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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 37,317 results for: kan

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Showing: 7741 - 7760 of 37317 results

  1. pfMDH_WT

    Plasmid
    #67721
    Purpose
    Expresses WT mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli
    Depositor
    Justin Siegel
    Publication
    Lucas et al Protein Sci. 2015 Jun;24(6):936-45. doi:
    Insert
    Mannitol 2-Dehydrogenase from Pseudomonas fluorescens
    Tags
    6x-His tag
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Sept. 10, 2015
    Availability
    Academic Institutions and Nonprofits only

  2. pSMART-Mev1

    Plasmid
    #65815
    Purpose
    NADPH Dependent Mevalonic Acid Production Construct, Low Phosphate Dependent E. coli Expression
    Depositor
    Michael Lynch
    Inserts
    mvaE: bifunctional acetoacetyl-CoA thiolase, and NADPH dependent HMG-CoA reductase
    mvaS: hydroxymethylglutaryl-CoA synthase
    Expression
    Bacterial
    Promoter
    insulated mipAp from E. coli and insulated waaHp…
    Available since
    Sept. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    65815-plasmid-map-sequence-id-111947

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  8. alpha actinin delta ABD-GFP

    Plasmid
    #66935
    Purpose
    Mammalian expression of alpha actinin with actin binding domain (amino acids 30–253) deleted
    Depositor
    Pere Roca-Cusachs, Michael Sheetz
    Publication
    Roca-Cusachs et al Proc Natl Acad Sci U S A. 2013 Apr 9;110
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Alpha-actinin 1 (ACTN1 Human)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    actin binding domain (amino acids 30–253) is dele…
    Promoter
    CMV
    Available since
    June 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by

  11. 4RCI

    Plasmid
    #64653
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    YTHDF1:JMC02A-E04:C231829 (YTHDF1 Human)
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    See comments
    Promoter
    T7
    Available since
    April 29, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by

  12. tdKatushka2-CENPB-N-22

    Plasmid
    #56033
    Purpose
    Localization: Nucleus/Centromeres, Excitation: 586, Emission: 632
    Depositor
    Michael Davidson
    Publication
    Shcherbo et al Biochem J. 2009 Mar 15;418(3):567-74. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CENPB (CENPB Human)
    Tags
    tdKatushka2
    Expression
    Mammalian
    Mutation
    aa 1-169 of CENPB (DNA Binding Domain)
    Promoter
    CMV
    Available since
    April 28, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  13. mT-Sapphire-H2B-6

    Plasmid
    #56404
    Purpose
    Localization: Nucleus/Histones, Excitation: 399, Emission: 511
    Depositor
    Michael Davidson
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    H2B (H2BC11 Human)
    Tags
    mT
    Expression
    Mammalian
    Mutation
    D26G and V119I in H2B
    Promoter
    CMV
    Available since
    April 28, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  14. mIFP-Calnexin-N-14

    Plasmid
    #56214
    Purpose
    Localization: Endoplasmic Reticulum, Excitation: 683, Emission: 704
    Depositor
    Michael Davidson
    Publication
    Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Calnexin (CANX Human)
    Tags
    mIFP
    Expression
    Mammalian
    Mutation
    I474N in Calnexin
    Promoter
    CMV
    Available since
    April 28, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by

  15. mIFP-Lysosomes-20

    Plasmid
    #56228
    Purpose
    Localization: Lysosome Membrane, Excitation: 683, Emission: 704
    Depositor
    Michael Davidson
    Publication
    Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    LAMP1
    Tags
    mIFP
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    April 28, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  16. Tom20-mChF-AIP(TA)

    Plasmid
    #61513
    Purpose
    Expression of human FK506 binding protein 1A (FKBP1A) tagged with mCherry, Tom20, and AIP with TA mutation
    Depositor
    Takanari Inoue
    Publication
    Miyamoto et al Cell Rep. 2015 Apr 28;11(4):657-70. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    FK506 binding protein 1A (FKBP1A Human)
    Tags
    AIP with TA mutation (see comments), Tom20, and m…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    April 17, 2015
    Availability
    Academic Institutions and Nonprofits only

  17. mChF-AIP

    Plasmid
    #61527
    Purpose
    Expression of human FK506 binding protein 1A (FKBP1A) tagged with mCherry and AIP
    Depositor
    Takanari Inoue
    Publication
    Miyamoto et al Cell Rep. 2015 Apr 28;11(4):657-70. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    FK506 binding protein 1A (FKBP1A Human)
    Tags
    AIP and mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    April 17, 2015
    Availability
    Academic Institutions and Nonprofits only

  20. mRuby2-Farnesyl-5

    Plasmid
    #55896
    Purpose
    Localization: Membrane, Excitation: 559, Emission: 600
    Depositor
    Michael Davidson
    Publication
    Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Farnesyl (HRAS Human)
    Tags
    mRuby2
    Expression
    Mammalian
    Mutation
    encodes final 20 aa of NM_001130442.1
    Promoter
    CMV
    Available since
    April 1, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    55896-plasmid-map-sequence-id-169601

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Showing: 7741 - 7760 of 37317 results