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pGST-DHFR Mm

Plasmid

#83073

Purpose

Expresses the full length DHFR Mm in E.coli

Depositor

Gali Prag

Publication

Levin-Kravets et al Nat Methods. 2016 Nov;13(11):945-952. do

Insert

2x DHFR (Dhfr Mouse)

Tags

GST

Expression

Bacterial

Available since

Nov. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pCM5.50

Plasmid

#19383

Depositor

Geraldine Seydoux

Publication

Merritt et al Curr Biol. 2008 Oct 14;18(19):1476-82. d

Insert

spn-4 3'utr in pDONRP2RP3 (spn-4 Nematode)

Expression

Worm

Available since

Oct. 1, 2008

Availability

Academic Institutions and Nonprofits only

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VIPR1

Plasmid

#51865

Purpose

Expresses full-length Vasoactive intestinal polypeptide receptor 1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

Depositor

Gavin Wright

Publication

Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

Insert

VIPR1 (VIPR1 Human)

Tags

His tag, enzymatic biotinylation sequence, and ra…

Expression

Mammalian

Promoter

CMV

Available since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pFA6a-kanMX6-P3nmt1

Plasmid

#39280

Depositor

Jurg Bahler, John Pringle

Publication

Bahler et al Yeast. 1998 Jul;14(10):943-51.

Insert

nmt1 promoter (nmt1 Fission Yeast)

Use

Yeast genomic targeting

Tags

A. gossypii translation elongation factor 1a gene…

Mutation

-1163 to +6 of nmt1 locus

Available since

Aug. 14, 2012

Availability

Academic Institutions and Nonprofits only

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pMIR-Report-Luc-KLF4-K5S

Plasmid

#34598

Depositor

Michael Ruppert

Publication

Lin et al Mol Cell Biol. 2011 Jun;31(12):2513-27.

Insert

KLF4 (KLF4 Human)

Use

Luciferase

Expression

Mammalian

Mutation

Fragment 1781-2086 of KLF4

Promoter

CMV

Available since

Feb. 2, 2012

Availability

Academic Institutions and Nonprofits only
ATF4 18: RLuc.5’ATF4.GFP

Plasmid

#21864

Depositor

David Ron

Publication

Lu et al J Cell Biol. 2004 Oct 11. 167(1):27-33.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ATF4 (Atf4 Mouse)

Tags

EGFP

Expression

Mammalian

Available since

Oct. 1, 2009

Availability

Academic Institutions and Nonprofits only
pCMV5 Flag Jnk1a1(APY)

Plasmid

#15742

Depositor

Roger Davis

Publication

Derijard et al Cell. 1994 Mar 25. 76(6):1025-37.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Jnk1a1(TPF) (MAPK8 Human)

Tags

Flag

Expression

Mammalian

Mutation

Thr-190Ala

Available since

March 6, 2008

Availability

Academic Institutions and Nonprofits only
pBR_attB(bxb)_ccdB_lox_v2

Plasmid

#198040

Purpose

Bxb1-specific donor plasmid for the cloning of multiple inserts by Golden Gate assembly (BpiI)

Depositor

Richard Davis

Publication

Blanch-Asensio et al Nat Protoc. 2024 Aug 23. doi: 10.1038/s4

Type

Empty backbone

Use

Cre/Lox and Synthetic Biology

Expression

Mammalian

Available since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

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pMSCVII-BFP

Plasmid

#162755

Purpose

Retroviral (MSCV) expression of mTagBFP2 fluorescent protein

Depositor

Maki Nakayama

Publication

Landry et al Bio Protoc. 2021 Jan 20;11(2):e3883. doi

Insert

mTagBFP2

Use

Retroviral

Expression

Mammalian

Available since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pC239

Plasmid

#65953

Purpose

N2 inverter plasmid for two-strain system

Depositor

Matthew Bennett

Publication

Chen et al Science. 2015 Aug 28;349(6251):986-9. do

Inserts

AiiA
LacI

Tags

LAA tagged

Expression

Bacterial

Promoter

pCin and pCin*

Available since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

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Frb-NLS-mCherry

Plasmid

#217613

Purpose

part B of SPARK-OFF

Depositor

Xiaokun Shu

Publication

Chung et al Proc Natl Acad Sci U S A. 2024 Feb 13;12

Insert

Frb-NLS-mCherry

Use

Lentiviral

Expression

Mammalian

Available since

June 27, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-CCreV

Plasmid

#140132

Purpose

can be used to generate AAV virus that will express fusion protein of split Cre (C-terminal) and fungal photoreceptor Vivid (VVD) monomer

Depositor

Hongkui Zeng

Publication

Yao et al Nat Methods. 2020 Mar 23. pii: 10.1038/s

Insert

CCreV

Use

AAV

Promoter

EF1a

Available since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-NCreV

Plasmid

#140131

Purpose

can be used to generate AAV virus that will express fusion protein of split Cre (N-terminal) and fungal photoreceptor Vivid (VVD) monomer

Depositor

Hongkui Zeng

Publication

Yao et al Nat Methods. 2020 Mar 23. pii: 10.1038/s

Insert

NCreV

Use

AAV

Promoter

EF1a

Available since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

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GNb-mCherry

Plasmid

#128769

Purpose

Mammalian expression

Depositor

Colin Taylor

Publication

Prole et al BMC Biol. 2019 May 23;17(1):41. doi: 10.

Insert

GNb-mCherry

Expression

Mammalian

Available since

Feb. 8, 2020

Availability

Academic Institutions and Nonprofits only

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pJUMP26-1A(sfGFP)

Plasmid

#126973

Purpose

Level 1 vector. sfGFP reporter. Origin p15A (medium copy number)

Depositor

Chris French

Publication

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

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pJUMP45-2A(sfGFP)

Plasmid

#126989

Purpose

Level 2 vector. sfGFP reporter. Origin RSF1010 (Broad-host-range)

Depositor

Chris French

Publication

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pJUMP25-1A(sfGFP)

Plasmid

#126972

Purpose

Level 1 vector. sfGFP reporter. Origin RSF1010 (Broad-host-range)

Depositor

Chris French

Publication

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pCLX-M38s/TOP-dGFP

Plasmid

#114295

Purpose

Expression of destabilized GFP under the control of the M38-TOP canonical Wnt signaling responsive promoter

Depositor

Patrick Salmon

Publication

Boitard et al Cell Rep. 2015 Mar 3;10(8):1349-61. doi:

Insert

dGFP

Use

Lentiviral

Mutation

destabilized GFP

Available since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pFF838

Plasmid

#61457

Purpose

aTc-inducible SCRIBE_lacZ(ON)

Depositor

Timothy Lu

Publication

Farzadfard et al Science. 2014 Nov 14;346(6211):1256272.

Insert

SCRIBE_lacZ(ON)

Use

Synthetic Biology

Promoter

pTetO

Available since

Feb. 20, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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R5 PV

Plasmid

#51734

Purpose

Dicistronic vector with PV IRES

Depositor

Vincent Mauro

Publication

Burman et al Nucleic Acids Res. 2012 Sep;40(16):8085-

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

PV IRES

Promoter

SV40

Available since

July 14, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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