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pLEX307_PDHA2S293D

Plasmid

#115194

Purpose

Lentiviral transduction and expression of PDHA2S293D into any mammalian cell

Depositor

Mohan Babu

Article

Jessulat et al Nucleic Acids Res. 2021 Jul 9. pii: 6318

Insert

PDHA2S293D (PDHA2 Human)

Use

Lentiviral

Mutation

p.S293D

Promoter

EF1alpha

Available Since

July 5, 2022

Availability

Academic Institutions and Nonprofits only

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pLEX307_PDHA2S291D/S293D

Plasmid

#115195

Purpose

Lentiviral transduction and expression of PDHA2S291D/S293D into any mammalian cell

Depositor

Mohan Babu

Article

Jessulat et al Nucleic Acids Res. 2021 Jul 9. pii: 6318

Insert

PDHA2S291D/S293D (PDHA2 Human)

Use

Lentiviral

Mutation

p.S291D/S293D

Promoter

EF1alpha

Available Since

July 5, 2022

Availability

Academic Institutions and Nonprofits only

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LentiGuide-Puro-NCOA7g3 (BB24)

Plasmid

#139459

Purpose

Lentiviral vector with gRNA targeting human NCOA7 short isoform; includes puromycin selectable marker

Depositor

Caroline Goujon

Article

Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

Insert

NCOA7 short isoform-targeting sgRNA inserted; resistance gene: puroR (NCOA7 Synthetic)

Use

Lentiviral

Expression

Mammalian

Promoter

EF-1a / U6

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pKLV-gRNA-Bcan-Ntrk1_4

Plasmid

#136413

Purpose

Lentiviral expression of gRNAs targeting intron 13 of murine Bcan and intron 10 of murine Ntrk1. Also constitutively expresses Puromycin linked to TagBFP.

Depositor

Massimo Squatrito

Article

Oldrini et al Nat Commun. 2018 Apr 13;9(1):1466. doi:

Insert

U6_sgRNA(Bcan)_U6_sgRNA(Ntrk1)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pKLV-gRNA-Myb-Qk_1

Plasmid

#136415

Purpose

Lentiviral expression of gRNAs targeting intron 4 of murine Qk and intron 9 of murine Myb. Also constitutively expresses Puromycin linked to TagBFP.

Depositor

Massimo Squatrito

Article

Oldrini et al Nat Commun. 2018 Apr 13;9(1):1466. doi:

Insert

U6_sgRNA(Myb)_U6_sgRNA(Qk)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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sgRNA BRCA2 S3070F

Plasmid

#139325

Purpose

Plasmid expressing a sgRNA to introduce BRCA2 S3070F using base editing

Depositor

Alberto Ciccia

Article

Billon et al Cell Rep. 2020 Mar 10;30(10):3280-3295.e

Insert

sgRNA to insert BRCA2 S3070F using base editing

Expression

Mammalian

Promoter

U6

Available Since

May 22, 2020

Availability

Academic Institutions and Nonprofits only

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sgRNA BRCA1 S1363L

Plasmid

#139329

Purpose

Plasmid expressing a sgRNA to introduce BRCA1 S1363L using base editing

Depositor

Alberto Ciccia

Article

Billon et al Cell Rep. 2020 Mar 10;30(10):3280-3295.e

Insert

sgRNA to insert BRCA1 S1363L using base editing

Expression

Mammalian

Promoter

U6

Available Since

May 22, 2020

Availability

Academic Institutions and Nonprofits only

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sgRNA BRCA1 E1754K

Plasmid

#139330

Purpose

Plasmid expressing a sgRNA to introduce BRCA1 E1754K using base editing

Depositor

Alberto Ciccia

Article

Billon et al Cell Rep. 2020 Mar 10;30(10):3280-3295.e

Insert

sgRNA to insert BRCA1 E1754K using base editing

Expression

Mammalian

Promoter

U6

Available Since

May 22, 2020

Availability

Academic Institutions and Nonprofits only

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sgRNA FANCD2 Q223Stop

Plasmid

#139332

Purpose

Plasmid expressing a sgRNA to introduce FANCD2 Q223Stop using base editing

Depositor

Alberto Ciccia

Article

Billon et al Cell Rep. 2020 Mar 10;30(10):3280-3295.e

Insert

sgRNA to insert FANCD2 Q223Stop using base editing

Expression

Mammalian

Promoter

U6

Available Since

May 22, 2020

Availability

Academic Institutions and Nonprofits only

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sgRNA BRCA2 R2896C

Plasmid

#139511

Purpose

Plasmid expressing a sgRNA to introduce BRCA2 R2896C using base editing

Depositor

Alberto Ciccia

Article

Billon et al Cell Rep. 2020 Mar 10;30(10):3280-3295.e

Insert

sgRNA to insert BRCA2 E2896C using base editing

Expression

Mammalian

Promoter

U6

Available Since

May 22, 2020

Availability

Academic Institutions and Nonprofits only

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AAVS1 intron 1 gRNA s3

Plasmid

#132395

Purpose

Targets AAVS1 intron 1, gRNA: GGGGCCACTAGGGACAGGAT, MLM3636 backbone

Depositor

Gerold Schmitt-Ulms

Article

Wang et al Bio Protoc. 2020 May 5;10(9):e3615. doi:

Insert

gRNA targeting AAVS1 intron 1 (PPP1R12C Human)

Use

CRISPR

Expression

Mammalian

Promoter

hU6

Available Since

May 6, 2020

Availability

Academic Institutions and Nonprofits only

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lentiCRISPRv2-Snrnp40

Plasmid

#134255

Purpose

Lentivector for Snrnp40 CRISPR knockout

Depositor

Bruce Beutler

Article

Zhang et al Nat Immunol. 2019 Oct;20(10):1322-1334.

Insert

Snrp40 (Snrnp40 Mouse)

Use

CRISPR and Lentiviral

Mutation

Snrnp40 gRNA “GATAACTATGCGACGTTGAA”

Promoter

U6

Available Since

March 20, 2020

Availability

Academic Institutions and Nonprofits only

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human ETV6 gRNA-3

Plasmid

#133403

Purpose

human ETV6 gRNA-3 is a 20-nt gRNA expression plasmid targeting the second ETV6 exon.

Depositor

Linzhao Cheng

Article

Wu et al Stem Cell Res. 2018 May;29:6-14. doi: 10

Insert

ETV6 (ETV6 Human)

Expression

Mammalian

Promoter

U6

Available Since

Oct. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pPRIME-CMV-GFP-shCrh2

Plasmid

#132710

Purpose

Encodes short hairpin RNA (shRNA) #2 that targets the 3’-untranslated region of the rat Crh gene

Depositor

Robert Messing

Article

Pomrenze et al Cell Rep. 2019 Oct 1;29(1):13-21.e4. doi

Insert

shCrh(2) (Crh Rat)

Use

Lentiviral

Promoter

CMV

Available Since

Oct. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-TIRAP-cGASΔN-HA

Plasmid

#130921

Purpose

Expresses human cGASΔN (aa160-522)-HA with an N terminal fusion of the TIRAP N terminus (aa1-85); Puromycin selection marker

Depositor

Jonathan Kagan

Article

Barnett et al Cell. 2019 Mar 7;176(6):1432-1446.e11. d

Insert

TIRAP-ΔNcGAS

Tags

HA

Expression

Mammalian

Mutation

cGAS N terminus (aa1-159) replaced with the N ter…

Promoter

CMV

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-Fyn-cGASΔN-HA

Plasmid

#130922

Purpose

Expresses human cGASΔN (aa160-522)-HA with an N terminal fusion of the Fyn dual acylation motif (aa1-16); Puromycin selection marker

Depositor

Jonathan Kagan

Article

Barnett et al Cell. 2019 Mar 7;176(6):1432-1446.e11. d

Insert

Fyn-ΔNcGAS

Tags

HA

Expression

Mammalian

Mutation

cGAS N terminus (aa1-159) replaced with the dual …

Promoter

CMV

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pFB-U6-Chrna6-gRNA-hSyn-mCherry-WPRE-SV40pA

Plasmid

#128341

Purpose

pAAV encoding gRNA sequence for loss-of-function indels

Depositor

Ryan Drenan

Article

Peng et al Eur J Neurosci. 2018 May 19. doi: 10.111

Insert

Chrna6 (Chrna6 Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

July 19, 2019

Availability

Academic Institutions and Nonprofits only

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289aa ELL2

Plasmid

#127266

Purpose

For in vitro translation of shorter human ELL2 from Met1. Also contains Met133I, M1381I, M186I mutations.

Depositor

Christine Milcarek

Article

Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

Insert

NH2-ELL2 (Met133ILeu, M1381ILeu, M186ILeu) (ELL2 Human)

Use

In vitro translation

Mutation

Three Mets (133, 138, 186) to Ileu. Contains 289…

Promoter

T7

Available Since

July 17, 2019

Availability

Academic Institutions and Nonprofits only

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HA tagged Xba ELL2 Met133, 138, 186 to Ileu

Plasmid

#127269

Purpose

For in vitro translation of ELL2 with with internal HA tag and Met133, 138, 186 Ileu

Depositor

Christine Milcarek

Article

Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

Insert

ELL2 Met133, 138, 186 to Ileu (ELL2 Human)

Use

In vitro translation

Mutation

Met133, 138, 186 to Ileu HA tag after M186

Promoter

T7

Available Since

July 15, 2019

Availability

Academic Institutions and Nonprofits only

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HA tagged Sph ELL2 Met133, 138, 186 to Ileu

Plasmid

#127271

Purpose

For in vitro translation of human ELL2 with Met133, 138, 186 to Ileu with HA tag after 8th Met

Depositor

Christine Milcarek

Article

Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

Insert

ELL2 Met133, 138, 186 to Ileu (ELL2 Human)

Use

In vitro translation

Mutation

Met133, 138, 186 to Ileu with HA tag after 8th Met

Promoter

T7

Available Since

July 15, 2019

Availability

Academic Institutions and Nonprofits only

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