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Showing: 7781 - 7800 of 19284 results

  2. pLCHKO_TK1_HPRT1_Lb_4

    Plasmid
    #155062
    Purpose
    Lentiviral plasmid for expressing U6 driven multiplexed hybrid guide (hg)RNA targeting TK1 using SpCas9 and three intergenic sites & HPRT1 using LbCas12a (CHyMErA system)
    Depositor
    Benjamin Blencowe, Jason Moffat
    Article
    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.
    Insert
    (hg)RNA targeting TK1 using SpCas9 and three intergenic sites & HPRT1 using LbCas12a
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    Aug. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    155062-plasmid-map-sequence-id-308576

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  8. pCAG-memRFP-3xPax7gRNA

    Plasmid
    #224569
    Purpose
    Ubiquitous expression plasmid, CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), three unique Pax7 gRNAs with ribozyme self-cleavage sites, and membrane RFP reporter.
    Depositor
    Erica Hutchins
    Article
    Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026
    Insert
    mRFP1
    Use
    CRISPR
    Tags
    Membrane localization signal
    Mutation
    Codon optimized
    Available Since
    Dec. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    224569-plasmid-map-sequence-id-451497

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  9. pUDP010

    Plasmid
    #101166
    Purpose
    E. coli/S. cerevisiae amdS shuttle vector expressing a ribozyme flanked g-RNA for Cas9 editing targeting the gene SeILV6 in S. pastorianus (HH-gRNASeILV6-HDV)
    Depositor
    Jean-Marc Daran
    Article
    de Vries et al Microb Cell Fact. 2017 Dec 5;16(1):222.
    Insert
    HH-gRNA-HDV targetting SeILV6 in S. pastorianus
    Use
    CRISPR
    Expression
    Yeast
    Promoter
    ScTDH3
    Available Since
    Dec. 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    101166-plasmid-map-sequence-id-199274

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  20. ρ1-EM GABAA receptor in pEZT-BM

    Plasmid
    #213072
    Purpose
    Express human GABA-A rho1 receptor with truncated intracellular domain and superfolder GFP insertion in ICD
    Depositor
    Erik Lindahl
    Article
    Cowgill et al Neuron. 2023 Nov 1;111(21):3450-3464.e5.
    Insert
    Human GABAa rho1 receptor (GABRR1 Human)
    Use
    Bacmam, baculovirus, oocyte expression
    Tags
    Twin-Strep tag and superfolderGFP
    Expression
    Mammalian
    Mutation
    Encodes residues S58–L383 and D451–S479, separate…
    Promoter
    CMV and T7
    Available Since
    Feb. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213072-plasmid-map-sequence-id-423917

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Showing: 7781 - 7800 of 19284 results