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pQE30-HaAEP1

Plasmid

#159530

Purpose

Expresses sunflower (Helianthus annuus) asparaginyl endopeptidase 1 in E. coli

Depositor

Joshua Mylne

Article

Bernath-Levin et al Chem Biol. 2015 May 21;22(5):571-82. doi

Insert

Helianthus annuus asparaginyl endopeptidase 1 (LOC110922574 Synthetic)

Tags

Six-Histidine tag

Expression

Bacterial

Promoter

T5

Available Since

Oct. 16, 2020

Availability

Academic Institutions and Nonprofits only

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RRAS2

Plasmid

#55660

Purpose

expression of mouse RRAS2

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

RRAS2 (Rras2 Mouse)

Expression

Bacterial

Promoter

lac

Available Since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only
pCMV-3×FLAG-SLC35A1

Plasmid

#186282

Purpose

transient expression of N-terminal 3×FLAG tagged SLC35A1 isoform a in mammalian cells

Depositor

Somshuvra Mukhopadhyay

Article

Li et al J Biol Chem. 2021 Sep;297(3):101069. doi

Insert

SLC35A1 (SLC35A1 Human)

Tags

3×FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 5, 2022

Availability

Academic Institutions and Nonprofits only

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pJC45BS104 Y819W

Plasmid

#1307

Depositor

Susan Lindquist

Article

Cashikar et al Mol Cell 2002 Apr;9(4):751-60.

Insert

Hsp104 Y819W (HSP104 Budding Yeast)

Tags

10X His tag

Expression

Bacterial

Mutation

Changed Tyr 819 to Trp

Available Since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only

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p3E-ATG18

Plasmid

#109556

Purpose

Multisite gateway vector for 3' tagging with yeast ATG18 (PLEKHA3). Binds PtdIns(3,5P)2

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

yeast ATG18 (PLEKHA3)

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pAG186 NID1 3'UTR

Plasmid

#12016

Depositor

David Bartel

Article

Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

Insert

N19 3'UTR (NID1 Human)

Use

Luciferase

Expression

Mammalian

Available Since

June 2, 2006

Availability

Academic Institutions and Nonprofits only

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pRS426-GFP-Num1-PH

Plasmid

#71374

Purpose

For analysis of PH domain localization in yeast

Depositor

Mark Lemmon

Article

Yu et al Mol Cell. 2004 Mar 12;13(5):677-88.

Insert

Num1-PH

Tags

EGFP

Expression

Yeast

Mutation

Pleckstrin homology (PH) domain; aa2563-2692

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only
pHH0103_ARFGEF2_Sec7

Plasmid

#109924

Purpose

Protein expression and purification of ARFGEF2_Sec7

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

ARFGEF2_Sec7

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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TOPO Venus

Plasmid

#68493

Purpose

TOPO construct expressing Venus for generation of GMAP-compatible gene

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Insert

split Venus

Use

Gmap

Expression

Bacterial

Promoter

Plac

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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AL12R-EF-Tu

Plasmid

#161013

Purpose

PP7-tagged reporter driven by the Arabidopsis EF-Tu promoter

Depositor

Krishna Niyogi

Article

Alamos et al Nat Plants. 2021 Aug;7(8):1037-1049. doi

Insert

PP7 and H2B-mScarlet

Expression

Plant

Available Since

Dec. 11, 2020

Availability

Academic Institutions and Nonprofits only

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CMVTCRb_candidate2

Plasmid

#164998

Purpose

Expression of candidate TCR #2 TCRbeta cDNA

Depositor

Matthew Meyerson

Article

Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

Insert

TRB (TRB Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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CMVTCRa_candidate2

Plasmid

#164997

Purpose

Expression of candidate TCR #2 TCRalpha cDNA

Depositor

Matthew Meyerson

Article

Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

Insert

TRAV (TRA Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-mNoxa1

Plasmid

#58339

Purpose

expresses mouse Noxa1 in mammalian cells

Depositor

Botond Banfi, Karl-Heinz Krause

Article

Banfi et al J Biol Chem. 2003 Feb 7;278(6):3510-3. E

Insert

NADPH oxidase activator 1 (Noxa1 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 25, 2014

Availability

Academic Institutions and Nonprofits only

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RhoC

Plasmid

#55702

Purpose

expression of mouse RhoC

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

RhoC (Rhoc Mouse)

Expression

Bacterial

Promoter

lac

Available Since

Sept. 30, 2014

Availability

Academic Institutions and Nonprofits only
pGL2B -55/+64

Plasmid

#24948

Depositor

Stephen Smale

Article

Brightbill et al J Immunol. 2000 Feb 15. 164(4):1940-51.

Insert

IL-10 promoter (Il10 Mouse)

Use

Luciferase

Tags

Luciferase

Available Since

June 4, 2010

Availability

Academic Institutions and Nonprofits only
pME-myristo

Plasmid

#109541

Purpose

Multisite gateway vector for 5' tagging with the 5' myristoylation sequence from ARF5

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

5' myristoylation sequence from ARF5

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-cNanog-pgk-hph

Plasmid

#74907

Purpose

Mammalian expression of cNanog

Depositor

Jose Silva

Article

Theunissen et al Development. 2011 Nov;138(22):4853-65. d

Insert

Nanog

Expression

Mammalian

Promoter

CAG

Available Since

May 17, 2016

Availability

Academic Institutions and Nonprofits only

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peSpdCas9-J23109

Plasmid

#113153

Purpose

Plasmid constitutively expressing eSpdCas9 protein

Depositor

Chong Zhang

Article

Guo et al Nucleic Acids Res. 2018 Aug 21;46(14):70

Insert

eSpdCas9

Expression

Bacterial

Mutation

D10A & K810A & H840A & K1003A & R…

Available Since

Dec. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pCJH031_proD_LbCas12a-gRNA-GFP_CAM

Plasmid

#183075

Purpose

Plasmid expressing Cas12a guide RNA targeting GFP (1) for in vivo interference assay in bacteria.

Depositor

Jennifer Doudna

Article

Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

Insert

LbCas12a guide RNA targeting GFP

Use

CRISPR

Expression

Bacterial

Available Since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO.1 Puro shRNA PTEN KD2

Plasmid

#162009

Purpose

PTEN shRNA

Depositor

David Bryant

Article

Roman-Fernandez et al Nat Commun. 2018 Nov 28;9(1):5041. doi:

Insert

PTEN (PTEN Canis Lupus)

Use

Lentiviral

Available Since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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