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pLJM1-B-GRAM-H-NeoR

Plasmid

#211709

Purpose

Lentiviral expression of a dimerization-dependent fluorescent protein (ddFP) subunit (B) fused with the GRAM domain of GRAMD1b carrying a G187L mutation (B-GRAM-H)

Depositor

Yasunori Saheki

Article

Koh et al Nat Commun. 2023 Oct 25;14(1):6773. doi:

Insert

B-tagged GRAM domain of GRAMD1b/Aster-B (GRAMD1B Human)

Use

Lentiviral

Tags

ddFP-B3

Expression

Mammalian

Mutation

Changed Glycine 187 to Leucine

Promoter

CMV

Available Since

Jan. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pBFC0687

Plasmid

#186692

Purpose

ShDART under control of Lac promoter, original configuration (pre-gRNA terminator and J23119 promoter), Sh_2xBsaI_NT gRNA, GmR+sfGFP cargo

Depositor

Jennifer Doudna

Article

Rubin et al Nat Microbiol. 2022 Jan;7(1):34-47. doi:

Inserts

tnsB
tnsC
tniQ
Cas12k
Sh_2xBsaI_NT (Guide Stuffer) gRNA
Tn7 transposon
GmR+sfGFP ShDART Cargo

Expression

Bacterial

Promoter

PLac

Available Since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pDendra2-5'UTR(GAPDH)-Dendra2-3'UTR(GAPDH)

Plasmid

#182368

Purpose

Dendra2-based translation reporter

Depositor

Chunghun Lim

Article

Lee et al BMC Biol. 2022 Jan 7;20(1):12. doi: 10.1

Insert

glyceraldehyde-3-phosphate dehydrogenase (Gapdh Mouse)

Tags

Pal-Dendra2

Expression

Mammalian

Promoter

CMV promoter

Available Since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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PB-mCar-EGFP-WPRE-BGH-pA-UBC-Bla

Plasmid

#174620

Purpose

PiggyBac plasmid expressing EGFP under the control of a cone arrestin promoter

Depositor

Volker Busskamp

Article

Gasparini et al J Clin Invest. 2022 Apr 28. pii: 154619.

Insert

enhanced green fluorescent protein

Use

Piggybac

Expression

Mammalian

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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EC2_10_dCas9_VPR_HC_sgRNA

Plasmid

#163712

Purpose

Yeast high copy plasmid with dCas9, sgRNA expression cassette and VPR activation domains

Depositor

Yvonne Nygård

Article

Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

Inserts

dCas9
VPR

Expression

Yeast

Mutation

Point mutations D10A and H840A

Promoter

ScTEF1

Available Since

Nov. 1, 2021

Availability

Academic Institutions and Nonprofits only

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EC2_4_dCas9_VPR_sgRNA

Plasmid

#163709

Purpose

Yeast low copy plasmid with dCas9, sgRNA expression cassette and VPR activation domains

Depositor

Yvonne Nygård

Article

Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

Inserts

dCas9
VPR

Expression

Yeast

Mutation

Point mutations D10A and H840A

Promoter

ScTEF1

Available Since

Nov. 1, 2021

Availability

Academic Institutions and Nonprofits only

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ppBAalbNixE1E2 OpIE2-GFP

Plasmid

#173505

Purpose

transgenesis vector for Aedes albopictus ectopic expression of the Nix gene

Depositor

Eric Marois

Article

Lutrat et al Commun Biol. 2022 Mar 7;5(1):210. doi: 1

Inserts

Nix masculinisation gene
EGFP

Expression

Insect

Mutation

Nix isoforms with exons 1, 2 separated by intron 1

Promoter

OpIE2 and own

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pM124: pAAV-EFS-CasRx-VEGFA presgRNA

Plasmid

#166872

Purpose

AAV vector for expressing CasRx and human VEGFA targeting presgRNA for RNA-editing

Depositor

Guei-Sheung Liu

Article

Chuang et al Front Cell Dev Biol. 2021 Jun 11;9:66787

Inserts

U6-VEGFA presgRNA
RfxCas13d

Use

AAV and CRISPR

Tags

HA and NLS

Expression

Mammalian

Promoter

EFS and U6

Available Since

July 1, 2021

Availability

Academic Institutions and Nonprofits only

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pMS1: pHelper(AvCAST)_AvPSP1

Plasmid

#168133

Purpose

Inducible expression of AvCAST proteins with crRNA targeting AvPSP1.

Depositor

Feng Zhang

Article

Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

Inserts

AvCAST minimal CRISPR array (with spacer for AvPSP1)
AvCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
AvCAST Tns proteins (TnsA, TnsB, TnsC and TniQ)
AvTnsD

Expression

Bacterial

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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CSAC-Pers

Plasmid

#168281

Purpose

Expresses sgRNA in mammalian cells

Depositor

Han Kyoung Choe

Article

Kim et al Sci Rep. 2021 Jan 28;11(1):2575. doi: 10

Insert

hU6-sgPer1-3-hU6-sgPer2-1-hU6-sgPer2-2

Use

AAV

Tags

mCherry

Promoter

U6, hSyn

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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CSAC-Crys

Plasmid

#164967

Purpose

Expresses sgRNA in mammalian cells

Depositor

Han Kyoung Choe

Article

Kim et al Sci Rep. 2021 Jan 28;11(1):2575. doi: 10

Insert

hU6-sgCry1_2-hU6-sgCry2_1-hU6-sgCry2_2

Use

AAV

Tags

mCherry

Promoter

hU6, hSyn

Available Since

May 4, 2021

Availability

Academic Institutions and Nonprofits only

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pGIPz-PD-L1 4NQ

Plasmid

#121487

Purpose

Expression of human PD-L1 4NQ with PD-L1 shRNA

Depositor

Mien-Chie Hung

Article

Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

Inserts

PD-L1 4NQ
hPD-L1 shRNA

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

N35Q, N192Q, N200Q and N219Q

Promoter

CMV

Available Since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX4T1-Rpb4/7

Plasmid

#138484

Purpose

Expresses GST-tagged Rpb4 and His-tagged Rpb7 in bacteria

Depositor

Britt Glaunsinger

Article

Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

Inserts

Rpb4 (POLR2D Human)
Rpb7 (POLR2G Human)

Tags

6xHis and GST

Expression

Bacterial

Promoter

tac

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-syn-FLEX-synaptoPAC-minWPRE

Plasmid

#153100

Purpose

Cre-conditional expression of synaptoPAC, a fusion of synaptophysin, mScarlet, and bPAC in neurons

Depositor

Dietmar Schmitz

Article

Oldani et al J Neurochem. 2021 Feb;156(3):324-336. do

Inserts

bPAC(WT)
Synaptophysin (Syp Rat)

Use

AAV

Tags

His tag, mScarlet, and myc tag

Expression

Mammalian

Available Since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pCyCEN_Lisp2mCherry_hsp70_GFP

Plasmid

#137169

Purpose

Dual fluorescent reporter for liver stage expression in Plasmodium cynomolgi

Depositor

Clemens Kocken

Article

Voorberg-van der Wel et al Commun Biol. 2020 Jan 3;3:7. doi: 10.103

Inserts

mCherry
Nanoluc
dihydrofolate reductase
Green Fluorescent Protein

Use

Unspecified

Tags

T2A

Promoter

P. cynomolgi hsp70 and P. cynomolgi lisp2

Available Since

May 11, 2020

Availability

Academic Institutions and Nonprofits only
pGL3-Basic-hCYP26A1P-E4-luciferase

Plasmid

#135592

Purpose

Short form DNA fragment of human CYP26A1gene promoter (E4) in pGL3-Basic-luc vector

Depositor

Catharine Ross

Article

Zhang et al Gene. 2010 Sep 15;464(1-2):32-43. doi: 1

Insert

Short form promoter of human CYP26A1 gene (E4)

Promoter

Human CYP26A1 promoter (Short Form, E4)

Available Since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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Anti-Nav1.1 Na+ channel [K74/71R]

Plasmid

#128626

Purpose

Mammalian Expression Plasmid of anti-Nav1.1 Na+ channel (Rat). Derived from hybridoma K74/71.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Nav1.1 Na+ channel (Rat) recombinant mouse monoclonal antibody (Scn1a Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pB-RTP1S-Integration

Plasmid

#129458

Purpose

piggyBac transposon vector that Inducibly (Tet-On) expresses RTP1S

Depositor

Sriram Kosuri

Article

Jones et al Cell Syst. 2019 Mar 27;8(3):254-260.e6.

Insert

RTP1 (Rtp1 Mouse)

Use

Synthetic Biology

Expression

Mammalian

Mutation

Removal of the first 36 amino acids of the protein

Promoter

TRE (Tet-On)

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pSLQ7748 mU6: Spy sgTET || CMV: mCherry~P2A-DD-A4

Plasmid

#123657

Purpose

DD-AcrIIA4 fusion for Shield1-mediated AcrIIA4 control with sgRNA driving TRE3G activation.

Depositor

Stanley Qi

Article

Nakamura et al Nat Commun. 2019 Jan 14;10(1):194. doi:

Insert

mCherry-P2A-DD-AcrIIA4

Use

CRISPR, Lentiviral, and Synthetic Biology

Tags

mCherry-P2A

Expression

Mammalian

Promoter

CMV and mU6

Available Since

April 24, 2019

Availability

Academic Institutions and Nonprofits only

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pROS13

Plasmid

#107927

Purpose

kan based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

Depositor

Jean-Marc Daran

Article

Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

Insert

gRNA-CAN1.Y, gRNA-ADE2.Y

Use

CRISPR

Expression

Yeast

Available Since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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