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TMK1 X035_pECIA14

Plasmid

#114871

Purpose

Prey vector TMK1 X035_pECIA14 should be used with bait vector TMK1 X035_pECIA2.

Depositor

Youssef Belkhadir

Publication

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT1G66150 (TMK1 Mustard Weed)

Expression

Insect

Promoter

Metallothionein (Copper-Inducible)

Available since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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pZac2.1-GfaABC1D-mCherry-hPMCA2x/b

Plasmid

#111569

Purpose

Expresses a plasma membrane calcium pump hPMCA2x/b

Depositor

Baljit Khakh

Publication

Yu et al Neuron. 2018 Aug 27. pii: S0896-6273(18)

Insert

Plasma membrane calcium pump 2, variant x/b (ATP2B2 Human)

Tags

mCherry

Available since

Nov. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA5-MIS12-LAP-BUB1

Plasmid

#115904

Purpose

Used for expression of siRNA-resistant MIS12-BUB1 from the FRT site of FlpIn cells upon addition of doxycyclin.

Depositor

Geert Kops

Publication

Vleugel et al J Cell Sci. 2015 Aug 15;128(16):2975-82.

Insert

MIS12-BUB1 (MIS12 Human)

Tags

EYFP

Expression

Mammalian

Mutation

BUB1 fused to MIS12

Available since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pRS406 ATG9-GFP S122D

Plasmid

#114667

Purpose

Expresses yeast ATG9 (S122D) under the endogenous promoter with a GFP C-terminal tag

Depositor

Daniel Klionsky

Publication

Feng et al Autophagy. 2016;12(4):648-58. doi: 10.10

Insert

ATG9

Tags

GFP

Expression

Yeast

Mutation

changed serine 122 to aspartic acid

Promoter

ATG9

Available since

Sept. 13, 2018

Availability

Academic Institutions and Nonprofits only

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MIS12-FRB-FLAG

Plasmid

#114040

Purpose

Kinetochore localised FRB tagged with FLAG

Depositor

Geert Kops

Publication

Kuijt et al Chromosoma. 2014 Oct;123(5):471-80. doi:

Insert

MIS12 (MIS12 Human)

Tags

FRB-FLAG-tagRFP

Expression

Mammalian

Promoter

CMV

Available since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

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1184_pAAV-U6-ApoB-gRNA2-CB-EmGFP

Plasmid

#89062

Purpose

AAV-gRNA targeting the murine Apob gene

Depositor

William Lagor

Publication

Jarrett et al Sci Rep. 2017 Mar 16;7:44624. doi: 10.10

Insert

EmGFP

Use

AAV and CRISPR

Expression

Mammalian

Promoter

Chicken beta actin with partial CMV enhancer

Available since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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pFBD-Akt1(K14A)

Plasmid

#86562

Purpose

Expresses human full-length Akt1(K14A) mutant

Depositor

Thomas Leonard, Ivan Yudushkin

Publication

Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

Insert

Akt1 (AKT1 Human)

Tags

10xHis

Expression

Insect

Mutation

K14A mutation in the PH domain

Available since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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Human NCKX3 (pcDNA3.1+)

Plasmid

#75205

Purpose

Mammalian expression of SLC24A3

Depositor

Jonathan Lytton

Publication

Kraev et al J Biol Chem. 2001 Jun 22;276(25):23161-7

Insert

SLC24A3 (SLC24A3 Human)

Expression

Mammalian

Promoter

CMV

Available since

June 21, 2016

Availability

Academic Institutions and Nonprofits only

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pTAtUbq3 (GB0219)

Plasmid

#68192

Purpose

Provides the Arabidopsis thaliana polyubiquitin 3 terminator as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Publication

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

AtUbq3 terminator

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available since

March 16, 2016

Availability

Academic Institutions and Nonprofits only

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pPAct2noUTR (GB0192)

Plasmid

#68177

Purpose

Provides the Arabidopsis thaliana actin2 gene promoter (without 5' UTR) as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Publication

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

AtAct2 promoter (no UTR)

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available since

March 16, 2016

Availability

Academic Institutions and Nonprofits only

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FCIV1-GR S155A

Plasmid

#72702

Purpose

Lentiviral expression of GR Phospho-deficient mutant (2nd generation)

Depositor

Freddy Jeanneteau

Publication

Arango-Lievano et al Proc Natl Acad Sci U S A. 2015 Nov 16. p

Insert

GR (Nr3c1 Rat)

Use

Lentiviral

Tags

IRES-Venus

Expression

Mammalian

Mutation

S155A

Promoter

ubiquitin

Available since

Feb. 9, 2016

Availability

Academic Institutions and Nonprofits only
pCellFree_G03 SMAD2

Plasmid

#67095

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Publication

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

SMAD2 (SMAD2 Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
NET37 pmRFP-N2 (450)

Plasmid

#61980

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Publication

Malik et al Cell Mol Life Sci. 2010 Apr;67(8):1353-6

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

NET37 (KIAA1161 Human)

Tags

mRFP

Expression

Mammalian

Promoter

CMV

Available since

Aug. 17, 2015

Availability

Academic Institutions and Nonprofits only
pCMB-TMEg

Plasmid

#61209

Purpose

Fluorescent reporter for plasma membrane and trunk peri-arbuscular membrane MtBCPsp-GFP-BCP expressed under MtBCP1 promoter

Depositor

Maria Harrison

Publication

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

MtBCPsp-GFP-BCP

Tags

EGFP

Expression

Plant

Promoter

MtBCP1

Available since

April 10, 2015

Availability

Academic Institutions and Nonprofits only

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MYLK4

Plasmid

#39175

Purpose

Baculovirus expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

MYLK4 (MYLK4 Human)

Use

Baculovirus expression

Tags

His Tev

Mutation

N371S, codon-optimized

Available since

March 18, 2013

Availability

Academic Institutions and Nonprofits only
XE3 Xwnt-5A Full Length

Plasmid

#16875

Depositor

Randall Moon

Publication

Moon et al Development. 1993 Sep. 119(1):97-111.

Insert

Xwnt-5A (wnt5a.L Frog)

Use

Xenopus expression

Available since

Sept. 6, 2012

Availability

Academic Institutions and Nonprofits only
TAL2398

Plasmid

#36801

Depositor

Keith Joung

Publication

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

MYCL1-TALEN_Left (Mycl Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
TAL2399

Plasmid

#36802

Depositor

Keith Joung

Publication

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

MYCL1-TALEN_Right (Mycl Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
pEMS1231

Plasmid

#29117

Purpose

Expression of the eGFP reporter gene was not detected in the brain and eye.

Depositor

Elizabeth Simpson

Publication

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Insert

Ple205 (SLC7A5 Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

EGFP-NLS

Available since

Oct. 3, 2011

Availability

Academic Institutions and Nonprofits only

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CCD+BATS

Plasmid

#30499

Depositor

Qing Zhong

Publication

Fan et al Proc Natl Acad Sci U S A. 2011 May 10;10

Insert

Barkor (ATG14 Human)

Tags

3xFlag and hrGFP

Expression

Mammalian

Mutation

71-180aa (CCD) + 413-492aa (BATS)

Available since

July 11, 2011

Availability

Academic Institutions and Nonprofits only