We narrowed to 13,221 results for: loxP
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Plasmid#216512PurposeIntegrative fluorescent reporter for amino acid biosynthesis sensing pathways, comprising GFP driven by the ARG8 promoter within the Easy clone vector, requires NotI digestion for integration in yeastDepositorInsertPrARG8-yeGFP
UseCre/Lox; IntegrativeExpressionYeastPromoterARG8Available sinceApril 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCfB2197-PrGNP1-mK2-NatMX
Plasmid#216513PurposeIntegrative fluorescent reporter for amino acid uptake sensing pathway, comprising mKate2 driven by the GNP1 promoter within the Easy clone vector, requires NotI digestion for integration in yeastDepositorInsertPrGnp1-mKate2
UseCre/Lox; IntegrativeExpressionYeastPromoterGNP1Available sinceApril 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLenti-MCS-3AID-HA-2A-mCherryBSD
Plasmid#225700PurposeEmpty backbone for lentiviral expression of AID degron tagged POI and mCherry fused BlasticidinRDepositorTypeEmpty backboneUseLentiviral and Synthetic BiologyTagsT2A-mCherryExpressionMammalianPromoterpEF1AAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYFAC-CH2-ubi-Y-pyrG
Plasmid#221131PurposeExpression of heterologous genes under alcohol induction optimised with ubi-Y-pyrG marker. Promoters PalcA-T1-PalcS/M-T2-PaldA expression cassette; unique restriction sites: PacI, NotI, AsiSI, AscI.DepositorTypeEmpty backboneUseSynthetic Biology; Expression in aspergillus and …PromoterPalcA-T1-PalcS/M-T2-PaldA expression cassette (al…Available sinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pACEBac1-PSMA1-PSMA2-PSMA3-PSMA4
Plasmid#217893PurposeInsect cell expression of human 20S CP proteasome subunit alpha type-1 (PSMA1), alpha type-2 (PSMA2), alpha type-3 (PSMA3), and alpha type-4 (PSMA4)DepositorExpressionInsectAvailable sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pACEBac1-PSMB1-PSMB2-PSMB3-PSMB4
Plasmid#217895PurposeInsect cell expression of human 20S CP proteasome subunit beta type-1 (PSMB1), beta type-2 (PSMB2), beta type-3 (PSMB3), and beta type-4 (PSMB4)DepositorExpressionInsectAvailable sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
LiRT_RTctrl
Plasmid#214078PurposeLuminescent reporter system, readthrough control, NMD negative control. Intron present in 5'UTRDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertFirefly luciferase
ExpressionMammalianMutationLinker sequence present between firefly luciferas…Available sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2-TetO-hSOX2
Plasmid#206388PurposeExpresses human SOX2 in mammalian cells, for lentivirus generation.DepositorInsertSOX2 (SOX2 Human)
UseLentiviralAvailable sinceOct. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2-TetO-GFP-mSox2
Plasmid#206374PurposeExpresses EGFP fused mouse SOX2 in mammalian cells, for lentivirus generation.DepositorPublicationAvailable sinceOct. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDropIn-T2A-split-intein-Gal4[1-20]-N-int
Plasmid#199195PurposeInsert to create split-intein Gal4[N-int] knock-in vectors via Drop-In cloning, released via BsmBI digestDepositorInsertT2A-split-intein-Gal4[1-20]-N-int, 3xP3-dsRed
ExpressionInsectAvailable sinceAug. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-sCAG-GluClβ.CreON
Plasmid#196996PurposeCre recombinase dependent expression of GluClv2.0 beta subunit. GluClβ contains a YFP tag. When co-expressed with GluClv2.0 alpha subunit, agonist (Ivermectin) induces neuronal silencing.DepositorInsertGluClβ -YFP
UseAAV and Cre/LoxTagsYFPPromotershort CAGAvailable sinceJune 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEasyG1_zeo
Plasmid#184910PurposeTemplate to generate via PCR a single gRNA for expression in S. cerevisiaeDepositorInsertgRNA scaffold
UseCRISPRExpressionBacterialAvailable sinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMK438 (Stag-3FLAG-P2A-Hygro)
Plasmid#168233PurposeC-terminal tagging with Stag-3FLAGDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertStag-3FLAG-P2A-Hygro
UseCRISPRExpressionMammalianAvailable sinceOct. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSEM415 - mosTI unc-119 Pmex-5 - gfp(nls) - tbb-2
Plasmid#182358PurposePositive control for mosTI(unc-119). unc-119 rescue and germline nuclear GFP expression after mosTI insertion.DepositorInsertGFP
ExpressionWormPromoterPmex-5Available sinceMay 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOmnibac_ZZ_linker_ybbR_MAP7
Plasmid#159718PurposeExpresses MAP7 in Sf9 cells with a ZZ affinity tag and a ybbR tagDepositorInsertMAP7 (Microtubule Associated Protein 7) (MAP7 Human)
TagsZZ affinity tag, ybbR tagExpressionInsectAvailable sinceJan. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYL236
Plasmid#173184PurposeCRISPR donor plasmid for making the WT EZH2 control cell line. It contains a puromycin resistance gene and an mCherry geneDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertEZH2 (EZH2 Human)
UseCRISPRExpressionMammalianAvailable sinceAug. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pAAV.hSynap-FLEX.SF-Venus-iGluSnFR.S72A
Plasmid#106185PurposeWeak affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **DepositorHas serviceAAV1InsertSF-Venus-iGluSnFR.S72A
UseAAVMutationSFGFP: T203Y, F46L, T65G, S72A GltI: S72APromoterhSynapsinAvailable sinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti_C_Myc_DDK_IRES_Puro_UFD1L
Plasmid#106118PurposeLentiviral expression of UFD1LDepositorAvailable sinceFeb. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
EKAR2G_design1_mTFP_159_Venus_173
Plasmid#39825DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertErk activity reporter
TagsHis-MycExpressionBacterial, Insect, and Mamm…PromoterCMVAvailable sinceJuly 5, 2013AvailabilityAcademic Institutions and Nonprofits only