We narrowed to 5,701 results for: chia

1. pLD1 translation factors

   Plasmid

   #117760

   Purpose

   BioBrick encoding cistrons 1-13 in pET for preparation of PURE 3.0 translation system from E. coli

   Depositor

   Anthony Forster

   Article

   Shepherd et al Nucleic Acids Res. 2017 Oct 13;45(18):10

   Insert

   Translation factor cistrons 1-13
   

   Use

   Escherichia coli

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLD3 translation factors

   Plasmid

   #117762

   Purpose

   BioBrick encoding cistrons 22-30 in pET for preparation of PURE 3.0 translation system from E. coli

   Depositor

   Anthony Forster

   Article

   Shepherd et al Nucleic Acids Res. 2017 Oct 13;45(18):10

   Insert

   Translation factor cistrons 22-30
   

   Use

   Escherichia coli

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLD2 translation factors

   Plasmid

   #117761

   Purpose

   BioBrick encoding cistrons 14-21 in pET for preparation of PURE 3.0 translation system from E. coli

   Depositor

   Anthony Forster

   Article

   Shepherd et al Nucleic Acids Res. 2017 Oct 13;45(18):10

   Insert

   Translation factor cistrons 14-21
   

   Use

   Escherichia coli

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET24a-AlkB deltaN11

   Plasmid

   #73622

   Purpose

   Expresses E. coli AlkB absent the first 11 amino acids and with a C-terminal histidine tag

   Depositor

   Catherine Drennan

   Article

   Frick et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

   Insert

   AlkB (alkB E. coli)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   deletion of first 11 AA

   Promoter

   T7

   Available since

   Feb. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

5. AIO-mCherry

   Plasmid

   #74120

   Purpose

   All-in-One plasmid encoding dual U6 promoter-driven sgRNAs and mCherry-coupled Cas9-D10A nickase to enhance efficient and accurate genome editing

   Depositor

   Steve Jackson

   Article

   Chiang et al Sci Rep. 2016 Apr 15;6:24356. doi: 10.10

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. LifeAct-GFP-cytoB5RR

   Plasmid

   #182577

   Purpose

   Expresses LifeAct-GFP targeted to the ER via the CytB5RR tail anchor sequence

   Depositor

   Uri Manor

   Article

   Schiavon et al Nat Methods. 2020 Sep;17(9):917-921. doi

   Insert

   LifeAct-GFP-cytoB5RR
   

   Use

   Tags

   LifeAct and cytoB5RR

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEP PolI

   Plasmid

   #11722

   Depositor

   Lawrence Loeb

   Article

   Camps et al Proc Natl Acad Sci U S A. 2003 Aug 19. 1

   Insert

   DNA polymerase I EP (polA E. coli)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   D424A, I709N, A759R. Error-prone version of PolI.

   Promoter

   Available since

   Aug. 8, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJL017

   Plasmid

   #53532

   Purpose

   This plasmid encodes both the etpB and etpA two partner secretion genes on an arabinose-inducible plasmid with myc and 6His tags in-frame with the etpA gene

   Depositor

   James Fleckenstein

   Article

   Fleckenstein et al Nat Protoc. 2009;4(7):1083-92. doi: 10.1

   Insert

   etpBA
   

   Use

   Tags

   6His and myc

   Expression

   Bacterial

   Mutation

   Promoter

   pBAD

   Available since

   June 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pKanB alpha

   Plasmid

   #223524

   Purpose

   Used for expression of secreted recombinant protein in Pichia pastoris; with G418 resistance gene as selectable marker

   Depositor

   Geoff Lin-Cereghino

   Article

   Lin-Cereghino et al Yeast. 2008 Apr;25(4):293-9. doi: 10.100

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   AOX1

   Available since

   Sept. 11, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pA5c-tesA-vhb50-8fadR

    Plasmid

    #86601

    Purpose

    expression of tesA and Vhb50 under the control of a LacUV5 promoter and fadR under the control of a pBAD promoter

    Depositor

    Yinjie Tang

    Article

    Liu et al Biotechnol Bioeng. 2016 Aug 6. doi: 10.1

    Inserts

    tesA
    Vhb50
    fadR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    LacUV5 and pBAD

    Available since

    Jan. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMAL-EFSep

    Plasmid

    #34627

    Depositor

    Jesse Rinehart, Dieter Söll

    Article

    Park et al Science. 2011 Aug 26;333(6046):1151-4.

    Insert

    EF-Sep (tufB E. coli)
    

    Use

    Tags

    6xHIS and MBP

    Expression

    Bacterial

    Mutation

    His67 to Arg , Glu216 to Asn, Asp217 to Gly, Phe2…

    Promoter

    Available since

    March 1, 2012

    Availability

    Academic Institutions and Nonprofits only

12. pTJV1Sc3-rpoB

    Plasmid

    #166983

    Purpose

    Generates ssDNA in vivo targeting E. coli rpoB for recombineering w/ EcRecT recombinase; temp. sensitive pSC101 ori and sgRNA for Cas9 counterselection; aada for spectinomycin resistance

    Depositor

    Christopher Reisch

    Article

    Ellington et al Synth Biol (Oxf). 2022 May 3;7(1):ysac00

    Insert

    RecT (recT E. coli)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pVvanCC

    Available since

    May 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGBW-m4046852

    Plasmid

    #145584

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 nsp8

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 nsp8 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;6xHIS

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 28, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pKB817 (pBra-Hfq)

    Plasmid

    #174661

    Purpose

    Plpp/PlacUV5- directed synthesis of the alpha NTD fused to E. coli Hfq. Useful as three-hybrid positive control.

    Depositor

    Katherine Berry, Ann Hochschild

    Article

    Berry et al Nucleic Acids Res. 2018 Jan 25;46(2):e12

    Insert

    Hfq
    

    Use

    Tags

    alpha NTD

    Expression

    Bacterial

    Mutation

    Promoter

    lpp/lacUV5 (tandem promoter)

    Available since

    May 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGBW-m4046979

    Plasmid

    #145611

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 nsp7

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 nsp7 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;6xHIS

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 28, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pGBW-m4046920

    Plasmid

    #145717

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 nsp3

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 nsp3 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;6xHIS

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 27, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. RIB VP64 R1

    Plasmid

    #161493

    Purpose

    CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pGAP_RIB1_R1_gRNA_MS2

    Depositor

    Diethard Mattanovich, Brigitte Gasser

    Article

    Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

    Inserts

    dCAS9
    MS2-VP64
    gRNA (Targeting R1 in the RIB1 promoter)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pGAP, pPOR1, and pTEF2

    Available since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGBW-m4046760

    Plasmid

    #145600

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 helicase

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 helicase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;6xHIS

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pGBW-m4046814

    Plasmid

    #145601

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 E (envelope)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 E (envelope) (E Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;6xHIS

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 28, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pCfuNca-Plastid

    Plasmid

    #190818

    Purpose

    Plastid targeting GFP with ble resistant gene for zeocin

    Depositor

    Peter Kroth

    Article

    Sprecher et al Algal Research. 2023 May; Vol 72: 103136

    Inserts

    Diatom plastid targeted GFP
    Bleomycin resistance gene
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Cylindrotheca fusiformis fcp and Nitzschia captiv…

    Available since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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