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We narrowed to 14,241 results for: Cre

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Showing: 7661 - 7680 of 14241 results

  3. pFN35K SmBiT TK-neo Human collagen type IV alpha 3 chain (COL4A3)

    Plasmid
    #229767
    Purpose
    Human Col4A3 tagged at N-terminal with SmBiT to be used with N-tagged LgBiT hCol4A5 and Flag-tagged hCol4A4 in Nanobit assay system
    Depositor
    Hirofumi Kai
    Article
    Omachi et al Cell Chem Biol. 2018 May 17;25(5):634-64
    Insert
    Human collagen type IV alpha 3 chain (COL4A3) (COL4A3 Human)
    Use
    Luciferase
    Tags
    SmBiT
    Mutation
    See Depositor Comments
    Available Since
    Feb. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    229767-plasmid-map-sequence-id-457423

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  5. pLIB_3xFlag-His-nsp5CS-nsp9 (SARS-CoV-2)

    Plasmid
    #169177
    Purpose
    Baculoviral transfer vector to express SARS-CoV-2 nsp9 with nsp5-cleavable 3xFlag-His6 tag in insect cells
    Depositor
    John Diffley
    Article
    Milligan et al Biochem J. 2021 Jul 16;478(13):2499-2515
    Insert
    3xFlag-His-nsp5CS-nsp9 (ORF1ab SARS-CoV-2)
    Tags
    3xFlag-His6 (nsp5-cleavable)
    Expression
    Insect
    Mutation
    Codon optimised for insect cells (Spodoptera frug…
    Promoter
    Polyhedrin
    Available Since
    July 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    169177-plasmid-map-sequence-id-333223

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  6. pFUSEss-CHIg-mG1_CH3-3

    Plasmid
    #105853
    Purpose
    Expression plasmid coding for hybrid heavy chain of mouse IgG1 antibody specific to antigen B of the ABO blood group system, clone M18. The antibody comprises IgG3-derived CH3.
    Depositor
    Joanna Bereta
    Article
    Klaus et al Front Immunol. 2018 May 23;9:1096. doi:
    Insert
    IgG1 heavy chain, IgG3 heavy chain (Ighg1, Ighg3 Mouse)
    Expression
    Mammalian
    Mutation
    hybrid heavy chain of mouse IgG1 with CH3 derived…
    Promoter
    hEF1-HTLV prom
    Available Since
    April 25, 2018
    Availability
    Academic Institutions and Nonprofits only
    105853-plasmid-map-sequence-id-305783

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  7. pTT5-hSDC1-S15/25A

    Plasmid
    #52364
    Purpose
    expresses human Syndecan-1 serine 15 and 25 mutated to alanine (numbering excludes signal peptide) in HEK293-EBNA1 (293E) suspension culture.
    Depositor
    Gordon Laurie
    Article
    Zhang et al J Biol Chem. 2013 Apr 26;288(17):12090-1
    Insert
    SDC1 (SDC1 Human)
    Expression
    Mammalian
    Mutation
    S15A, S25A and R95Q (according to numbering in Fi…
    Promoter
    CMV
    Available Since
    Dec. 8, 2014
    Availability
    Academic Institutions and Nonprofits only
    52364-plasmid-map-sequence-id-136249

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  8. pTT5-hSDC1-CS1/CS2

    Plasmid
    #52370
    Purpose
    expresses human Syndecan-1 point mutant at S184A and S194A in HEK293-EBNA1 (293E) suspension culture.
    Depositor
    Gordon Laurie
    Article
    Zhang et al J Biol Chem. 2013 Apr 26;288(17):12090-1
    Insert
    SDC1 (SDC1 Human)
    Expression
    Mammalian
    Mutation
    R95Q, S184A and S194A (according to numbering in …
    Promoter
    CMV
    Available Since
    Dec. 8, 2014
    Availability
    Academic Institutions and Nonprofits only

  10. BAP1-C91A (siRNA resistant)

    Plasmid
    #108438
    Purpose
    Stable expression of BAP1-C91A (catalytically inactive mutant) in mammalian cells, gene has an introduced siRNA-resistance against siBAP1.
    Depositor
    Emanuela Felley-Bosco, Rolf Stahel
    Article
    Okonska et al Mol Cancer Ther. 2019 Oct 16. pii: 1535-
    Insert
    BAP1 (BAP1 Human)
    Expression
    Mammalian
    Mutation
    C91A: c.271T>G, c.272G>C; others: c.1641C&g…
    Promoter
    CMV
    Available Since
    Nov. 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    108438-plasmid-map-sequence-id-209210

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  14. pBIG1b_nsp7-His6-nsp8 (SARS-CoV-2)

    Plasmid
    #169180
    Purpose
    Baculoviral transfer vector to express nsp7-His6-nsp8 fusion protein (SARS-CoV-2) in insect cells
    Depositor
    John Diffley
    Article
    Bertolin et al Biochem J. 2021 Jul 16;478(13):2425-2443
    Insert
    nsp7-His6-nsp8 (ORF1ab SARS-CoV-2, Synthetic)
    Tags
    His6 (internal tag linking nsp7 and nsp8): nsp7-H…
    Expression
    Insect
    Mutation
    Codon optimised for insect cells (Spodoptera frug…
    Promoter
    Polyhedrin
    Available Since
    July 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    169180-plasmid-map-sequence-id-333998

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  17. pBIG2abc_nsp12-3xFlag/nsp7-His6-nsp8 (SARS-CoV-2)

    Plasmid
    #169183
    Purpose
    Baculoviral transfer vector to co-express nsp12-3xFlag and nsp7-His6-nsp8 fusion in insect cells
    Depositor
    John Diffley
    Article
    Bertolin et al Biochem J. 2021 Jul 16;478(13):2425-2443
    Insert
    nsp12-3xFlag/nsp7-His6-nsp8 (ORF1ab SARS-CoV-2)
    Tags
    3xFlag (nsp12 C-terminus) and His6 (as internal t…
    Expression
    Insect
    Mutation
    Codon optimised for insect cells (Spodoptera frug…
    Promoter
    Polyhedrin
    Available Since
    July 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    169183-plasmid-map-sequence-id-333983

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  18. pBIG2abc_nsp12-His6-3xFlag/nsp7-Linker-nsp8 (SARS-CoV-2)

    Plasmid
    #169184
    Purpose
    Baculoviral transfer vector to co-express nsp12-His6-3xFlag and nsp7-Linker-nsp8 fusion in insect cells
    Depositor
    John Diffley
    Article
    Bertolin et al Biochem J. 2021 Jul 16;478(13):2425-2443
    Insert
    nsp12-His6-3xFlag/nsp7-Linker-nsp8 (ORF1ab SARS-CoV-2, Synthetic)
    Tags
    His6-3xFlag (nsp12 C-terminus) and nsp7-nsp8 fusi…
    Expression
    Insect
    Mutation
    Codon optimised for insect cells (Spodoptera frug…
    Promoter
    Polyhedrin
    Available Since
    July 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    169184-plasmid-map-sequence-id-333981

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  19. Desmoplakin I affinity mutant (F40-based tension sensor)

    Plasmid
    #119190
    Purpose
    The affinity mutant for the F40-based human desmoplakin I tension sensor incorporates a point mutation that exhibits enhanced keratin association.
    Depositor
    Alex Dunn
    Article
    Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:
    Insert
    human Desmoplakin I(S2849G)-[mTFP1-F40-mEYFP] (internal-1945) (DSP Human)
    Use
    Transposon
    Tags
    mTFP1-F40-mEYFP
    Expression
    Mammalian
    Mutation
    inserted F40-based tension sensor module after aa…
    Promoter
    TCE
    Available Since
    Jan. 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    119190-plasmid-map-sequence-id-232293

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  20. EBL1-bio

    Plasmid
    #47792
    Purpose
    Expresses enzymatically monobiotinylated full-length EBL1 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
    Depositor
    Gavin Wright
    Article
    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.
    Insert
    Codon-optimised EBL1
    Tags
    enzymatic biotinylation sequence and rat Cd4 d3+4
    Expression
    Mammalian
    Mutation
    Exogenous signal peptide of mouse origin, changed…
    Promoter
    CMV
    Available Since
    Feb. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    47792-plasmid-map-sequence-id-68762

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Showing: 7661 - 7680 of 14241 results