We narrowed to 18,167 results for: STO;

1. pCHC022 (∆phcA)

   Plasmid

   #226200

   Purpose

   Electroporation knockout plasmid (pK18sB) to delete the C. necator LysR-type transcriptional regulator PhcA.

   Depositor

   Christopher Johnson

   Article

   Calvey et al Metab Eng. 2023 Jan;75:78-90. doi: 10.10

   Insert

   Homology arms for PhcA
   

   Use

   Synthetic Biology

   Available Since

   Nov. 18, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pCHC004 (∆phaCAB)

   Plasmid

   #226198

   Purpose

   Electroporation knockout plasmid (pK18sB) to delete the C. necator phaCAB operon, to prevent accumulation of PHB.

   Depositor

   Christopher Johnson

   Article

   Calvey et al Metab Eng. 2023 Jan;75:78-90. doi: 10.10

   Insert

   Homology arms for phaCAB
   

   Use

   Synthetic Biology

   Available Since

   Nov. 15, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pCHC027 (∆pemK)

   Plasmid

   #226204

   Purpose

   Conjugation knockout plasmid (pK18msB) to delete the C. necator addiction system toxin PemK, to enable deletion of the pHG1 megaplasmid.

   Depositor

   Christopher Johnson

   Article

   Calvey et al Metab Eng. 2023 Jan;75:78-90. doi: 10.10

   Insert

   Upstream/downstream homology arms for addiction system toxinPemK.
   

   Use

   Synthetic Biology

   Available Since

   Nov. 14, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. 8_T7-PH-mScarlet3

   Plasmid

   #225935

   Purpose

   In vitro synthesis of mRNA for fluorescent protein mScarlet3 tagged with the PH membrane localisation signal

   Depositor

   Michalis Averof

   Article

   Karapidaki et al bioRxiv 2024.11.12.623055

   Insert

   PH-mScarlet3
   

   Use

   In vitro transcription

   Promoter

   T7

   Available Since

   Nov. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLIX401 APE1 SO

   Plasmid

   #227192

   Purpose

   Inducible lentiviral expression of SO (Slow Off) APE1

   Depositor

   David Pellman

   Article

   Tang et al Nature. 2022 Jun;606(7916):930-936. doi:

   Insert

   apurinic/apyrimidinic endonuclease APE1 (APEX1 Human)
   

   Expression

   Bacterial and Mammalian

   Mutation

   D283A D308A

   Available Since

   Nov. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. FUS-miRFP670-TRF1

   Plasmid

   #227378

   Purpose

   Adhesion module, creates 'seed' at telomere

   Depositor

   Clifford Brangwynne

   Article

   Strom et al Cell. 2024 Aug 14:S0092-8674(24)00828-6.

   Insert

   Telomeric FUS IDR, WT (TERF1 Human)
   

   Use

   Lentiviral

   Available Since

   Oct. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. iLID-miRFP670-TRF1

   Plasmid

   #227385

   Purpose

   Adhesion module, creates 'seed' at telomere

   Depositor

   Clifford Brangwynne

   Article

   Strom et al Cell. 2024 Aug 14:S0092-8674(24)00828-6.

   Insert

   Telomeric iLID (TERF1 Human)
   

   Use

   Lentiviral

   Available Since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1(+) M-Ras(G22C)

   Plasmid

   #224288

   Purpose

   Cell transfection and expression of M-Ras (G22C)

   Depositor

   Kevan Shokat

   Article

   Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

   Insert

   M-Ras (MRAS Human)
   

   Expression

   Mammalian

   Mutation

   G22C

   Available Since

   Sept. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1(+) Rheb(R15C)

   Plasmid

   #224290

   Purpose

   Cell transfection and expression of Rheb (R15C)

   Depositor

   Kevan Shokat

   Article

   Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

   Insert

   Rheb (RHEB Human)
   

   Expression

   Mammalian

   Mutation

   R15C

   Available Since

   Sept. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1(+) R-Ras1(G38C)

    Plasmid

    #224286

    Purpose

    Cell transfection and expression of R-Ras1 (G38C)

    Depositor

    Kevan Shokat

    Article

    Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

    Insert

    R-Ras1 (RRAS Human)
    

    Expression

    Mammalian

    Mutation

    G38C

    Available Since

    Sept. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1(+) EGFP-RalA(G23C)

    Plasmid

    #224275

    Purpose

    cell transfection and expreswsion of RalA (G23C)

    Depositor

    Kevan Shokat

    Article

    Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

    Insert

    RalA (RALA Human)
    

    Expression

    Mammalian

    Mutation

    G23C

    Available Since

    Sept. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pT7-IVT_ACC-msfGFP[r5M]_ACC-mEBFP2

    Plasmid

    #221082

    Purpose

    Plasmid for producing DNA Templates for in vitro transcription. Produces msfGFP[r5M] and mEBFP2 from IVT mRNA.

    Depositor

    Mikhail Shapiro

    Article

    Duan et al Cell Syst. 2024 Jul 17;15(7):597-609.e4.

    Insert

    ACC-msfGFP[r5M]_ACC-mEBFP2
    

    Use

    Synthetic Biology

    Promoter

    CMV, T7

    Available Since

    Sept. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. LentiCRISPRv2 EGFP sgRNA4

    Plasmid

    #164934

    Purpose

    Expresses EGFP sgRNA4 in mammalian cells

    Depositor

    Ji Luo

    Article

    Yuen et al Nucleic Acids Res. 2017 Nov 16;45(20):12

    Insert

    sgRNA4 targeting Enhanced green fluorescent protein (verified for knockout)
    

    Use

    Lentiviral

    Promoter

    EFS promoter

    Available Since

    July 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. LentiCRISPRv2 NT sgRNA5

    Plasmid

    #164930

    Purpose

    Expresses Non-targeting sgRNA5 control in mammalian cells

    Depositor

    Ji Luo

    Article

    Yuen et al Nucleic Acids Res. 2017 Nov 16;45(20):12

    Insert

    Non-targeting sgRNA5 (verified for knockout)
    

    Use

    Lentiviral

    Promoter

    EFS promoter

    Available Since

    July 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGex-6p2_ATRX_K1033R

    Plasmid

    #221378

    Purpose

    Expresses ATRX_K1033R in bacterial cells

    Depositor

    Albert Jeltsch

    Article

    Weirich et al Commun Biol. 2024 Jun 8;7(1):707. doi: 1

    Insert

    Transcriptional regulator ATRX (ATRX Human)
    

    Expression

    Bacterial

    Mutation

    Changed lysine 1033 to arginine

    Promoter

    tac

    Available Since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGex-6p2_FANCM_K819R

    Plasmid

    #221380

    Purpose

    Expresses FANCM_K819R in bacterial cells

    Depositor

    Albert Jeltsch

    Article

    Weirich et al Commun Biol. 2024 Jun 8;7(1):707. doi: 1

    Insert

    Fanconi anemia group M protein (FANCM Human)
    

    Expression

    Bacterial

    Mutation

    Changed lysine 819 to arginine

    Promoter

    tac

    Available Since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEYFP_C1_ATRX

    Plasmid

    #221383

    Purpose

    Expresses ATRX in mammalian cells

    Depositor

    Albert Jeltsch

    Article

    Weirich et al Commun Biol. 2024 Jun 8;7(1):707. doi: 1

    Insert

    Transcriptional regulator ATRX (ATRX Human)
    

    Expression

    Mammalian

    Mutation

    K536N (Please see depositor comments)

    Promoter

    CMV

    Available Since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCPH2A.Z-GS3

    Plasmid

    #204758

    Purpose

    Expression of Cas9 and human H2A.Z

    Depositor

    Yuichiro Miyaoka

    Article

    Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

    Insert

    H2AZ1 (H2AZ1 Human)
    

    Expression

    Mammalian

    Available Since

    June 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. TDP-43 mRuby K84ONBK

    Plasmid

    #216226

    Purpose

    TDP-43 with a C-terminal mRuby tag and Lysine 84 in the NLS mutated to a TAG stop codon for amber codon suppression.

    Depositor

    Jennifer Lee

    Article

    Shadish et al Biophys Chem. 2024 Jan 23;307:107191. do

    Insert

    Transactive response DNA binding protein of 43 kDa (TARDBP Human)
    

    Expression

    Mammalian

    Mutation

    Lysine 84 mutated to a TAG stop codon

    Promoter

    CMV Promoter

    Available Since

    April 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCL1315

    Plasmid

    #217957

    Purpose

    Expression of human H3.1 carrying a triple point mutation to disrupt folding with histone H4. Expression in mammalian cells driven by the CMV promoter.

    Depositor

    Andrew Bowman

    Article

    Pardal et al Elife. 2022 Sep 6;11:e81755. doi: 10.755

    Insert

    Histone H3.1 (H3C1 Human)
    

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    F84A, L92A, Y99A

    Promoter

    CMV

    Available Since

    April 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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