We narrowed to 1,112 results for: cas12a

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pLCHKO

Plasmid

#155048

Purpose

Lentiviral backbone for cloning and expressing U6 driven hybrid guide (hg)RNAs with BfuAI cloning sites and puromycin selection.

Depositor

Benjamin Blencowe, Jason Moffat

Publication

Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available since

Aug. 12, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pYPQ230-E795L

Plasmid

#176890

Purpose

Gateway entry plasmid (attL1 & attR5) expressing LbCas12a with E795L mutation, without promoter

Depositor

Yiping Qi

Publication

Zhang et al Front Genome Ed. 2022 Jan 31;4:780238. d

Insert

LbCas12a-E795L

Use

CRISPR; Gateway compatible lbcpf1 entry clone

Tags

Nucleoplasmin NLS and SV40 NLS

Expression

Plant

Available since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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pUC19-U6-crRNA1

Plasmid

#123639

Purpose

Mammalian expression, Genome editing, gRNA scaffold

Depositor

Wei Li

Publication

Teng et al Genome Biol. 2019 Feb 5;20(1):15. doi: 1

Insert

crRNA scaffold 1

Expression

Mammalian

Available since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pYPQ142-AtUBQ10

Plasmid

#250923

Purpose

Golden Gate recipient and Gateway entry vector; assembly of 2 gRNAs driven by AtUBQ10 promoter

Depositor

Yiping Qi

Publication

Cheng et al J Integr Plant Biol. 2026 Apr 2. doi: 10

Insert

AtUBQ10

Use

CRISPR

Expression

Plant

Available since

April 17, 2026

Availability

Academic Institutions and Nonprofits only

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pYPQ144-AtUBQ10-pT

Plasmid

#250924

Purpose

Golden Gate recipient and Gateway entry vector; assembly of 4 gRNAs driven by AtUBQ10 promoter

Depositor

Yiping Qi

Publication

Cheng et al J Integr Plant Biol. 2026 Apr 2. doi: 10

Insert

AtUBQ10

Use

CRISPR

Expression

Plant

Available since

April 17, 2026

Availability

Academic Institutions and Nonprofits only

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pYPQ143-AtUBQ10

Plasmid

#250985

Purpose

Golden Gate recipient and Gateway entry vector; assembly of 3 gRNAs driven by AtUBQ10 promoter

Depositor

Yiping Qi

Publication

Cheng et al J Integr Plant Biol. 2026 Apr 2. doi: 10

Insert

AtUBQ10

Use

CRISPR

Expression

Plant

Available since

April 17, 2026

Availability

Academic Institutions and Nonprofits only

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pJCC_084 Cas12i1 D647A

Plasmid

#171671

Purpose

For bacterial expression of Cas12i1 D647A (RuvC-inhibiting mutation) with an N-terminal His-MBP tag

Depositor

Jennifer Doudna

Publication

Cofsky et al Elife. 2020 Jun 10;9. pii: 55143. doi: 1

Insert

Cas12i1 D647A

Tags

10X His, TEV protease cleavage site, and maltose-…

Expression

Bacterial

Mutation

changed aspartate 647 to alanine

Available since

June 16, 2021

Availability

Academic Institutions and Nonprofits only

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pMaCTag-21

Plasmid

#120000

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with 24xSunTag

Depositor

Michael Knop

Publication

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

24xSunTag

Use

Pcr template

Tags

24xSunTag

Promoter

None

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-Z27

Plasmid

#120070

Purpose

Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with 1xHA

Depositor

Michael Knop

Publication

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

1xHA

Use

Pcr template

Tags

1xHA

Promoter

None

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-Z24

Plasmid

#120067

Purpose

Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with TAP

Depositor

Michael Knop

Publication

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

TAP

Use

Pcr template

Tags

TAP

Promoter

None

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-Z10

Plasmid

#120053

Purpose

Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mKOk

Depositor

Michael Knop

Publication

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mKOk

Use

Pcr template

Tags

mKOk

Promoter

None

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-Z01

Plasmid

#120044

Purpose

Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with moxBFP

Depositor

Michael Knop

Publication

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

moxBFP

Use

Pcr template

Tags

moxBFP

Promoter

None

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-P16

Plasmid

#120027

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with Dronpa3

Depositor

Michael Knop

Publication

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

Dronpa3

Use

Pcr template

Tags

Dronpa3

Promoter

None

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-31

Plasmid

#120010

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with V5

Depositor

Michael Knop

Publication

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

V5

Use

Pcr template

Tags

V5

Promoter

None

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-20

Plasmid

#119999

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with 10xSuntag

Depositor

Michael Knop

Publication

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

10xSuntag

Use

Pcr template

Tags

10xSuntag

Promoter

None

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-16

Plasmid

#119995

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with Dronpa3

Depositor

Michael Knop

Publication

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

Dronpa3

Use

Pcr template

Tags

Dronpa3

Promoter

None

Available since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-03

Plasmid

#119982

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with moxCerulean3

Depositor

Michael Knop

Publication

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

moxCerulean3

Use

Pcr template

Tags

moxCerulean3

Promoter

None

Available since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-02

Plasmid

#119981

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with TagBFP

Depositor

Michael Knop

Publication

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

TagBFP

Use

Pcr template

Tags

TagBFP

Promoter

None

Available since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pSLQ10714

Plasmid

#183959

Purpose

U6-crKlf4-CAG-hyperdCas12a-miniVPR

Depositor

Stanley Qi

Publication

Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Inserts

HyperdCas12a
crRNA targeting Klf4

Use

CRISPR

Tags

HA

Expression

Mammalian

Mutation

D832A, D156R, E292R, D235R, D350R

Promoter

CAG and U6

Available since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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pGRACE

Plasmid

#230910

Purpose

Tracing of CRISPR activity in whole animals.

Depositor

Michael Boutros

Publication

Port et al Nat Commun. 2026 Jan 15;17(1):877. doi:

Insert

GFP

Use

CRISPR

Expression

Insect

Available since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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