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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 18,006 results for: puro

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Showing: 7901 - 7920 of 18006 results

  5. piggyFlex

    Plasmid
    #218234
    Purpose
    A piggyBac transposon-based gRNA expression vector, to allow for genomic integration and stable expression of gRNAs. Contains both antibiotic (puromycin) and fluorophore (GFP) markers.
    Depositor
    Jay Shendure
    Article
    Chardon et al Nat Commun. 2024 Sep 18;15(1):8209. doi:
    Type
    Empty backbone
    Use
    CRISPR
    Available Since
    May 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    218234-plasmid-map-sequence-id-432525

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  6. HSC1-GiP

    Plasmid
    #58254
    Purpose
    This self-inactivated retroviral vector has an internal human EF1alpha promoter driving the expression of a bicistronic EGFP ires Puromycin cassette
    Depositor
    James Ellis
    Article
    Rival-Gervier et al Mol Ther. 2013 Aug;21(8):1536-50. doi: 1
    Insert
    Enhanced Green Fluorescence Protein, Puromycin resistance gene
    Use
    Retroviral
    Expression
    Mammalian
    Promoter
    Human EF1-alpha
    Available Since
    Oct. 10, 2014
    Availability
    Academic Institutions and Nonprofits only
    58254-plasmid-map-sequence-id-321491

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  8. pMP472

    Plasmid
    #134997
    Purpose
    Interspersed Reporter. Fluorescent reporter to be used with m6A readers and writers. (8xZF binding sites + 14xGATC sites)-pMinCMV-GFPd2-RbGpA pGK-PuroR-T2A-mCh-BGHpA AAVS1 donor
    Depositor
    Ahmad Khalil
    Article
    Park et al Cell. 2019 Jan 10;176(1-2):227-238.e20.
    Insert
    Inters. (8XZFBS 14XGATC)-pMinCMV-GFPd2-RbGpA pGK-PuroR-T2A-mCh-BGHpA AAVS1 donor
    Use
    AAV and Synthetic Biology
    Expression
    Mammalian
    Available Since
    March 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    134997-plasmid-map-sequence-id-267923

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  9. pMP498

    Plasmid
    #134998
    Purpose
    Clustered Reporter. Fluorescent reporter to be used with m6A readers and writers. (5xZF binding sites + 63xGATC sites)-pMinCMV-GFPd2-RbGpA pGK-PuroR-T2A-mCh-BGHpA AAVS1 donor
    Depositor
    Ahmad Khalil
    Article
    Park et al Cell. 2019 Jan 10;176(1-2):227-238.e20.
    Insert
    Clust. (5XZFBS 63XGATC)-pMinCMV-GFPd2-RbGpA pGK-PuroR-T2A-mCh-BGHpA AAVS1 donor
    Use
    AAV and Synthetic Biology
    Expression
    Mammalian
    Available Since
    March 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    134998-plasmid-map-sequence-id-269998

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  11. pHDR-PMG (pXK-1650)

    Plasmid
    #208858
    Purpose
    HDR-base selectable traffic light reporter (TLR) for helping to screen HDR-editing positive cells. Key Construct: iPuroR*-CMV-PuroR.L-mCherry-PuroR.R-GFP.
    Depositor
    Kun Xu
    Article
    Lyu et al Int J Biol Macromol. 2023 Jul 15;243:124
    Type
    Empty backbone
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Dec. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    208858-plasmid-map-sequence-id-417249

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  12. Cas9-sgRNA-A

    Plasmid
    #149369
    Purpose
    Expresses SpCas9 and sgRNA targeting the lenti-CDDR reporter
    Depositor
    Tarek Abbas
    Article
    Eki et al Nucleic Acids Res. 2020 Oct 17. pii: 592
    Inserts
    Cas9
    sgRNA-A
    Puromycin resistance
    Use
    CRISPR
    Tags
    3xFLAG, Nucleoplasmin NLS, and SV40 NLS
    Expression
    Mammalian
    Promoter
    CBh, PGK, and U6
    Available Since
    Nov. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    149369-plasmid-map-sequence-id-313973

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  13. Cas9-sgRNA-A+B

    Plasmid
    #149370
    Purpose
    Expresses SpCas9 and two sgRNA targeting the lenti-CDDR reporter at two distal sites
    Depositor
    Tarek Abbas
    Article
    Eki et al Nucleic Acids Res. 2020 Oct 17. pii: 592
    Inserts
    Cas9
    sgRNA-A
    sgRNA-B
    Puromycin resistance
    Use
    CRISPR
    Tags
    3xFLAG, Nucleoplasmin NLS, and SV40 NLS
    Expression
    Mammalian
    Promoter
    CBh, PGK, and U6
    Available Since
    Oct. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    149370-plasmid-map-sequence-id-315474

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  18. hZMPSTE24-3FLAG_pQCXIP

    Plasmid
    #89759
    Purpose
    Human ZMPSTE24 mammalian expression vector. Retroviral bicistronic puromycin vector.
    Depositor
    Martin Dorf
    Article
    Fu et al J Exp Med. 2017 Feb 28. pii: jem.2016127
    Insert
    Homo sapiens zinc metallopeptidase STE24 (ZMPSTE24) (ZMPSTE24 Human)
    Use
    Retroviral; Bicistronic expression: puromycin re…
    Tags
    9bp spacer (NotI site) - 3xFLAG epitopes - Stop c…
    Expression
    Mammalian
    Mutation
    Y253H in ZMPSTE24 (see depositor comments below)
    Promoter
    CMV
    Available Since
    June 8, 2017
    Availability
    Academic Institutions and Nonprofits only
    89759-plasmid-map-sequence-id-180605

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Showing: 7901 - 7920 of 18006 results