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SARS-CoV-2 Mpro protease (C145S mutant - catalytically inactive); aliases: 3CLpro, 3C-like

Plasmid

#204769

Purpose

Bacterial expression of Sars Cov 2 MPRO C145S mutant

Depositor

ASAP Discovery, Frank von Delft

Insert

Sars Cov 2 MPRO (ORF1ab Sars Cov 2)

Expression

Bacterial

Mutation

C145S mutant

Available Since

Nov. 30, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pBJ016_MTB_CoChR_KGC_eGFP_ER2

Plasmid

#209277

Purpose

Ubiquitously expresses CoChR in zebrafish via tol2 transgenesis or in vitro transcription of mRNA

Depositor

Adam Cohen

Article

Jia et al Nature. 2023 Oct;622(7981):149-155. doi:

Insert

CoChR_KGC_eGFP_ER2

Use

Zebrafish expression, in vitro transcription

Promoter

actb2

Available Since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pBJ035_MTB_gtACR2_KGC_eGFP_ER2

Plasmid

#209278

Purpose

Ubiquitously expresses gtACR2 in zebrafish via tol2 transgenesis or in vitro transcription of mRNA

Depositor

Adam Cohen

Article

Jia et al Nature. 2023 Oct;622(7981):149-155. doi:

Insert

gtACR2_KGC_eGFP_ER2

Use

Zebrafish expression, in vitro transcription

Promoter

actb2

Available Since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pBJ037_MTB_FR-GECO1c

Plasmid

#209279

Purpose

Ubiquitously expresses FR-GECO1c in zebrafish via tol2 transgenesis or in vitro transcription of mRNA

Depositor

Adam Cohen

Article

Jia et al Nature. 2023 Oct;622(7981):149-155. doi:

Insert

FR-GECO1c

Use

Zebrafish expression, in vitro transcription

Promoter

actb2

Available Since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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TaraACR1-pmCherry-C1

Plasmid

#204960

Purpose

Expression of TaraACR1 in fusion with mCherry in mammalian cells

Depositor

Peter Hegemann

Article

Oppermann et al eLife. 2024 Oct 14;12:RP90100. doi: 10.7

Insert

TaraACR1

Tags

mCherry

Expression

Mammalian

Promoter

CMV (+ enhancer)

Available Since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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TaraACR2-pmCherry-C1

Plasmid

#204961

Purpose

Expression of TaraACR2 in fusion with mCherry in mammalian cells

Depositor

Peter Hegemann

Article

Oppermann et al eLife. 2024 Oct 14;12:RP90100. doi: 10.7

Insert

TaraACR2

Tags

mCherry

Expression

Mammalian

Promoter

CMV (+ enhancer)

Available Since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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TaraACR3-pmCherry-C1

Plasmid

#204962

Purpose

Expression of TaraACR3 in fusion with mCherry in mammalian cells

Depositor

Peter Hegemann

Article

Oppermann et al eLife. 2024 Oct 14;12:RP90100. doi: 10.7

Insert

TaraACR3

Tags

mCherry

Expression

Mammalian

Promoter

CMV (+ enhancer)

Available Since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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S16-ACR1-pcDNA3.1-mScarlet

Plasmid

#204963

Purpose

Expression of S16-ACR1 fused to mScarlet in mammalian cells

Depositor

Peter Hegemann

Article

Oppermann et al eLife. 2024 Oct 14;12:RP90100. doi: 10.7

Insert

S16-ACR1

Tags

mScarlet

Expression

Mammalian

Promoter

CMV (+ enhancer)

Available Since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pATT-Dest-Cre

Plasmid

#202714

Purpose

Cre recombinase staging plasmid to make transposon insertion libraries.

Depositor

Mary Dunlop

Article

Tague et al ACS Synth Biol. 2023 Oct 3. doi: 10.1021

Insert

Cre

Use

Staging plasmid to make transposon insertion libr…

Available Since

Oct. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pPOTv7-blast-moxNG

Plasmid

#190222

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

moxNeonGreen

Use

Unspecified

Promoter

read through

Available Since

Sept. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pGWT35S-TP-UnaG

Plasmid

#203484

Purpose

For particle bombardment. A plastid transit peptide fused UnaG under the control of the cauliflower mosaic virus (CaMV) 35S promoter.

Depositor

Yutaka Kodama

Article

Ishikawa et al Sci Adv. 2023 Jun 9;9(23):eadh4787. doi:

Insert

Plastid transit peptide fused UnaG

Expression

Bacterial and Plant

Available Since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pJMC-3-Reverse

Plasmid

#197803

Purpose

Level 1 Module 3 destination vector (reverse orientation MoClo insert)

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Type

Empty backbone

Use

Unspecified

Available Since

Aug. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pJMC-5-Reverse

Plasmid

#197807

Purpose

Level 1 Module 5 destination vector (reverse orientation MoClo insert)

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Type

Empty backbone

Use

Unspecified

Available Since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pJMC-2T-Reverse

Plasmid

#197800

Purpose

Level 1 Module 2T destination vector (reverse orientation MoClo insert)

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Type

Empty backbone

Use

Unspecified

Available Since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pJMC-7T-Reverse

Plasmid

#197810

Purpose

Level 1 Module 7T destination vector (reverse orientation MoClo insert)

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Type

Empty backbone

Use

Unspecified

Available Since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pTHY_P0019_T2c_BCD_0.1K

Plasmid

#200057

Purpose

Bicistronic RBS 0.1K Part

Depositor

Ross Thyer

Article

Jansen et al ACS Synth Biol. 2023 Jun 16;12(6):1608-1

Insert

BCD 0.1K

Expression

Bacterial

Available Since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pTHY_P0028_T2c_BCD_18K

Plasmid

#200048

Purpose

Bicistronic RBS 18K Part

Depositor

Ross Thyer

Article

Jansen et al ACS Synth Biol. 2023 Jun 16;12(6):1608-1

Insert

BCD 18K

Use

Synthetic Biology

Available Since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pTHY_P0021_T2c_BCD_0.45K

Plasmid

#200055

Purpose

Bicistronic RBS 0.45K Part

Depositor

Ross Thyer

Article

Jansen et al ACS Synth Biol. 2023 Jun 16;12(6):1608-1

Insert

BCD 0.45K

Expression

Bacterial

Available Since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pTHY_P0020_T2c_BCD_0.2K

Plasmid

#200056

Purpose

Bicistronic RBS 0.2K Part

Depositor

Ross Thyer

Article

Jansen et al ACS Synth Biol. 2023 Jun 16;12(6):1608-1

Insert

BCD 0.2K

Expression

Bacterial

Available Since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pTHY_P0026_T2c_BCD_8.5K

Plasmid

#200050

Purpose

Bicistronic RBS 8.5K Part

Depositor

Ross Thyer

Article

Jansen et al ACS Synth Biol. 2023 Jun 16;12(6):1608-1

Insert

BCD 8.5K

Use

Synthetic Biology

Available Since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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