We narrowed to 9,984 results for: EPO

1. iCas

   Plasmid

   #84232

   Purpose

   Expression of SpCas9 with 4 ERT2 fusion protein and empty gRNA cassette. The activity of Cas9 can be switched on and off in human cells with 4-hydroxytamoxifen (4-HT)

   Depositor

   Meng How Tan

   Article

   Liu et al Nat Chem Biol. 2016 Sep 12. doi: 10.1038

   Insert

   Cas9
   

   Use

   Tags

   2A-OFP co-expression and ERT2-ERT2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pENTR-ERKKTRClover

   Plasmid

   #59138

   Purpose

   ORF encoding ERK KTR mClover flanked by Gateway sequences

   Depositor

   Markus Covert

   Article

   Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

   Insert

   ERK Kinase Translocation Reporter (MAPK1 Human, Mouse)
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Sept. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. AB-GFP

   Plasmid

   #72203

   Purpose

   screen AB42 variants for aggregation

   Depositor

   Michael Hecht

   Article

   Wurth et al J Mol Biol. 2002 Jun 21;319(5):1279-90.

   Insert

   AB42 (APP Human)
   

   Use

   Tags

   GFP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Dec. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti-PGK-Venus-Akaluc (neo)

   Plasmid

   #124701

   Purpose

   Aka-Luciferase reporter

   Depositor

   Roland Friedel

   Article

   Bozec et al Neurooncol Adv. 2020 Oct 10;2(1):vdaa134

   Insert

   Venus-Akaluciferase
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   hPGK

   Available Since

   Aug. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLL3.7

   Plasmid

   #11795

   Purpose

   3rd generation lentiviral vector that expresses shRNA under the mouse U6 promoter. A CMV-EGFP reporter cassette is included in the vector to monitor expression.

   Depositor

   Luk Parijs

   Article

   Rubinson et al Nat Genet. 2003 Mar . 33(3):401-6.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Cre/Lox, Lentiviral, and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mouse U6

   Available Since

   July 19, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-AID-PcrA M6-UGI-T2A-mTagBFP2

   Plasmid

   #221225

   Purpose

   Express HACE Editor as a fusion of AID cytidine deaminase and an optimized PcrA M6 helicase with mTagBFP2 reporter

   Depositor

   Fei Chen

   Article

   Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

   Insert

   AID-PcrA M6-UGI-T2A-mTagBFP2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. FHRE-Luc

   Plasmid

   #1789

   Purpose

   Reporter plasmid for FOXO3a. Forkhead responsive element with luciferase readout.

   Depositor

   Michael Greenberg

   Article

   Brunet et al Cell 1999 Mar 19;96(6):857-68.

   Insert

   FHRE (FOXO3 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   3 copies of FRE

   Promoter

   Available Since

   Jan. 5, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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8. p2R3a-nosT

   Plasmid

   #71272

   Purpose

   Entry clone containing nosT. Necessary to complete the transcriptional reporters by providing the poly-A tail. For use in plants and compatible with the MultiSite Gateway system

   Depositor

   Ari Pekka Mähönen

   Article

   Siligato et al Plant Physiol. 2015 Dec 7. pii: pp.01246

   Insert

   nopaline synthase terminator
   

   Use

   Gateway

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV.hSynapsin1.OxLight1

   Plasmid

   #169792

   Purpose

   Fluorescent reporter for orexin neuropeptides

   Depositor

   Tommaso Patriarchi

   Article

   Duffet et al Nat Methods. 2022 Feb;19(2):231-241. doi

   Insert

   OxLight1
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   human Synapsin-1

   Available Since

   Feb. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGL3Luc-5XNF-kappaB

    Plasmid

    #185695

    Purpose

    Reporter plasmid with a double-strand oligonucleotides that contain five repeats of NF-kappaB binding consensus sequence (5'-GGGGAATTTCC-3').

    Depositor

    Esther López-Bayghen

    Article

    Hernandez-Melchor et al ASN Neuro. 2022 Jan-Dec;14:1759091422111

    Insert

    NF-kappaB (NFKB1 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    NF-kappaB binding site

    Available Since

    Oct. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. AAV-GfaABC1D-lck-smMyc-4x6T-WPRE

    Plasmid

    #196415

    Purpose

    Astrocytic expression of membrane-targeted Myc spaghetti monster reporter in AAV; astrocyte specificity enhanced with 4x6T miRNA targeting cassette

    Depositor

    Stanley Thomas Carmichael

    Article

    Gleichman et al Nat Commun. 2023 Nov 16;14(1):7426. doi:

    Insert

    Lck-smMyc
    

    Use

    AAV; Astrocyte-selective

    Tags

    Lck

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and GfaABC1D

    Available Since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. CIBN-CAAX

    Plasmid

    #79574

    Purpose

    expression of N-terminal portion of CIB1 with a C-terminal CAAX box from KRras for plasma-membrane targeting

    Depositor

    Olof Idevall-Hagren, Pietro De Camilli

    Article

    Idevall-Hagren et al Proc Natl Acad Sci U S A. 2012 Aug 28;10

    Insert

    CIBN (CIB1 Mustard Weed)
    

    Use

    Tags

    C-terminal CAAX-box

    Expression

    Mammalian

    Mutation

    K93A, R94A, K106A, K107A (please see depositor co…

    Promoter

    CMV

    Available Since

    July 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGL4.10 Dub3

    Plasmid

    #72684

    Purpose

    luciferase reporter with Dub3 promoter

    Depositor

    Domenico Maiorano

    Article

    van der Laan et al PLoS One. 2014 Apr 2;9(4):e93663. doi: 1

    Insert

    Dub3 promoter (Usp17le Mouse)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Dub3

    Available Since

    July 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCSII-EF-miRFP709-hCdt(1/100)

    Plasmid

    #80007

    Purpose

    hCdt(1/100) fused with near-infrared fluorescent protein miRFP709 (G1 phase cell cycle reporter)

    Depositor

    Vladislav Verkhusha

    Article

    Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

    Insert

    hCdt1(1/100) (CDT1 Human)
    

    Use

    Lentiviral

    Tags

    miRFP709

    Expression

    Mutation

    Promoter

    EF-1 alpha

    Available Since

    July 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. Lenti-TargetBC-5xTAPE-1-pegRNA-InsertBC

    Plasmid

    #183790

    Purpose

    A single construct out of the pool of plasmid for lineage recording experiment using DNA Ticker Tape.

    Depositor

    Jay Shendure

    Article

    Choi et al Nature. 2022 Jul 6. pii: 10.1038/s41586-

    Inserts

    P2A-eGFP-TargetBC-5xTAPE-1
    pegRNA-InsertBC
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    G>A in the gRNA scaffold- please see depositor…

    Promoter

    Available Since

    Oct. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti-HRE-dUnaG

    Plasmid

    #124372

    Purpose

    UnaG fluorescent protein reporter for hypoxia-induced factor (HIF) signaling

    Depositor

    Roland Friedel

    Article

    Sattiraju et al Immunity. 2023 Jul 7:S1074-7613(23)00274

    Insert

    HRE-dUnaG
    

    Use

    Lentiviral

    Tags

    PEST-degron and Myc tag

    Expression

    Mutation

    Promoter

    HRE (HIF responsive element 5x + CMV minimal prom…

    Available Since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-AID-BLM-UGI-T2A-mTagBFP2

    Plasmid

    #221222

    Purpose

    Express HACE Editor as a fusion of AID cytidine deaminase and BLM helicase with mTagBFP2 reporter

    Depositor

    Fei Chen

    Article

    Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

    Insert

    AID-BLM-UGI-T2A-mTagBFP2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pmiRGLO Dual-Luciferase-PDAP1-3'UTR-Mut4x

    Plasmid

    #182256

    Purpose

    Human PDAP1 3` UTR region mutated in 4x binding site for miR-150 cloned downstream of luciferase reporter gene

    Depositor

    Silvia Monticelli

    Article

    Chirichella et al PLoS Biol. 2022 Feb 10;20(2):e3001538. d

    Insert

    3' UTR region of PDAP1 gene mutated in 4 binding sites for mir-150
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-TRE-fDIO-GFP-IRES-tTA

    Plasmid

    #118026

    Purpose

    AAV vector for sparse and bright labeling in a cell-type specific manner

    Depositor

    Minmin Luo

    Article

    Lin et al Nat Methods. 2018 Nov 19. pii: 10.1038/s

    Insert

    TRE-fDIO-GFP-IRES-tTA
    

    Use

    AAV

    Tags

    Expression

    Mutation

    See depositor comments below

    Promoter

    TRE

    Available Since

    Dec. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pUbC-FLAG-24xSuntagV4-oxEBFP-AID-baUTR1-24xMS2V5-Wpre

    Plasmid

    #84561

    Purpose

    pSINAPS; single molecule translation reporter containing a FLAG tag, Suntag, oxBFP, AID, beta-actin 3'UTR, and MS2V5 stem-loops

    Depositor

    Robert Singer

    Article

    Wu et al Science. 2016 Jun 17;352(6292):1430-5. d

    Insert

    FLAG tag, Suntag, oxBFP, Auxin-inducible degron, beta-actin 3'UTR, MS2V5 stem-loops
    

    Use

    Lentiviral

    Tags

    Flag, Suntag, oxBFP, AID (auxin-inducible degron)

    Expression

    Mutation

    Promoter

    UbC

    Available Since

    Dec. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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