We narrowed to 19,717 results for: Comp

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pJL VanS NarX Chimera

Plasmid

#225655

Purpose

Expresses a NarX-based chimera capable of sensing vancomycin. For use with NarL-YdfI and reporter plasmids from this study.

Depositor

Neha Kamat

Article

Peruzzi et al Proc Natl Acad Sci U S A. 2023 May 9;120

Insert

VanS-NarX

Use

Cell free expression

Tags

Myc

Expression

Bacterial

Mutation

Transmembrane and sensing domain replaced to dete…

Promoter

T7

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO GFP HSPH1

Plasmid

#19480

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

HSPH1 (HSPH1 Human)

Tags

GFP

Expression

Mammalian

Available Since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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pcDNA-GFP-BIRC3-LU

Plasmid

#160700

Purpose

Expresses GFP-BIRC3 with the long 3'UTR in mammalian cells

Depositor

Christine Mayr

Article

Lee et al Mol Cell. 2019 May 16;74(4):701-712.e9.

Insert

GFP-BIRC3 (BIRC3 Human)

Expression

Mammalian

Available Since

April 26, 2021

Availability

Academic Institutions and Nonprofits only

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FHpHG_mTent5a

Plasmid

#221439

Purpose

Transfection into the cells for the reporter or qPCR assay.

Depositor

Marcos Morgan

Article

Li et al FEBS J. 2025 May 27. doi: 10.1111/febs.7

Insert

Tent5a (Fam46a Mouse)

Tags

FLAG-2xHA-(HRV 3C site)-6xHis-EGFP

Expression

Mammalian

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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pP19 (GB0038)

Plasmid

#68195

Purpose

Provides the CDS of the Tomato bushy stunt virus (TBSV) silencing suppressor P19 as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

P19 coding region

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pSLQ2840 pPB: CAG-Puro-WPRE PGK-VPR-tagBFP-SadCas9

Plasmid

#84246

Purpose

Expresses direct fusion VPR-Sa dCas9

Depositor

Stanley Qi

Article

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Inserts

VPR-tagBFP-Sa dCas9
Puro

Use

CRISPR and Synthetic Biology; Piggybac

Tags

Nucleoplasmin NLS, SV40 NLS, VPR, and tagBFP

Expression

Mammalian

Promoter

CAG and PGK

Available Since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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pEGFP_IRES_tdTomato

Plasmid

#184046

Purpose

Expresses EGFP and tdTomato in mammalian cells under control of a CMV promoter by using an IRES sequence

Depositor

Angela Pannier

Article

Kozisek et al J Biol Eng. 2023 Dec 7;17(1):76. doi: 10

Inserts

Enhanced Green Fluorescent Protein
tdTomato
Internal Ribosome Entry Site

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pHIE050 ZF43x0 mKate2 EF1a VP16-ZF43 EF1a EBFP2-P2A-BlastR

Plasmid

#138731

Purpose

Integration Vector, assembled with pHIE042 (TUPV1), pJM466 (TUPV2), pJM452 (TUPV3), pPD613

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43x0

Use

Synthetic Biology

Expression

Mammalian

Available Since

Jan. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Flag TSC2 (S939A/T1462A)

Plasmid

#14131

Depositor

Brendan Manning

Article

Manning et al Mol Cell. 2002 Jul . 10(1):151-62.

Insert

TSC2 S939A/T1462A (TSC2 Human)

Tags

Flag

Expression

Mammalian

Mutation

S939A/T1462A

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
pSLC-254

Plasmid

#124291

Purpose

Expression of vsfGFP-0. This construct improves brightness of sfGFP by fusing a GFP-specific single domain antibody to it that creates a dimeric fluorophore.

Depositor

Swaine Chen

Article

Eshaghi et al Angew Chem Int Ed Engl. 2015 Nov 16;54(4

Insert

vsfGFP-0

Expression

Bacterial

Available Since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pTP2620

Plasmid

#113311

Purpose

human Rad50 in baculovirus transfer vector

Depositor

Tanya Paull

Article

Lee et al J Biol Chem. 2013 May 3;288(18):12840-51

Insert

Rad50 (RAD50 Human)

Tags

HA, 6xhis

Expression

Insect

Promoter

polyhedrin

Available Since

Nov. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pCX-EGFP beta5 integrin receptor

Plasmid

#14996

Depositor

Raymond Birge

Article

Albert et al Nat Cell Biol. 2000 Dec . 2(12):899-905.

Insert

beta5 integrin receptor (ITGB5 Human)

Tags

IRES EGFP

Expression

Mammalian

Available Since

Aug. 22, 2007

Availability

Academic Institutions and Nonprofits only

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pME-loxP-mTagBFP2-stop-pA-loxP

Plasmid

#75158

Purpose

Floxed TagBFP2 (blue) fluorophore with stop codon for inducible lines.

Depositor

Nicholas Cole

Article

Don et al Zebrafish. 2016 Sep 15.

Insert

loxP-mTagBFP2-stop-pA-loxP

Use

Middle entry vector for multisite gateway three f…

Promoter

n/a

Available Since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pCARGO-RMCE-Xist

Plasmid

#233268

Purpose

CARGO plasmid containing ~22kb dox-inducible Xist transgene

Depositor

Mauro Calabrese

Article

Trotman et al Mol Cell. 2025 Mar 20;85(6):1117-1133.e1

Insert

22kb Xist gene, including introns

Expression

Mammalian

Available Since

March 28, 2025

Availability

Academic Institutions and Nonprofits only

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pSLQ2815 pPB: CAG-Puro-WPRE PGK-KRAB-tagBFP-SpdCas9

Plasmid

#84245

Purpose

Expresses direct fusion KRAB-Sp dCas9

Depositor

Stanley Qi

Article

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Inserts

KRAB-tagBFP-Sp dCas9
Puro

Use

CRISPR and Synthetic Biology; Piggybac

Tags

2xNLS (SV40), HA tag, KRAB, and tagBFP

Expression

Mammalian

Promoter

CAG and PGK

Available Since

Nov. 9, 2016

Availability

Academic Institutions and Nonprofits only

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MCP-ADAR2-DD(E488Q)

Plasmid

#170125

Purpose

Lentiviral vector carrying the MCP-ADAR2(E488Q) with a nuclear export signal

Depositor

Prashant Mali

Article

Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

Insert

MCP-ADAR2-DD(E488Q)-NES

Use

Lentiviral

Expression

Mammalian

Promoter

Ef1a core promoter

Available Since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pME18s-HA-hFIP200

Plasmid

#24303

Depositor

Noboru Mizushima

Article

Hosokawa et al Mol Biol Cell. 2009 Apr . 20(7):1981-91.

Insert

FIP200 (RB1CC1 Human)

Tags

3xHA

Expression

Mammalian

Available Since

June 24, 2010

Availability

Academic Institutions and Nonprofits only

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pEBTet-β-tubulin_SNAP

Plasmid

#136831

Purpose

Mammalian expression of the centrosomal protein _-tubulin C-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

TUBB_SNAP (TUBB Human, Synthetic)

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

April 6, 2020

Availability

Academic Institutions and Nonprofits only

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pDARMO_CMVT_3xFLAG_BIRC6

Plasmid

#197967

Purpose

Mammalian cell expression of wt 3xFLAG-BIRC6

Depositor

Eric Fischer

Article

Hunkeler et al Science. 2023 Mar 17;379(6637):1105-1111

Insert

BIRC6 (BIRC6 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

L1332V

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pFRT-TO-DEST

Plasmid

#106348

Purpose

Destination vector for gateway cloning. Developed for Flp-In T-REx system.

Depositor

Thomas Tuschl

Article

Yamaji et al Nature. 2017 Mar 23;543(7646):568-572. d

Type

Empty backbone

Use

Gateway entry vector

Expression

Mammalian

Available Since

March 26, 2018

Availability

Academic Institutions and Nonprofits only

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