We narrowed to 3,870 results for: SPL

1. pFP.R271

   Plasmid

   #138220

   Purpose

   Expresses the split mCherry reporter interrupted by the SspDnaX artificially mini-intein (in cis)

   Depositor

   Baojun Wang

   Article

   Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

   Inserts

   araC (araC )
   mCherryN(IDE).SspDnaX.(CHT)mCherryC.H6
   

   Use

   Synthetic Biology

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   P(araBAD) and iPC

   Available since

   May 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. plIVMD164

   Plasmid

   #160936

   Purpose

   In vivo mRNA display construct for GFP

   Depositor

   Saeed Tavazoie

   Article

   Oikonomou et al Proc Natl Acad Sci U S A. 2020 Oct 9. pi

   Insert

   MCP-sfGFP-HIS tag, 3'UTR MS2 Stem Loop
   

   Use

   Tags

   HIS tag, 3'UTR MS2 Stem Loop

   Expression

   Yeast

   Mutation

   Promoter

   MET25

   Available since

   Jan. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. P16ΔS:(MCS)-3xHA-S11

   Plasmid

   #108185

   Purpose

   Binary vectors used for the constitutive expression of tripartite split-sfGFP components (with a 3xHA tag) in plants

   Depositor

   Tzu-Yin Liu

   Article

   Liu et al Plant J. 2018 Feb 16. doi: 10.1111/tpj.1

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   P16ΔS

   Available since

   May 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFP.R365

   Plasmid

   #138235

   Purpose

   Expresses the split mCherry reporter interrupted by the EP-Pri artificially mini-intein (in cis)

   Depositor

   Baojun Wang

   Article

   Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

   Inserts

   araC (araC )
   mCherryN(HGK).EP-Pri*.(TEV)mCherryC.H6
   

   Use

   Synthetic Biology

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   P(araBAD) and iPC

   Available since

   March 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. 3XFLAG-NSarQ27G-VMAn-FKBP

   Plasmid

   #70229

   Purpose

   Expresses FLAG tagged N-terminal portion of alpha sarcin with Q27G mutation along with VMA intein for protein splicing

   Depositor

   Perry Howard

   Article

   Alford et al Mol Biosyst. 2014 Apr;10(4):831-7. doi:

   Insert

   alpha Sarcin (N-terminal portion)
   

   Use

   Tags

   3XFLAG, FK506- and rapamycin-binding protein (FKB…

   Expression

   Mammalian

   Mutation

   Changed Gln 27 to Gly

   Promoter

   CMV

   Available since

   Nov. 24, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pCI-SMN2-inclusion-GLuc

   Plasmid

   #218671

   Purpose

   Alternative splicing signaling: signal increased with Ex7 inclusion increasing

   Depositor

   Jingxin Wang

   Article

   Tang et al Chembiochem. 2022 May 4;23(9):e202200012

   Insert

   SMN2 (SMN2 )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. psiT-GEM(0)

   Plasmid

   #220446

   Purpose

   Split intein version of Trojan-Gal4 Expression Module in Phase 0. Insert T2A-Gal4 to genomic loci using Crispr/Cas technology. Can be converted by cassette exchange into other Trojan exons

   Depositor

   Benjamin White

   Article

   Diao et al Proc Natl Acad Sci U S A. 2024 Apr 23;12

   Insert

   CfaN-Gal4-CfaC
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Aug. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. psiT-GEM(2)

   Plasmid

   #220448

   Purpose

   Split intein version of Trojan-Gal4 Expression Module in Phase 2. Insert T2A-Gal4 to genomic loci using Crispr/Cas technology. Can be converted by cassette exchange into other Trojan exons

   Depositor

   Benjamin White

   Article

   Diao et al Proc Natl Acad Sci U S A. 2024 Apr 23;12

   Insert

   CfaN-Gal4-CfaC
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   July 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. psiT-GEM(1)

   Plasmid

   #220447

   Purpose

   Split intein version of Trojan-Gal4 Expression Module in Phase 1. Insert T2A-Gal4 to genomic loci using Crispr/Cas technology. Can be converted by cassette exchange into other Trojan exons

   Depositor

   Benjamin White

   Article

   Diao et al Proc Natl Acad Sci U S A. 2024 Apr 23;12

   Insert

   CfaN-Gal4-CfaC
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   June 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMS18

    Plasmid

    #215675

    Purpose

    Cas9 + guide plasmid for inserting ChrI split hygromycinR landing pad

    Depositor

    Patrick Phillips

    Article

    Moerdyk-Schauwecker et al MicroPubl Biol. 2024 Jan 29;2024:10.1791

    Insert

    eef-1A.1p::Cas9 + U6p::GAAATCGCCGACTTGCGAGG
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMS62

    Plasmid

    #215676

    Purpose

    Cas9 + guide plasmid for inserting ChrIII split hygromycinR landing pad

    Depositor

    Patrick Phillips

    Article

    Moerdyk-Schauwecker et al MicroPubl Biol. 2024 Jan 29;2024:10.1791

    Insert

    eef-1A.1p::Cas9 + U6p::GTCGTTCTTCCGTTCTCGGG
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMS77

    Plasmid

    #215681

    Purpose

    Cas9 + guide plasmid targeting ChrIII split hygromycinR landing pad

    Depositor

    Patrick Phillips

    Article

    Moerdyk-Schauwecker et al MicroPubl Biol. 2024 Jan 29;2024:10.1791

    Insert

    eef-1A.1p::Cas9 + U6p::GTCCAGCGGCAGATCGGCGG
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    March 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pYSD063

    Plasmid

    #212916

    Purpose

    Encodes mRuby2 fluorescent protein as a Type 3b' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)

    Depositor

    Paul Young

    Article

    O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

    Insert

    mRuby2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. NODALvar-MYC-DYK-C302S

    Plasmid

    #115254

    Purpose

    For mammalian expression of a human NODAL splice variant open reading frame (with a mutated Cysteine residue) with a C-terminal MYC-DYK tag

    Depositor

    Lynne Postovit

    Article

    Findlay et al (2018) bioRxiv 276170

    Insert

    NODAL (NODAL Human)
    

    Use

    Tags

    MYC-DYK

    Expression

    Mammalian

    Mutation

    C302S

    Promoter

    CMV

    Available since

    Sept. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. NODALvar-MYC-DYK-C302A

    Plasmid

    #115255

    Purpose

    For mammalian expression of a human NODAL splice variant open reading frame (with a mutated Cysteine residue) with a C-terminal MYC-DYK tag

    Depositor

    Lynne Postovit

    Article

    Findlay et al (2018) bioRxiv 276170

    Insert

    NODAL (NODAL Human)
    

    Use

    Tags

    MYC-DYK

    Expression

    Mammalian

    Mutation

    C302A

    Promoter

    CMV

    Available since

    Sept. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. plIVMD163

    Plasmid

    #160935

    Purpose

    In vivo mRNA display construct for mCherry

    Depositor

    Saeed Tavazoie

    Article

    Oikonomou et al Proc Natl Acad Sci U S A. 2020 Oct 9. pi

    Insert

    MCP-mCherry-HIS tag, 3'UTR MS2 Stem Loop
    

    Use

    Tags

    HIS tag, 3'UTR MS2 Stem Loop

    Expression

    Yeast

    Mutation

    Promoter

    MET25

    Available since

    Nov. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pFP.R373

    Plasmid

    #138243

    Purpose

    Expresses the split mCherry reporter interrupted by the SaP-dpol intein (in cis)

    Depositor

    Baojun Wang

    Article

    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

    Inserts

    araC (araC )
    mCherryN(GKA).SaP-dpol*.(CEL)mCherryC.H6
    

    Use

    Synthetic Biology

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    P(araBAD) and iPC

    Available since

    July 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pFP.R362

    Plasmid

    #138232

    Purpose

    Expresses the split mCherry reporter interrupted by the CbP-RNR artificially mini-intein (in cis)

    Depositor

    Baojun Wang

    Article

    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

    Inserts

    araC (araC )
    mCherryN(ASC).CbP-RNR*.(CRL)mCherryC.H6
    

    Use

    Synthetic Biology

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    P(araBAD) and iPC

    Available since

    May 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pFP.R376

    Plasmid

    #138246

    Purpose

    Expresses the split mCherry reporter interrupted by the Pfu-RIR1-1 artificially mini-intein (in cis)

    Depositor

    Baojun Wang

    Article

    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

    Inserts

    araC (araC )
    mCherryN(GGG).Pfu-RIR1-1*.(TGL)mCherryC.H6
    

    Use

    Synthetic Biology

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    P(araBAD) and iPC

    Available since

    May 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pFP.R363

    Plasmid

    #138233

    Purpose

    Expresses the split mCherry reporter interrupted by the Ckl-Ter artificially mini-intein (in cis)

    Depositor

    Baojun Wang

    Article

    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

    Inserts

    araC (araC )
    mCherryN(NGK).Ckl-Ter*.(TTL)mCherryC.H6
    

    Use

    Synthetic Biology

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    P(araBAD) and iPC

    Available since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge