 May;8(5):417-23. doi:

Insert

MAP4

Tags

mEmerald

Expression

Mammalian

Mutation

aa 640-1045 of MAP4

Promoter

CMV

Available Since

July 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pD2529-CAG-HA-mouse LRRC33

Plasmid

#180259

Purpose

Mouse full length LRRC33 expression

Depositor

Timothy Springer

Article

Jiang et al Cancer Immunol Res. 2022 Feb 18. pii: 68

Insert

LRRC33 (Nrros Mouse)

Tags

HA

Expression

Mammalian

Available Since

March 17, 2022

Availability

Academic Institutions and Nonprofits only

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p416 25Q GAL

Plasmid

#1185

Depositor

Susan Lindquist

Article

Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

Insert

htt 25Q (HTT Human)

Tags

GFP

Expression

Yeast

Available Since

May 25, 2005

Availability

Academic Institutions and Nonprofits only

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pBMN-FKBP DD(L106P)-UbK0G75C-sfGFP

Plasmid

#172001

Purpose

Expression for LIBRON constructs

Depositor

Thomas Wandless

Article

Miyamae et al Cell Chem Biol. 2020 Dec 17;27(12):1573-

Insert

FKBP DD (L106P)-UbK0G75C

Use

Retroviral

Tags

sfGFP

Mutation

change all lysin residues of ubiquitin to Arg, G7…

Available Since

Aug. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT-ChemoL-NAD

Plasmid

#193827

Purpose

CMV overexpression of ChemoL-NAD in mammalian cell lines

Depositor

Julien Hiblot

Article

Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

Insert

ChemoL-NAD

Expression

Mammalian

Mutation

EGFP:A206K, T225R; HT7: L271E

Promoter

CMV

Available Since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-Cas9-NRRH

Plasmid

#136924

Purpose

Expresses Cas9-NRRH in mammalian cells

Depositor

David Liu

Article

Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

Insert

Cas9-NRRH

Use

CRISPR

Expression

Mammalian

Mutation

see manuscript

Promoter

CMV

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pSLS.491

Plasmid

#184956

Purpose

Test effect of a1/a2 length on editing rpoB using retron recombineering

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Eco1 RT and recombineering ncRNA, rpoB T1534C, a1/a2 length: 12

Expression

Bacterial

Mutation

rpoB donor T1534C

Promoter

T7/lac

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pRN3P_AID-Dam-only

Plasmid

#136065

Purpose

in vitro transcription for mRNA

Depositor

Maria-Elena Torres-Padilla

Article

Borsos et al Nature. 2019 May;569(7758):729-733. doi:

Insert

AID-Dam only

Use

In vitro transcription

Available Since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

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pJJB1561

Plasmid

#218626

Purpose

Express geraniol synthase (GES), Idi1, and Erg20(F96W, N127W) all with ePTS1 tags and cytosolic Erg20_WT_synthetic homodimer

Depositor

John Dueber

Article

Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

Insert

GES

Tags

ePTS1

Expression

Yeast

Available Since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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