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Showing: 761 - 780 of 11026 results

  7. LV-indLS2

    Plasmid
    #123068
    Purpose
    Lentiviral construct that delivers a tamoxifen inducible Cre. Upon recombination, luc2 and mStrawberry express. Used to image spontaneous tumorigenesis or subclonal cell populations in vivo.
    Depositor
    Kevin Brindle
    Article
    Rodriguez et al J Pathol. 2014 Mar;232(4):449-57. doi: 1
    Inserts
    CreERT2 with intron
    Firefly luciferase
    mStrawberry
    Use
    Lentiviral and Luciferase; Fluorescence
    Tags
    Expression
    Mammalian
    Mutation
    synthetic intron added as indicated
    Promoter
    CAGGS (after Cre mediated inversion) and CAGGS (f…
    Available since
    April 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    123068-plasmid-map-sequence-id-240523

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  11. SGyA

    Plasmid
    #160292
    Purpose
    Drives expression of Cas9 and a TdTomato fluorescent marker
    Depositor
    Omar Akbari
    Article
    Gamez et al Nat Commun. 2021 Dec 10;12(1):7202. doi:
    Inserts
    Vas promoter
    hSpCas9
    tdTomato
    CTCF insulator
    Gypsy insulator
    Left homology arm Y chromosome
    Right homology arm Y chromosome
    Use
    Tags
    T2A GFP
    Expression
    Insect
    Mutation
    Promoter
    Available since
    March 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    160292-plasmid-map-sequence-id-316542

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  12. prk5-MFSD12-TEV-3xFLAG-3xHA PM mutant

    Plasmid
    #149507
    Purpose
    Transient expression of human MFSD12 with recoded nucleotide sequence. Dileucine motif LL256-257 has been mutated to AA.
    Depositor
    David Sabatini
    Article
    Adelmann et al Nature. 2020 Nov 18. pii: 10.1038/s41586
    Insert
    MFSD12 (MFSD12 Human)
    Use
    Transient expression
    Tags
    TEV-3XHA-3XFLAG
    Expression
    Mammalian
    Mutation
    Codon Optimized Sequence, p.LL256-257AA
    Promoter
    CMV
    Available since
    Nov. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    149507-plasmid-map-sequence-id-317190

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  13. pCR4-Dact1(1250-1601)

    Plasmid
    #52046
    Purpose
    To generate mRNA in situ probe against mouse Dact1 (Probe C in PMID 17013874). For antisense cut with Spe1, transcribe with T7. For sense cut with Not1, treat with T4 polymerase, transcribe with T3.
    Depositor
    Ben Cheyette
    Article
    Suriben et al Dev Dyn. 2006 Nov;235(11):3177-83.
    Insert
    Dact1(1250-1601) (Dact1 Mouse)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 18, 2014
    Availability
    Academic Institutions and Nonprofits only

  14. pWR63(YUM1)

    Plasmid
    #203336
    Purpose
    episomal Cas9 and YUM1-targeting sgRNA expression vector for S. stipitis
    Depositor
    J. Brian Robertson
    Article
    Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:
    Inserts
    TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
    coHPH
    ACT1 terminator (S. stipitis)
    CCW12 promoter (S. stipitis)
    CaCas9
    ADH2 terminator (S. stipitis)
    ARS from S. stipitis chromosome 1
    THD3 promoter (S. stipitis)
    tRNA-sgRNA(SapI)-HDV
    ADH1 terminator (S. stipitis)
    YUM1-targeting sequence
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    June 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    203336-plasmid-map-sequence-id-417292

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  15. pWR63

    Plasmid
    #203331
    Purpose
    episomal Cas9 and sgRNA expression vector without sgRNA for S. stipitis
    Depositor
    J. Brian Robertson
    Article
    Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:
    Inserts
    TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
    coHPH
    ACT1 terminator (S. stipitis)
    CCW12 promoter (S. stipitis)
    CaCas9
    ADH2 terminator (S. stipitis)
    ARS from S. stipitis chromosome 1
    THD3 promoter (S. stipitis)
    tRNA-sgRNA(SapI)-HDV
    ADH1 terminator (S. stipitis)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    June 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    203331-plasmid-map-sequence-id-409751

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  19. pLJM1-Lyso-FLAG-GFP-Sac1-Cat-WT

    Plasmid
    #134645
    Purpose
    lysosome-targeted Sac1 catalytic domain wild type
    Depositor
    Roberto Zoncu
    Article
    Lim et al Nat Cell Biol. 2019 Oct;21(10):1206-1218
    Insert
    Sac1 catalytic domain (77-520 aa) (SACM1L Human)
    Use
    Lentiviral
    Tags
    FLAG, GFP, and lysosomal-tag (p18 N-terminal 1-39…
    Expression
    Mammalian
    Mutation
    contains only amino acids 77-520 (Please see depo…
    Promoter
    Available since
    Dec. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    134645-plasmid-map-sequence-id-266496

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  20. pLJM1-Lyso-FLAG-GFP-Sac1-Cat-CS

    Plasmid
    #134653
    Purpose
    lysosome-targeted Sac1 catalytic domain C389S
    Depositor
    Roberto Zoncu
    Article
    Lim et al Nat Cell Biol. 2019 Oct;21(10):1206-1218
    Insert
    Sac1 catalytic domain (77-520 aa) CS mutant (SACM1L Human)
    Use
    Lentiviral
    Tags
    FLAG, GFP, and lysosomal-tag (p18 N-terminal 1-39…
    Expression
    Mammalian
    Mutation
    C389S and contains only amino acids 77-520 (Pleas…
    Promoter
    Available since
    Dec. 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    134653-plasmid-map-sequence-id-266494

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Showing: 761 - 780 of 11026 results