We narrowed to 12,023 results for: addgene/1000

1. pcDNA3-teLuc c-myc

   Plasmid

   #100026

   Purpose

   Enhanced red-shifted bioluminescence when paired with diphenylterazine (DTZ)

   Depositor

   Huiwang Ai

   Article

   Yeh et al Nat Methods. 2017 Sep 4. doi: 10.1038/nm

   Insert

   teLuc, a NanoLuc mutant
   

   Use

   Tags

   c-myc

   Expression

   Mammalian

   Mutation

   D19S, D85N, C164H

   Promoter

   CMV

   Available since

   Oct. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. Frb-N-TEV

   Plasmid

   #100095

   Purpose

   SNIPer plasmid, N terminal TEV fused to Frb

   Depositor

   Jim Wells

   Article

   Morgan et al Proc Natl Acad Sci U S A. 2015 Jul 7;112

   Insert

   N-TEV
   

   Use

   Retroviral

   Tags

   Frb and Myc

   Expression

   Mammalian

   Mutation

   N terminal domain

   Promoter

   CMV

   Available since

   Nov. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. FKBP-C-TEV 219

   Plasmid

   #100096

   Purpose

   SNIPer plasmid, C terminal TEV fused to FKBP

   Depositor

   Jim Wells

   Article

   Morgan et al Proc Natl Acad Sci U S A. 2015 Jul 7;112

   Insert

   C-TEV
   

   Use

   Retroviral

   Tags

   FKBP and Myc

   Expression

   Mammalian

   Mutation

   C terminal domain

   Promoter

   CMV

   Available since

   May 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. ECFP-TevS-YPET

   Plasmid

   #100097

   Purpose

   FRET-based ECFP-YPET reporter that is cleaved by TEV

   Depositor

   Jim Wells

   Article

   Gray et al Cell. 2010 Aug 20;142(4):637-46. doi: 10

   Insert

   ECFP-TevS-YPET
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV.CAG.hChR2(H134R)-mCherry.WPRE.SV40

   Plasmid

   #100054

   Purpose

   AAV expression of humanized ChR2 with H134R mutation fused to mCherry driven by CAG promoter for optogenetic activation

   Depositor

   Karl Deisseroth

   Has Service

   AAV1, AAV2, AAV5, and AAV9

   Insert

   channelrhodopsin-2
   

   Use

   AAV

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   H134R mutation in ChR2

   Promoter

   CAG

   Available since

   Jan. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV.CBA.loxP.ArcLightD.2A.nlsmCherry.loxP.WPRE.SV40

   Plasmid

   #100030

   Purpose

   AAV expression of ArcLightD

   Depositor

   Lawrence Cohen

   Article

   Storace et al Sci Rep. 2015 May 13;5:10212. doi: 10.10

   Insert

   ArcLightD
   

   Use

   AAV

   Tags

   2A-mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CBA

   Available since

   Jan. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV.synP.DIO.EGFP.WPRE.hGH

   Plasmid

   #100043

   Purpose

   An AAV vector that expresses EGFP in a Cre-dependent manner (DIO, double floxed inverted open reading frame).

   Depositor

   Ian Wickersham

   Has Service

   AAV9

   Insert

   EGFP
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   synapsin-1

   Available since

   Jan. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. NavBp

   Plasmid

   #100014

   Purpose

   Electrophysiology in mammalian cells: Mammalian expression of a voltage gated ion channel and GFP

   Depositor

   Paul DeCaen, David Clapham

   Article

   DeCaen et al Elife. 2014 Nov 11;3. doi: 10.7554/eLife

   Insert

   NavBp
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. NsvBa

   Plasmid

   #100015

   Purpose

   Electrophysiology in mammalian cells: Mammalian expression of a non-selective voltage gated ion channel and GFP

   Depositor

   Paul DeCaen, David Clapham

   Article

   DeCaen et al Elife. 2014 Nov 11;3. doi: 10.7554/eLife

   Insert

   NsvBa
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAAV.hSynap.ArcLightD.WPRE.SV40

    Plasmid

    #100037

    Purpose

    AAV expression of ArcLightD driven by hSynap promoter

    Depositor

    Vincent Pieribone

    Insert

    ArcLightD
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSynap

    Available since

    Jan. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pENN.AAV.hSynap.Flex.ArcLightDco.WPRE.SV40

    Plasmid

    #100038

    Purpose

    AAV expression of FLEXed ArcLightD driven by hSynap promoter

    Depositor

    Vincent Pieribone

    Insert

    ArcLightDco
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSynap

    Available since

    Jan. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV.CaMKIIa.ChETA(E123T/H134R)-eYFP.WPRE.hGH

    Plasmid

    #100050

    Purpose

    AAV expression of humanized ChR2 with E123T/H134R mutations fused to EYFP driven by CaMKIIa promoter for optogenetic activation

    Depositor

    Karl Deisseroth

    Has Service

    AAV9

    Insert

    hChR2(E123T/H134R)
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    humanized, E123T/H134R

    Promoter

    CaMKIIa

    Available since

    Jan. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-Ef1a-DIO C1V1 (t/t)-TS-mCherry (PV2670)

    Plasmid

    #100061

    Purpose

    AAV expression of ChR1-VChR1 Chimera fused to mCherry driven by Ef1a promoter

    Depositor

    Karl Deisseroth

    Has Service

    AAV9

    Insert

    ChR1-VChR1 Chimera
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    E122T and E162T

    Promoter

    EF1a

    Available since

    Jan. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV.hSynap.ChETA(E123T/H134R)-eYFP.WPRE.hGH

    Plasmid

    #100049

    Purpose

    AAV expression of humanized ChR2 with E123T/H134R mutations fused to EYFP driven by hSynap promoter for optogenetic activation

    Depositor

    Karl Deisseroth

    Has Service

    AAV9

    Insert

    hChR2(E123T/H134R)
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    humanized, E123T/H134R

    Promoter

    hSynap

    Available since

    Jan. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pACT2-Dhr1

    Plasmid

    #100003

    Purpose

    Express yeast Dhr1 with Y2H activation domain fusion

    Depositor

    Susan Baserga

    Article

    Vincent et al RNA. 2017 Oct 20. pii: rna.062927.117. d

    Insert

    Dhr1 (ECM16 Budding Yeast)
    

    Use

    Tags

    Y2H activation domain

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAS2-1-Dhr1

    Plasmid

    #100002

    Purpose

    Express yeast Dhr1 with Y2H binding domain fusion

    Depositor

    Susan Baserga

    Article

    Vincent et al RNA. 2017 Oct 20. pii: rna.062927.117. d

    Insert

    Dhr1 (ECM16 Budding Yeast)
    

    Use

    Tags

    Y2H binding domain

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

17. pDONR221-Sgd1

    Plasmid

    #100000

    Purpose

    Shuttle Sgd1 into Gateway destination vector

    Depositor

    Susan Baserga

    Article

    Vincent et al RNA. 2017 Oct 20. pii: rna.062927.117. d

    Insert

    Sgd1 (SGD1 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDONR221-Bms1

    Plasmid

    #100001

    Purpose

    Shuttle Bms1 into Gateway destination vector

    Depositor

    Susan Baserga

    Article

    Vincent et al RNA. 2017 Oct 20. pii: rna.062927.117. d

    Insert

    Bms1 (BMS1 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET28-his-3C_Tau352

    Plasmid

    #100092

    Purpose

    Expresses human full-length fetal tau protein with the N-terminal 3C-cleavable His6 tag

    Depositor

    Nikolai Sluchanko

    Article

    Tugaeva et al PLoS One. 2017 Jun 2;12(6):e0178933. doi

    Insert

    Fetal microtubule-associated protein tau (MAPT Human)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    wild type sequence

    Promoter

    T7

    Available since

    Sept. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. DNMT3A-dCas9

    Plasmid

    #100090

    Purpose

    DNMT3A fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression

    Depositor

    David Segal

    Article

    O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99

    Insert

    DNMT3A (DNMT3A Human)
    

    Use

    CRISPR

    Tags

    3XFLag-NLS-DNMT3A-dCas9-NLS

    Expression

    Mammalian

    Mutation

    includes aa 602-912

    Promoter

    CMV

    Available since

    Oct. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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