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R0063 (pLuxR-pR)_EB

Plasmid

#66013

Purpose

MoClo Basic Part: Controllable promoter - pLuxR(pR) - luxR regulated (right promoter, weak constitutive down regulated by LuxR, C0062 in combination with homoserine lactone (HSL)) [E:R0063:B]

Depositor

Douglas Densmore

Article

Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

Insert

Controllable promoter

Use

Synthetic Biology

Available Since

Jan. 21, 2016

Availability

Academic Institutions and Nonprofits only

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R0063 (pLuxR-pR)_FB

Plasmid

#66014

Purpose

MoClo Basic Part: Controllable promoter - pLuxR(pR) - luxR regulated (right promoter, weak constitutive down regulated by LuxR, C0062 in combination with homoserine lactone (HSL)) [F:R0063:B]

Depositor

Douglas Densmore

Article

Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

Insert

Controllable promoter

Use

Synthetic Biology

Available Since

Jan. 21, 2016

Availability

Academic Institutions and Nonprofits only

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R0063 (pLuxR-pR)_GB

Plasmid

#66015

Purpose

MoClo Basic Part: Controllable promoter - pLuxR(pR) - luxR regulated (right promoter, weak constitutive down regulated by LuxR, C0062 in combination with homoserine lactone (HSL)) [G:R0063:B]

Depositor

Douglas Densmore

Article

Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

Insert

Controllable promoter

Use

Synthetic Biology

Available Since

Jan. 21, 2016

Availability

Academic Institutions and Nonprofits only

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C0012m (lacI)_CD

Plasmid

#66026

Purpose

MoClo Basic Part: CDS - Controller protein, lacI repressor (in concert with CAP, represses pLacI, R0010). Modified to fix illegal site. [C:C0012m:D]

Depositor

Douglas Densmore

Article

Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

Insert

Transcription factor

Use

Synthetic Biology

Available Since

Jan. 21, 2016

Availability

Academic Institutions and Nonprofits only

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C0062 (luxR)_CD

Plasmid

#66028

Purpose

MoClo Basic Part: CDS - Controller protein, luxR repressor/activator (in concert with HSL, represses pLuxR(pR) R0063. Also up-regulates pLuxR(pL) R0062) [C:C0062:D]

Depositor

Douglas Densmore

Article

Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

Insert

Transcription factor

Use

Synthetic Biology

Available Since

Jan. 21, 2016

Availability

Academic Institutions and Nonprofits only

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R0040 (pTet)_AB

Plasmid

#66008

Purpose

MoClo Basic Part: Controllable promoter - pTet - tetR regulated (strong constitutive promoter repressed by tetR, C0040, which can itself be inhibited by tetracycline or aTc) [A:R0040:B]

Depositor

Douglas Densmore

Article

Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

Insert

Controllable promoter

Use

Synthetic Biology

Available Since

Jan. 21, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
R0040 (pTet)_EB

Plasmid

#66009

Purpose

MoClo Basic Part: Controllable promoter - pTet - tetR regulated (strong constitutive promoter repressed by tetR, C0040, which can itself be inhibited by tetracycline or aTc) [E:R0040:B]

Depositor

Douglas Densmore

Article

Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

Insert

Controllable promoter

Use

Synthetic Biology

Available Since

Jan. 21, 2016

Availability

Academic Institutions and Nonprofits only

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R0040 (pTet)_FB

Plasmid

#66010

Purpose

MoClo Basic Part: Controllable promoter - pTet - tetR regulated (strong constitutive promoter repressed by tetR, C0040, which can itself be inhibited by tetracycline or aTc) [F:R0040:B]

Depositor

Douglas Densmore

Article

Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

Insert

Controllable promoter

Use

Synthetic Biology

Available Since

Jan. 21, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
R0040 (pTet)_GB

Plasmid

#66011

Purpose

MoClo Basic Part: Controllable promoter - pTet - tetR regulated (strong constitutive promoter repressed by tetR, C0040, which can itself be inhibited by tetracycline or aTc) [G:R0040:B]

Depositor

Douglas Densmore

Article

Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

Insert

Controllable promoter

Use

Synthetic Biology

Available Since

Jan. 21, 2016

Availability

Academic Institutions and Nonprofits only

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Hoxd13-3'UTR

Plasmid

#31525

Depositor

Chang-Zheng Chen

Article

Arnold et al Genome Res. 2011 May . 21(5):798-810.

Insert

Hoxd13-3'UTR (Hoxd13 Mouse)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available Since

Oct. 4, 2011

Availability

Academic Institutions and Nonprofits only
pPKm-300

Plasmid

#104626

Purpose

Expresses pSIN - EF-1alpha - MTS - tFd, vector encoding mitochondrial-tagged Thermosynechococcus elongates Ferredoxin (Fd), under EF-1alpha promoter

Depositor

Todd Coleman

Article

Kyriakakis et al ACS Synth Biol. 2018 Feb 16;7(2):706-717

Insert

tFd

Use

Lentiviral

Expression

Mammalian

Promoter

EF-1alpha

Availability

Academic Institutions and Nonprofits only

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AAV-PR rep/cap

Plasmid

#197565

Purpose

encodes AAV-PR capsid that transduces pericytes and smooth muscle cells in mice after systemic delivery

Depositor

Casey Maguire

Article

Ramirez et al Hum Gene Ther. 2023 Jun 27. doi: 10.1089

Inserts

AAV Rep genes
AAV9 VP1 Cap gene with PR insert

Use

AAV

Mutation

contains 7mer insert PRPPSTH between amino acids …

Available Since

June 12, 2023

Availability

Academic Institutions and Nonprofits only

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pSLQ9715: pHR-PGK-SV40_NLS-dCasMINI-V4-NFZ-c-Myc_NLS-mCherry-WPRE

Plasmid

#203221

Purpose

Expression of dCasMINI-V4-NFZ-mCherry in mammalian cells

Depositor

Stanley Qi

Article

Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

Insert

dCasMINI-V4-NFZ-mCherry

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

PGK

Available Since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

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pMB1_MmPylRS_MmtRNA-Pyl-opt(UGA)

Plasmid

#174515

Purpose

Recoded single aaRS/tRNA plasmid with MmPylRS and MmtRNA-Pyl-opt with the ASL from the serT gene (UGA anticodon)

Depositor

Jason W Chin

Article

Robertson et al Science. 2021 Jun 4;372(6546):1057-1062.

Inserts

Mm PylS
MmtRNA-Pyl-opt(UGA)

Expression

Bacterial

Mutation

ASL from the serT gene (UGA anticodon) and recoded

Promoter

GlnS and lpp

Available Since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-15nmEML-mRFP

Plasmid

#231177

Purpose

Stabilizes the ER-mitochondrial distance at 15 nm; tagged with mRFP

Depositor

Dmitry Lim

Article

Dematteis et al Commun Biol. 2024 Oct 10;7(1):1294. doi:

Insert

15nmEML-mRFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-ER-mRFP

Plasmid

#231176

Purpose

Expresses control plasmid for ER-mitochondrial linkers, tagged with mRFP, in mammalian cells

Depositor

Dmitry Lim

Article

Dematteis et al Commun Biol. 2024 Oct 10;7(1):1294. doi:

Insert

ER-mRFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-30nmEML-mRFP

Plasmid

#231179

Purpose

Stabilizes the ER-mitochondrial distance at 30 nm; tagged with mRFP

Depositor

Dmitry Lim

Article

Dematteis et al Commun Biol. 2024 Oct 10;7(1):1294. doi:

Insert

30nmEML-mRFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-20nmEML-mRFP

Plasmid

#231178

Purpose

Stabilizes the ER-mitochondrial distance at 20 nm; tagged with mRFP

Depositor

Dmitry Lim

Article

Dematteis et al Commun Biol. 2024 Oct 10;7(1):1294. doi:

Insert

20nmEML-mRFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.2/V5-DEST RBM14-V5

Plasmid

#69823

Purpose

Expresses RBM14-V5 in mammalian cells

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

RNA-binding protein 14 (RBM14 Human)

Use

Retroviral

Tags

v5

Expression

Mammalian

Promoter

cmv

Available Since

April 25, 2016

Availability

Academic Institutions and Nonprofits only

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pLV-CMV-dnMCAK

Plasmid

#205993

Purpose

Over-expression of GFP-dnMCAK

Depositor

Floris Foijer

Article

Hong et al Nature. 2022 Jul;607(7918):366-373. doi:

Insert

dnMCAK

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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