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pMT Draper-mCherry

Plasmid

#126667

Purpose

Inducible expression of Draper-mCherry in S2 cells

Depositor

Ron Vale

Article

Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

Insert

Draper mCherry

Expression

Insect

Mutation

C813Y- see depositor comments below

Available Since

June 8, 2021

Availability

Academic Institutions and Nonprofits only

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pTZ U6+27

Plasmid

#25713

Purpose

for nuclear expression of small RNAs in mammalian cells

Depositor

David Engelke

Article

Good et al Gene Ther. 1997 Jan . 4(1):45-54.

Insert

U6 snRNP (RNU6-1 Human)

Expression

Mammalian

Available Since

June 24, 2010

Availability

Academic Institutions and Nonprofits only
pCG-B-SpyTag-C

Plasmid

#87373

Purpose

An ORF part-containing vector harbouring the SpyTag sequence with 4 bp overhangs specific for the ‘upstream SpyPart’ position within an ORF assembly

Depositor

Tom Ellis

Article

Gilbert et al ACS Synth Biol. 2017 Mar 7. doi: 10.1021

Insert

SpyTag

Use

Synthetic Biology

Available Since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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pQE-WT-KIF-R5-KIKR

Plasmid

#63624

Purpose

KIF-R5-KIKR RNAP mutant

Depositor

Andrew Ellington

Article

Meyer et al ACS Synth Biol. 2014 Oct 7.

Insert

KIF-R5-KIKR RNAP

Mutation

Q744K, L749I, M750K, Q754S, R756N, I761V, H772R

Promoter

T5/Lac

Available Since

April 15, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pET15b-eIF4E

Plasmid

#37228

Depositor

Jon Lorsch

Article

Mitchell et al Mol Cell. 2010 Sep 24;39(6):950-62.

Insert

eIF4E

Expression

Bacterial

Promoter

T7

Available Since

July 16, 2012

Availability

Academic Institutions and Nonprofits only
pTH07

Plasmid

#70063

Purpose

artificial transcription template "distal pause"

Depositor

Jeff Gelles

Article

Harden et al Proc Natl Acad Sci U S A. 2016 Jan 5. pi

Insert

lambda PR' - repeat cassette - E. coli rpoB with inserted pause - lambda TR'

Available Since

Jan. 7, 2016

Availability

Academic Institutions and Nonprofits only
pTH09

Plasmid

#70064

Purpose

artificial transcription template "terminator insertion"

Depositor

Jeff Gelles

Article

Harden et al Proc Natl Acad Sci U S A. 2016 Jan 5. pi

Insert

lambda PR' - lambda TR2 - repeat cassette - E. coli rpoB - lambda TR'

Available Since

Jan. 7, 2016

Availability

Academic Institutions and Nonprofits only
pNHA9

Plasmid

#223642

Purpose

pUC18 plasmid with rpsM promoter

Depositor

François-Xavier Campbell-Valois

Article

Haidar-Ahmad et al mSphere. 2024 Nov 21;9(11):e0055324. doi

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Oct. 28, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pUPD2-LRR-NEL (B4-B5)

Plasmid

#211859

Purpose

CDS for E3-DART ligase in domestication vector

Depositor

Marcela Rojas-Pierce

Article

Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.

Insert

LRR-NEL

Use

Synthetic Biology

Available Since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

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pFH2.18_TagBFP

Plasmid

#205533

Purpose

Express TagBFP

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

TagBFP

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available Since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pWSK29-wzzpHS2

Plasmid

#172971

Purpose

pWSK29 with S. flexneri wzzpHS2 gene

Depositor

Renato Morona

Article

Murray et al J Bacteriol. 2006 Apr;188(7):2735-9. doi

Insert

wzzPHS2 gene from Shigella flexneri

Expression

Bacterial

Available Since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pCAFNF-EGFP

Plasmid

#163609

Purpose

Expresses EGFP Flp-dependently in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Egfp

Expression

Mammalian

Available Since

Aug. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pWSK29-wzzBSF

Plasmid

#172967

Purpose

pWSK29 with S. flexneri 2a wzzBSF gene

Depositor

Renato Morona

Article

Murray et al J Bacteriol. 2006 Apr;188(7):2735-9. doi

Insert

wzzB gene from Shigella flexneri

Expression

Bacterial

Available Since

Aug. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pRMCD122

Plasmid

#172963

Purpose

pRMCD77 encoding Wzz mutant I35C/M32T

Depositor

Renato Morona

Article

Daniels et al Mol Microbiol. 1999 Oct;34(1):181-94. do

Insert

Shigella flexneri wzz I35C/M32T mutant gene

Expression

Bacterial

Available Since

Aug. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pRMCD112

Plasmid

#172958

Purpose

pRMCD77 encoding Wzz mutant G305A/G309A

Depositor

Renato Morona

Article

Daniels et al Mol Microbiol. 1999 Oct;34(1):181-94. do

Insert

Shigella flexneri wzz G305A/G309A mutant gene

Expression

Bacterial

Available Since

Aug. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-luc-miR-1

Plasmid

#171556

Purpose

a positive control reporter with 3' UTR sequences perfectly complementary to miR-1

Depositor

Yan Zeng

Article

Li et al Cells. 2019 Jul 30;8(8). pii: cells80807

Insert

a sequences perfectly complementary to miR-1

Expression

Mammalian

Available Since

Aug. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pDL128

Plasmid

#43912

Depositor

Stephen Elledge

Article

Lee et al Mol Cell. 2008 Oct 10;32(1):70-80. doi:

Insert

DIF1 (DIF1 Budding Yeast)

Expression

Yeast

Mutation

SML domain mutated PPMLINQRT->AAAAAAAA, S59G, …

Available Since

Aug. 15, 2013

Availability

Academic Institutions and Nonprofits only
pDL127

Plasmid

#43911

Depositor

Stephen Elledge

Article

Lee et al Mol Cell. 2008 Oct 10;32(1):70-80. doi:

Insert

DIF1 (DIF1 Budding Yeast)

Expression

Yeast

Mutation

HUG domain mutated VGMRIRQ->AAAAAAA, S59, Q66H

Available Since

Aug. 15, 2013

Availability

Academic Institutions and Nonprofits only
pMT Ptp69D-GFP

Plasmid

#126663

Purpose

Inducible expression of Ptp69D-GFP in S2 cells

Depositor

Ron Vale

Article

Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

Insert

Ptp69D

Expression

Insect

Mutation

A53S, K105N, and A98T- please see depositor comme…

Availability

Academic Institutions and Nonprofits only

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pET His10-Draper INT

Plasmid

#126670

Purpose

Expression of His10-Draper intracellular domain for purification from E. coli

Depositor

Ron Vale

Article

Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

Insert

Draper cytoplasmic domain

Expression

Bacterial

Availability

Academic Institutions and Nonprofits only

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