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pBbB18k-GFP

Plasmid

#126137

Purpose

Constitutive expression vector for Escherichia coli. BglBrick compatible.

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

GFPuv

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23117

Available Since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

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pBbB14c-GFP

Plasmid

#126129

Purpose

Constitutive expression vector for Escherichia coli. BglBrick compatible.

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

GFPuv

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23119

Available Since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

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pBbB13c-GFP

Plasmid

#126128

Purpose

Constitutive expression vector for Escherichia coli. BglBrick compatible.

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

GFPuv

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23102

Available Since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

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pBbS15a-RFP

Plasmid

#126190

Purpose

Constitutive expression vector for Escherichia coli. BglBrick compatible.

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23100

Available Since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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1xNLS-pMJ915v2-sfGFP

Plasmid

#88919

Purpose

For expression of modified SpyCas9 in e.coli.

Depositor

Jennifer Doudna

Article

Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

Inserts

Cas9
T7

Use

CRISPR

Expression

Bacterial

Available Since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

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EC.RNe.TYB1.a1-400.wt

Plasmid

#17949

Depositor

David McKay

Article

Caruthers et al J Biol Chem. 2006 Sep 15. 281(37):27046-

Insert

Escherichia coli RNase E residues 1-400

Tags

intein-chitin binding domain

Expression

Bacterial

Mutation

residues 1-400

Available Since

May 29, 2008

Availability

Academic Institutions and Nonprofits only
2xNLS-pMJ915v2-sfGFP

Plasmid

#88920

Purpose

For expression of modified SpyCas9 in e.coli.

Depositor

Jennifer Doudna

Article

Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

Inserts

Cas9
T7

Use

CRISPR

Expression

Bacterial

Available Since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

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pNP154

Plasmid

#106360

Purpose

A vector backbone, including mKate, to be used to make constructs to be inserted as a single copy at a site on Chr II.

Depositor

Jonathan Ewbank

Article

Taffoni et al Elife. 2020 Jan 29;9. pii: 45047. doi: 1

Type

Empty backbone

Expression

Worm

Available Since

July 13, 2018

Availability

Academic Institutions and Nonprofits only

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pDESTFLAGHApuro3-Ago Chi-a

Plasmid

#14075

Depositor

Thomas Tuschl

Article

Yuan et al Mol Cell. 2005 Aug 5. 19(3):405-19.

Insert

Ago Chi-a

Tags

FLAG/HA

Expression

Mammalian

Mutation

Ago Chimera-a

Available Since

Feb. 22, 2007

Availability

Academic Institutions and Nonprofits only
pDESTFLAGHApuro3-Ago Chi-b

Plasmid

#14085

Depositor

Thomas Tuschl

Article

Yuan et al Mol Cell. 2005 Aug 5. 19(3):405-19.

Insert

Ago Chi-b

Tags

FLAG/HA

Expression

Mammalian

Mutation

Ago Chimera-b

Available Since

Feb. 22, 2007

Availability

Academic Institutions and Nonprofits only
pDESTFLAGHApuro3-Ago Chi-c

Plasmid

#14086

Depositor

Thomas Tuschl

Article

Yuan et al Mol Cell. 2005 Aug 5. 19(3):405-19.

Insert

Ago Chi-c

Tags

FLAG/HA

Expression

Mammalian

Mutation

Ago Chimera-c

Available Since

Feb. 22, 2007

Availability

Academic Institutions and Nonprofits only
EC.RNe.TYB1.a1-395.wt

Plasmid

#17950

Depositor

David McKay

Article

Caruthers et al J Biol Chem. 2006 Sep 15. 281(37):27046-

Insert

Escherichia coli RNase E residues 1-395

Tags

intein-chitin binding domain

Expression

Bacterial

Mutation

residues 1-395

Available Since

May 29, 2008

Availability

Academic Institutions and Nonprofits only
tdTurboRFP-pBAD

Plasmid

#54857

Purpose

Localization: Protein Expression Vector , Excitation: 553, Emission: 576

Depositor

Dmitriy Chudakov, Michael Davidson, Dorus Gadella

Article

Merzlyak et al Nat Methods. 2007 Jul;4(7):555-7. Epub 2

Type

Empty backbone

Tags

6xHis and tdTurboRFP

Expression

Bacterial

Available Since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pDESTFLAGHApuro3-Ago Chi-d

Plasmid

#14087

Depositor

Thomas Tuschl

Article

Yuan et al Mol Cell. 2005 Aug 5. 19(3):405-19.

Insert

Ago Chi-d

Tags

FLAG/HA

Expression

Mammalian

Mutation

Ago Chimera-d

Available Since

Feb. 22, 2007

Availability

Academic Institutions and Nonprofits only
pJEP206-AAV-1.3CaMKII-MCS-pA.

Plasmid

#78319

Purpose

AAV vector backbone containing a 1.3CaMKIIa promoter followed by a multiple cloning site(MCS) followed by a poly-Adenylation Signal (pA)

Depositor

Jonathan Ploski

Article

Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

Type

Empty backbone

Use

AAV

Promoter

1.3CaMKIIa

Available Since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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pJEP210-AAV-0.4CaMKII P-MCS-pA

Plasmid

#63084

Purpose

AAV2 vector backbone containing a 0.4CaMKIIa promoter followed by a multiple cloning site(MCS) followed by a poly-Adenylation Signal (pA)

Depositor

Jonathan Ploski

Article

Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

Type

Empty backbone

Use

AAV

Promoter

0.4αCaMKII

Available Since

March 16, 2022

Availability

Academic Institutions and Nonprofits only

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pBbE15c-RFP

Plasmid

#126168

Purpose

Constitutive expression vector for Escherichia coli. BglBrick compatible.

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23100

Available Since

April 16, 2021

Availability

Academic Institutions and Nonprofits only

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pBbS9c-RFP

Plasmid

#126184

Purpose

Constitutive expression vector for Escherichia coli. BglBrick compatible.

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23113

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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pBbS20a-RFP

Plasmid

#126192

Purpose

Constitutive expression vector for Escherichia coli. BglBrick compatible.

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

S23200

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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pBbE14k-RFP

Plasmid

#126153

Purpose

Constitutive expression vector for Escherichia coli. BglBrick compatible.

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23119

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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