We narrowed to 23,148 results for: REP

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pQC.Flip-nluc(LgBiT1-8)CP[Casp3A(D5A)]-Fluc

Plasmid

#200124

Purpose

Retroviral expression of Flip-nluc(LgBiT1-8)CP[Casp3A(D5A)] protease reporter and P2A firefly luciferase in mammalian cells

Depositor

Eiji Morita

Article

Arakawa et al Sci Rep. 2023 Jan 31;13(1):1753. doi: 10

Insert

Flip-nluc(LgBiT1-8)CP[Casp3A(D5A)] P2A firefly luciferase

Use

Retroviral

Expression

Mammalian

Promoter

CMV

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pQC.Flip-nluc(LgBiT1-8)CP[CoVA]-Fluc

Plasmid

#200125

Purpose

Retroviral expression of Flip-nluc(LgBiT1-8)CP[CoVA] protease reporter and P2A firefly luciferase in mammalian cells

Depositor

Eiji Morita

Article

Arakawa et al Sci Rep. 2023 Jan 31;13(1):1753. doi: 10

Insert

Flip-nluc(LgBiT1-8)CP[CoVA] P2A firefly luciferase

Use

Retroviral

Expression

Mammalian

Promoter

CMV

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV/CaMKIIa::iLMO2

Plasmid

#129481

Purpose

AAV2 transfer plasmid for iLMO2, an opto-chemogenetic probe for neuronal inhibition by light or chemical, under control of the glutamatergic-selective CaMKIIa promoter

Depositor

Robert Gross

Article

Tung et al Sci Rep. 2015 Sep 24;5:14366. doi: 10.10

Insert

iLMO2

Use

AAV

Expression

Mammalian

Promoter

CaMKIIa

Available Since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1_mNeongreen-DARPinOFF7

Plasmid

#182414

Purpose

Bacterial expression of mNeongreen-DARPinOFF7 fusion

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

mNeongreen-DARPinOFF7

Tags

mNeongreen

Expression

Bacterial

Promoter

T7

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1_mNeongreen-Uricase

Plasmid

#182416

Purpose

Bacterial expression of mNeongreen-uricase fusion

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

mNeongreen-uricase

Tags

mNeongreen

Expression

Bacterial

Promoter

T7

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1_mNeongreen-StefinA

Plasmid

#182418

Purpose

Bacterial expression of mNeongreen-stefinA fusion

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

mNeongreen-stefin (CSTA Human)

Tags

mNeongreen

Expression

Bacterial

Promoter

T7

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV6-ZNF354C_mutant_M41K-L42K-E43K

Plasmid

#194185

Purpose

Expresses ZNF354C with mutated KRAB domain (M41K, L42K, E43K mutation)

Depositor

Ralf Brandes, Matthias Leisegang

Article

Oo et al Sci Rep. 2020 Nov 5;10(1):19079. doi: 10

Insert

ZNF354C (ZNF354C Human)

Tags

Myc-DDK

Expression

Mammalian

Mutation

KRAB domain mutant M41K/L42K/E43K

Promoter

CMV

Available Since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pBABR mCherry-Bcd-LEXY

Plasmid

#182596

Purpose

Drosophila integration plasmid expressing mCherry-Bcd-LEXY fusion protein under the maternal tubulin promoter.

Depositor

Jared Toettcher

Article

Singh et al Cell Rep. 2022 Mar 22;38(12):110543. doi

Insert

Bcd (bcd Fly)

Tags

LEXY and mCherry

Expression

Insect

Available Since

Aug. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pBABR Bcd-LEXY

Plasmid

#182594

Purpose

Drosophila integration plasmid expressing Bcd-LEXY fusion protein under the maternal tubulin promoter.

Depositor

Jared Toettcher

Article

Singh et al Cell Rep. 2022 Mar 22;38(12):110543. doi

Insert

Bicoid (bcd Fly)

Tags

LEXY

Expression

Insect

Available Since

Aug. 22, 2022

Availability

Academic Institutions and Nonprofits only

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TFAP4_BHLH_5-1

Plasmid

#185054

Purpose

Deletion of BHLH domain of mouse TFAP4 in combination with TFAP4_BHLH_3-2

Depositor

Constance Ciaudo

Article

Schaefer et al EMBO Rep. 2022 Jul 28:e54762. doi: 10.15

Insert

TFAP4_5_1_gRNA (Tcfap4 Mouse)

Use

Lentiviral

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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TFAP4_BHLH_5-2

Plasmid

#185056

Purpose

Deletion of BHLH domain of mouse TFAP4 in combination with TFAP4_BHLH_3-2

Depositor

Constance Ciaudo

Article

Schaefer et al EMBO Rep. 2022 Jul 28:e54762. doi: 10.15

Insert

TFAP4_5_2_gRNA (Tcfap4 Mouse)

Use

Lentiviral

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pN1mcherry-NKD2S31A

Plasmid

#174332

Purpose

encodes human NKD2 S31A mutant cDNA with a C-terminal mCherry fusion.

Depositor

Sonal Srikanth

Article

Wu et al Cell Rep. 2021 Aug 24;36(8):109603. doi:

Insert

Homo sapiens NKD inhibitor of WNT signaling pathway 2 (NKD2 Human)

Tags

mCherry tag

Expression

Mammalian

Mutation

changed Serine 31 to Alanine

Promoter

CMV

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1-NKD2S31A

Plasmid

#174331

Purpose

encodes human NKD2 S31A mutant cDNA with a C-terminal MycHis Tag.

Depositor

Sonal Srikanth

Article

Wu et al Cell Rep. 2021 Aug 24;36(8):109603. doi:

Insert

Homo sapiens NKD inhibitor of WNT signaling pathway 2 (NKD2 Human)

Tags

MycHis

Expression

Mammalian

Mutation

changed Serine 31 to Alanine

Promoter

CMV

Available Since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pBlastR-GMRWhite

Plasmid

#165885

Purpose

Blasticidin resistance vector with attB and eye marker. Contains an Alpha GB cloning entry point for addition of custom assembly uses blue-white screening

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Transgenesis Ready Selection Vector

Use

Synthetic Biology

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pVD2

Plasmid

#165857

Purpose

universal Vector Domesticator vector v2 used in the integration (domestication) of new vector backbone parts in the GB2.0 cloning system. Uses blue-white screening method

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Type

Empty backbone

Use

Synthetic Biology

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pFASTbac-HisGST-PTPIP51(36-470)

Plasmid

#170531

Purpose

Expresses Cleavable HisGST-tagged PTPIP51 (36-470) in Sf9

Depositor

Byung Il Lee

Article

Yeo et al EMBO Rep. 2021 Jun 4;22(6):e51323. doi:

Insert

Protein tyrosine phosphatase interacting protein 51 (RMDN3 )

Tags

GST tag and His tag

Expression

Insect

Promoter

Polyhedrin

Available Since

Aug. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pMB204: UbC-GFP-Tg_SAP30L-WPRE

Plasmid

#168109

Purpose

Lentiviral expression of Zebra Finch SAP30L fused to the C-terminal end of eGFP

Depositor

Erich Jarvis

Article

Biegler et al Sci Rep. 2022 Mar 14;12(1):4369. doi: 10

Insert

SAP30L (SAP30L T. guttata)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Promoter

UbC

Available Since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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pET11a-Z-N-DiB2

Plasmid

#168473

Purpose

N-fragment of the DiB2‐split‐Zip protein. This plasmid needs to be co-transformed with Plasmid 168256: pMRBad-Z-C-wtBlc to obtain DiB2‐split-Zip protein

Depositor

Jens Meiler

Article

Bozhanova et al Sci Rep. 2020 Jul 6;10(1):11049. doi: 10

Insert

N-fragment of DiB2 + Leucine Zipper

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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PWPXLD-EGFR-P667A

Plasmid

#133750

Purpose

EGFR with mutation at proline 667 converted to alanine and tagged with HA cloned into PWPXLD plasmid

Depositor

Chay Kuo

Article

Abdi et al Cell Rep. 2019 Aug 20;28(8):2012-2022.e4

Insert

Mutant P667A EGFR-HA (EGFR Human)

Use

Lentiviral

Tags

HA tag

Expression

Mammalian

Mutation

proline 667 converted to alanine (Please see depo…

Promoter

EF1-alpha

Available Since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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pPuroR-GMRWhite

Plasmid

#165884

Purpose

Puromycin resistance vector with attB and eye marker. Contains an Alpha GB cloning entry point for addition of custom assembly uses blue-white screening

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Transgenesis Ready Selection Vector

Use

Synthetic Biology

Available Since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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