Addgene

We narrowed to 31,294 results for: Eng

Showing: 91 - 120 of 31294 results

Results per page:

Sort results by:

full length png in pGEX4t

Plasmid

#112974

Purpose

For protein expression and purification of full-length Drosophila png

Depositor

Terry Orr-Weaver

Article

Hara et al Elife. 2017 May 30;6. doi: 10.7554/eLife

Insert

png (png Fly)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-28a RUVBL2 Full Length

Plasmid

#159142

Purpose

Expresssion and protein purification of full length RUVBL2

Depositor

Eric Erquan Zhang

Article

Ju et al Sci Transl Med. 2020 May 6;12(542). pii:

Insert

RUVBL2 (RUVBL2 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 14, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-N1 Full length Importin beta

Plasmid

#106941

Purpose

Transient expression of GFP-tagged Importin beta in mammalian cells (CMV promoter)

Depositor

Patrizia Lavia

Article

Ciciarello et al J Cell Sci. 2004 Dec 15. 117(Pt 26):6511

Insert

human Importin beta-1 (KPNB1) (KPNB1 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
p5068 pGEX-6P-1 Brd4 full-length

Plasmid

#14447

Depositor

Peter Howley

Article

You et al Cell. 2004 Apr 30. 117(3):349-60.

Insert

Brd4 (BRD4 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

March 2, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pAPtag5-IgK-NCAN-Full length

Plasmid

#219812

Purpose

Expresses His-tagged NCAN-Full length to produce recombinant protein

Depositor

Cagla Eroglu

Article

Irala et al Neuron. 2024 Mar 27:S0896-6273(24)00161-

Insert

IgK (Igk Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 17, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2 Notch1 Full Length-6MT

Plasmid

#41728

Depositor

Raphael Kopan

Article

Schroeter et al Nature. 1998 May 28;393(6683):382-6.

Insert

Notch-1 Full Length (Notch1 Mouse)

Use

Tags

6xMyc

Expression

Mammalian

Mutation

L1997V; Contains murine Notch-1 protein up to ami…

Promoter

Available since

Dec. 18, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pOPINK-OTUB1 (full-length, aa 1-271)

Plasmid

#61420

Purpose

Expresses human OTUB1 (full-length) in E. coli.

Depositor

David Komander

Article

Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

Insert

OTUB1 (Ubiquitin thioesterase OTUB1) (OTUB1 Human)

Use

Tags

His6-GST-3C

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pRK5-mEGFP-HMGB1-WT-Full-length

Plasmid

#194548

Purpose

Mammalian Expression of mEGFP-HMGB1 WT Fusion Protein

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

HMGB1 (HMGB1 Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pOPINB-HOIL-1 full-length (1–510)

Plasmid

#193858

Purpose

Expression of human His-tagged HOIL-1 (RBCK1) full-length (1–510) codon optimized for E. coli

Depositor

Bernhard Lechtenberg

Article

Wang et al Nat Commun. 2023 Jan 11;14(1):168. doi:

Insert

HOIL-1 (RBCK1 Human)

Use

Tags

3C protease cleavage site and 6x-His tag

Expression

Bacterial

Mutation

Codon optimised for expression in E. coli

Promoter

T7

Available since

Jan. 12, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBacMam2-DiEx-LIC-N-flag_huntingtin_full-length_Q23

Plasmid

#111723

Purpose

Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells

Depositor

Cheryl Arrowsmith

Article

Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70

Insert

HTT (HTT Human)

Use

Baculovirus expression

Tags

FLAG

Expression

Mammalian

Mutation

Q23 (polyQ repeat)

Promoter

CMV and P10

Available since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
p4947 pcDNA4c hBrd4 full-length

Plasmid

#14441

Depositor

Peter Howley

Article

You et al Cell. 2004 Apr 30. 117(3):349-60.

Insert

Brd4 (BRD4 Human)

Use

Tags

6xHis, T7 tag, and Xpress epitope

Expression

Mammalian

Mutation

Promoter

Available since

March 2, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pET 28 full-length human PARP1

Plasmid

#192635

Purpose

Expresses human PARP1 with an N-terminal His-tag

Depositor

John Pascal

Article

Langelier et al J Biol Chem. 2008 Feb 15;283(7):4105-14.

Insert

Human PARP1 (PARP1 Human)

Use

Tags

histidine-tag

Expression

Bacterial

Mutation

Polymorphism A762

Promoter

Available since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q145

Plasmid

#111731

Purpose

Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells

Depositor

Cheryl Arrowsmith

Article

Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70

Insert

HTT (HTT Human)

Use

Baculovirus expression

Tags

FLAG

Expression

Mammalian

Mutation

Q145 (polyQ repeat)

Promoter

CMV and P10

Available since

Nov. 1, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
Psicheck2 PTPRN2 full length 3'UTR

Plasmid

#26994

Depositor

Joan Massague

Article

Tavazoie et al Nature. 2008 Jan 10. 451(7175):147-52.

Insert

PTPRN2 Full length 3' UTR (PTPRN2 Human)

Use

Luciferase

Tags

Renilla Luciferase

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 11, 2011

Availability

Academic Institutions and Nonprofits only
pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q23

Plasmid

#111726

Purpose

Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells

Depositor

Cheryl Arrowsmith

Article

Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70

Insert

HTT (HTT Human)

Use

Baculovirus expression

Tags

FLAG

Expression

Mammalian

Mutation

Q23 (polyQ repeat)

Promoter

CMV and P10

Available since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX6P-2-human RNF146 full length

Plasmid

#132610

Purpose

expresses human RNF146 full length in E. coli

Depositor

Wenqing Xu

Article

DaRosa et al Nature. 2015 Jan 8;517(7533):223-6. doi:

Insert

RNF146 (RNF146 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 17, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pBacMam2-DiEx-LIC-N-flag_huntingtin_full-length_Q139

Plasmid

#111738

Purpose

Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells

Depositor

Cheryl Arrowsmith

Article

Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70

Insert

HTT (HTT Human)

Use

Baculovirus expression

Tags

FLAG

Expression

Mammalian

Mutation

Q139 (polyQ repeat)

Promoter

CMV and P10

Available since

June 25, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pOPINB-OTULIN (full-length, aa 1-352)

Plasmid

#61464

Purpose

Expresses human OTULIN (full-length) in E. coli.

Depositor

David Komander

Article

Keusekotten et al Cell. 2013 Jun 6;153(6):1312-26. doi: 10

Insert

OTULIN (OTULIN Human)

Use

Tags

His6-3C

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pBacMam2-DiEx-LIC-N-flag_huntingtin_full-length_Q19

Plasmid

#111733

Purpose

Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells

Depositor

Cheryl Arrowsmith

Article

Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70

Insert

HTT (HTT Human)

Use

Baculovirus expression

Tags

FLAG

Expression

Mammalian

Mutation

Q19 (polyQ repeat)

Promoter

CMV and P10

Available since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pBacMam2-DiEx-LIC-N-flag_huntingtin_full-length_Q51

Plasmid

#111734

Purpose

Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells

Depositor

Cheryl Arrowsmith

Article

Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70

Insert

HTT (HTT Human)

Use

Baculovirus expression

Tags

FLAG

Expression

Mammalian

Mutation

Q51 (polyQ repeat)

Promoter

CMV and P10

Available since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pOPINK-OTUD1 (full-length, aa 1-481)

Plasmid

#61405

Purpose

Expresses human OTUD1 (full-length) in E. coli.

Depositor

David Komander

Article

Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

Insert

OTUD1 (OTUD1 Human)

Use

Tags

His6-GST-3C

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 18, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pOPINK-OTUD2 (full-length, aa 1-348)

Plasmid

#61409

Purpose

Expresses human OTUD2 (full-length) in E. coli.

Depositor

David Komander

Article

Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

Insert

OTUD2 (YOD1 Human)

Use

Tags

His6-GST-3C

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 18, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q79

Plasmid

#111729

Purpose

Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells

Depositor

Cheryl Arrowsmith

Article

Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70

Insert

HTT (HTT Human)

Use

Baculovirus expression

Tags

FLAG

Expression

Mammalian

Mutation

Q79 (polyQ repeat)

Promoter

CMV and P10

Available since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q54

Plasmid

#111727

Purpose

Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells

Depositor

Cheryl Arrowsmith

Article

Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70

Insert

HTT (HTT Human)

Use

Baculovirus expression

Tags

FLAG

Expression

Mammalian

Mutation

Q54 (polyQ repeat)

Promoter

CMV and P10

Available since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q19

Plasmid

#111741

Purpose

Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells

Depositor

Cheryl Arrowsmith

Article

Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70

Insert

HTT (HTT Human)

Use

Baculovirus expression

Tags

FLAG

Expression

Mammalian

Mutation

Q19 (polyQ repeat)

Promoter

CMV and P10

Available since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pOPINK-OTUD6B (full-length, aa 1-293)

Plasmid

#61418

Purpose

Expresses human OTUD6B (full-length) in E. coli.

Depositor

David Komander

Article

Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

Insert

OTUD6B (OTUD6B Human)

Use

Tags

His6-GST-3C

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pOPINK-OTUD6A (full-length, aa 1-288)

Plasmid

#61416

Purpose

Expresses human OTUD6A (full-length) in E. coli.

Depositor

David Komander

Article

Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

Insert

OTUD6A (OTUD6A Human)

Use

Tags

His6-GST-3C

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
HeLa full-length MLCK-GFP

Plasmid

#46316

Depositor

Anne Bresnick

Article

Dulyaninova et al J Cell Sci. 2004 Mar 15;117(Pt 8):1481-9

Insert

HeLa long myosin light chain kinase (MYLK Human)

Use

Tags

GFP

Expression

Bacterial and Mammalian

Mutation

Promoter

CMV

Available since

July 8, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pBacMam2-DiEx-LIC-N-flag_huntingtin_full-length_Q73

Plasmid

#111724

Purpose

Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells

Depositor

Cheryl Arrowsmith

Article

Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70

Insert

HTT (HTT Human)

Use

Baculovirus expression

Tags

FLAG

Expression

Mammalian

Mutation

Q73 (polyQ repeat)

Promoter

CMV and P10

Available since

Nov. 1, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q109

Plasmid

#111730

Purpose

Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells

Depositor

Cheryl Arrowsmith

Article

Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70

Insert

HTT (HTT Human)

Use

Baculovirus expression

Tags

FLAG

Expression

Mammalian

Mutation

Q109 (polyQ repeat)

Promoter

CMV and P10

Available since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge